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. 1996 Dec;71(6):3207–3214. doi: 10.1016/S0006-3495(96)79514-0

Thermal history alters cholesterol effect on transition of 1-palmitoyl-2-linoleoyl phosphatidylcholine.

M R Morrow 1, P J Davis 1, C S Jackman 1, K M Keough 1
PMCID: PMC1233809  PMID: 8968591

Abstract

The effect of cholesterol on the bilayer phase behavior of heteroacid phosphatidylcholines with one unsaturated fatty acid depends on the nature of the unsaturated chain. Previous differential scanning calorimetry (DSC) studies showed that 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (16:0-18:2 PC) had a broad, weak transition at about -18 degrees C, which was effectively eliminated by less than 15 mol% cholesterol. Phospholipids with greater and lesser degrees of unsaturation displayed stronger phase transitions and less sensitivity to cholesterol. In this work, deuterium nuclear magnetic resonance has been used to examine the phase behavior of 1-perdeuteriopalmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (16:0-18:2 PC-d31) alone, and with 15 mol % cholesterol. The behavior is found to be sensitive to sample thermal history. Moderately fast cooling (1 degree/h) results in a continuous phase change from a fluid to an ordered phase in the pure lipid. Under similar cooling conditions, the sample containing cholesterol displays increased chain order and a continuous phase change with no apparent isothermal transition. However, when these systems are cooled at a reduced rate (0.3 degree/h), the continuous phase change is pre-empted by a sharp transition into a more ordered phase that gives a deuterium spectrum having intensity at a value of the quadrupole-splitting characteristic of a rigid lattice system. In the pure lipid, this transition effectively coincides with the center of the continuous phase change. Addition of 15 mol % cholesterol lowers the temperature of this sharp transition by about 3 degrees C. These observations provide some insights into the behavior of this system seen using differential scanning calorimetry. Results of deuteron transverse relaxation measurements under these conditions are also reported.

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Selected References

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  1. Davis J. H. Deuterium magnetic resonance study of the gel and liquid crystalline phases of dipalmitoyl phosphatidylcholine. Biophys J. 1979 Sep;27(3):339–358. doi: 10.1016/S0006-3495(79)85222-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis J. H. The description of membrane lipid conformation, order and dynamics by 2H-NMR. Biochim Biophys Acta. 1983 Mar 21;737(1):117–171. doi: 10.1016/0304-4157(83)90015-1. [DOI] [PubMed] [Google Scholar]
  3. Finegold L., Singer M. A. Phosphatidylcholine bilayers: subtransitions in pure and in mixed lipids. Chem Phys Lipids. 1984 Aug;35(3):291–297. doi: 10.1016/0009-3084(84)90053-7. [DOI] [PubMed] [Google Scholar]
  4. Finegold L., Singer M. A. The metastability of saturated phosphatidylcholines depends on the acyl chain length. Biochim Biophys Acta. 1986 Mar 13;855(3):417–420. doi: 10.1016/0005-2736(86)90086-6. [DOI] [PubMed] [Google Scholar]
  5. Hernandez-Borrell J., Keough K. M. Heteroacid phosphatidylcholines with different amounts of unsaturation respond differently to cholesterol. Biochim Biophys Acta. 1993 Dec 12;1153(2):277–282. doi: 10.1016/0005-2736(93)90416-w. [DOI] [PubMed] [Google Scholar]
  6. Keough K. M., Davis P. J. Gel to liquid-crystalline phase transitions in water dispersions of saturated mixed-acid phosphatidylcholines. Biochemistry. 1979 Apr 17;18(8):1453–1459. doi: 10.1021/bi00575a011. [DOI] [PubMed] [Google Scholar]
  7. Keough K. M., Giffin B., Matthews P. L. Phosphatidylcholine-cholesterol interactions: bilayers of heteroacid lipids containing linoleate lose calorimetric transitions at low cholesterol concentration. Biochim Biophys Acta. 1989 Jul 24;983(1):51–55. doi: 10.1016/0005-2736(89)90379-9. [DOI] [PubMed] [Google Scholar]
  8. Lewis R. N., McElhaney R. N. Subgel phases of n-saturated diacylphosphatidylcholines: a Fourier-transform infrared spectroscopic study. Biochemistry. 1990 Aug 28;29(34):7946–7953. doi: 10.1021/bi00486a024. [DOI] [PubMed] [Google Scholar]
  9. Morrow M. R. Transverse nuclear spin relaxation in phosphatidylcholine bilayers containing gramicidin. Biochim Biophys Acta. 1990 Apr 13;1023(2):197–205. doi: 10.1016/0005-2736(90)90414-j. [DOI] [PubMed] [Google Scholar]
  10. Morrow M. R., Whitehead J. P., Lu D. Chain-length dependence of lipid bilayer properties near the liquid crystal to gel phase transition. Biophys J. 1992 Jul;63(1):18–27. doi: 10.1016/S0006-3495(92)81579-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pauls K. P., MacKay A. L., Söderman O., Bloom M., Tanjea A. K., Hodges R. S. Dynamic properties of the backbone of an integral membrane polypeptide measured by 2H-NMR. Eur Biophys J. 1985;12(1):1–11. doi: 10.1007/BF00254089. [DOI] [PubMed] [Google Scholar]
  12. Prosser R. S., Davis J. H., Dahlquist F. W., Lindorfer M. A. 2H nuclear magnetic resonance of the gramicidin A backbone in a phospholipid bilayer. Biochemistry. 1991 May 14;30(19):4687–4696. doi: 10.1021/bi00233a008. [DOI] [PubMed] [Google Scholar]
  13. Tristram-Nagle S., Suter R. M., Sun W. J., Nagle J. F. Kinetics of subgel formation in DPPC: X-ray diffraction proves nucleation-growth hypothesis. Biochim Biophys Acta. 1994 Apr 20;1191(1):14–20. doi: 10.1016/0005-2736(94)90227-5. [DOI] [PubMed] [Google Scholar]
  14. Yeagle P. L. Cholesterol and the cell membrane. Biochim Biophys Acta. 1985 Dec 9;822(3-4):267–287. doi: 10.1016/0304-4157(85)90011-5. [DOI] [PubMed] [Google Scholar]
  15. de Kruyff B., Demel R. A., Slotboom A. J., van Deenen L. L., Rosenthal A. F. The effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study. Biochim Biophys Acta. 1973 Apr 25;307(1):1–19. doi: 10.1016/0005-2736(73)90020-5. [DOI] [PubMed] [Google Scholar]

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