Skip to main content
PLOS One logoLink to PLOS One
. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912

Can working in agriculture have a favorable effect on depressive symptoms? Life satisfaction as a mediator

Feiying He 1,, Xiaoying Li 2,, Xiangchun Xu 3,, Shulin Bao 2, Yanwu Chen 4,5, Hualin Liu 4,5,*, Yuan Yao Chen 4,5,*
Editor: Zahra Lorigooini6
PMCID: PMC12349095  PMID: 40802612

Abstract

Purpose

Several studies have explored the relationship between various aspects of work and the onset of depressive symptoms. However, there is a lack of research focusing on the association between job types and depressive symptoms. This study aims to investigate the impact of agricultural work on depressive symptoms and whether life satisfaction mediates this relationship.

Methods

Data were obtained from the 2015 China Health and Retirement Longitudinal Study (CHARLS) (n = 6856). Participants were categorized based on whether they were engaged in agricultural or non-agricultural work and further classified as self-employed or employed. Depressive symptoms and life satisfaction were assessed using the CES-D and SWLS scales. Logistic regression analysis was used to examine associations, and Baron and Kenny’s mediation test and the Sobel test were used to assess the mediating effect of life satisfaction.

Results

Engaging in agricultural work was positively associated with increased depressive symptoms scores (B = 3.437, p < 0.001), indicating that agricultural work exacerbates depressive symptoms. This effect was partially mediated by life satisfaction.

Conclusions

Self-employed agricultural workers are a high-risk group for depressive symptoms. Additionally, life satisfaction plays a mediating role between type of job and depressive symptoms. Public health recommendations aimed at improving or mitigating depressive symptoms among agricultural workers could focus on enhancing life satisfaction to promote healthier psychological status.

Introduction

Depression is a common mental illness and has now become the biggest health burden worldwide [1,2]. According to the World Health Organization, about 5% of adults suffer from depression worldwide, affecting 280 million people, of whom more than 54 million people in China suffer from depression [3]. The number of people suffering from depression is still on the rise with each passing year. Given the impact of depression on individuals and society, there is a great need for in-depth research on depression.

Research has shown that work is closely related to the development of depression [4], which can be subdivided into work pressure [5], work hours [6,7], work environment [8,9],work support [10],subjective social status [11], and so on, but a lack of research focusing on the association between job type of agricultural and non-agricultural and depression. Among the mainstream treatments for depression, there is a therapy called Horticultural Therapy [12,13], which suggests that gardening and being close to nature can help people with depression to recover. While empirical research has demonstrated a significantly elevated prevalence of depressive symptoms among middle-aged and elderly populations residing in rural China compared to their urban counterparts [14]. This epidemiological phenomenon stands in stark contrast to the theoretical postulates of Horticultural Therapy, which advocate the mental health benefits derived from nature immersion. The findings reveal a complex mechanistic relationship between agrarian occupational environments and psychological well-being, indicating that mere proximity to natural elements fails to comprehensively elucidate the etiological pathways through which agricultural labor influences depressive states. Life satisfaction is an important indicator of subjective well-being, which can reflect an individual’s cognitive evaluation of quality of life. It has been proved that there is an association between life satisfaction and depression in middle-aged and elderly people [15,16], which can be used as an important indicator of mental health and quality of life of middle-aged and elderly people. And some other factors of work have been shown to be associated with life satisfaction [1719]. Therefore, in this study, it is necessary to investigate whether the type of jobs has a correlation with life satisfaction

This study will first examine the association between different types of work and depressive symptoms. Further, it will analyze population subgroups based on work state—self-employed versus employed—in order to provide more targeted public health recommendations. Additionally, life satisfaction will be incorporated into the model as a mediating variable to explore whether job types influence depressive symptoms through the pathway of life satisfaction. It was hypothesized that agricultural work could reduce depressive symptoms by increasing subjects’ life satisfaction. The relationship between farming, life satisfaction, and depressive symptoms was empirically investigated as a way to provide a theoretical basis for national mental health promotion.

Method

Research design

The data comes from the 2015 China Health and Aging Tracking Survey (CHARLS), a large-scale social survey project jointly initiated and undertaken by the National Development Research Institute and the School of Management at Peking University. The database aims to collect tracking survey data on the health, economic and social conditions of China’s middle-aged and elderly population aged 45 and above, providing important data support for the study of China’s aging problem.

The survey in the CHARLS database covers a wide range of aspects such as the health states, lifestyles, economic states, social security, family structures and mental health of middle-aged and elderly people. The survey adopts a multi-stage random sampling method to ensure the representativeness of the sample. The questionnaire was rigorously designed, and internationally recognized indicators and measurement methods were used to ensure the comparability of the data. All subjects signed an informed consent form and were approved by the Ethics Committee of Peking University (IRB00001052–11015).

Ethics approval

All respondents gave informed written consent prior to the interview, and ethical approval for the collection of human subject data was obtained, which was updated annually at the Peking University Institutional Review Board (IRB00001052–11015). No additional ethical approval is required for approved data users. All methods of this study were performed in accordance with the guidelines of the Declaration of Helsinki.

Study population

In the present study, participants were from CHARLS (2015) and the criteria for inclusion of subjects at baseline were subjects who contained a record of type of jobs, assessment of depressive states, assessment of life satisfaction, age, gender, type of residence, work states, marital states, socially engaged activity score, smoking states, drinking states, sleep duration, and prior work states in CHARLS 2015. Of these, missing values for job type records (n = 23), missing values for depressive symptoms (n = 1297) missing values for life satisfaction (n = 1436), and missing values for other covariates (n = 11270) were excluded, and 6856 subjects were ultimately enrolled in the study (Fig 1).

Fig 1. Flowchart ofparticipants selection.

Fig 1

Measurement tools

Job type and work state.

In this study, the population was grouped by type of jobs and work states according to the questionnaire questions of CHARLS 2015 WORK, RETIREMENT AND PENSION. The types of jobs was categorized into those engaged in agricultural work and those engaged in non-agricultural work, and the work states were categorized into self-employed and employed. The exact division steps can be seen in the following figure (Fig 2).

Fig 2. Subgroup division.

Fig 2

Depressive symptoms

Depression state was measured using the 10-item Center for Epidemiological Studies Depression Scale (CES-D), which is now a widely used and proven valid and reliable common indicator of depression [20,21]. Previous studies have shown that this assessment is a sensitive, responsive, valid, and reliable tool for identifying and tracking depressive symptoms in Chinese adults. The CES-D assesses depression through 10 dimensions, including 8 negative emotions (e.g., I feel depressed) and 2 positive emotions (e.g., I am hopeful about the future). Participants were asked to assess how often they felt each of the ten emotions in the past week. Responses to each dimension were scored and coded on a 4-point scale ranging from 0 “little or no time (< 1 day)” to 3 “most or all of the time (5 - 7 days)”. The final CES – D score was calculated by combining the ten dimensions, ranging from 0–30, and was corrected for two positive emotions. The higher the CES – D score, the more severe the depression.

Life satisfaction

Life satisfaction was assessed at baseline by asking participants, “How satisfied are you with your life in general?”. “Life satisfaction was assessed at baseline. This question correlates strongly with the validated 5-item Satisfaction with Life Scale (SWLS) [22] and has been used extensively in previous studies. The five response options are: not at all satisfied, not too satisfied, relatively satisfied, very satisfied, and extremely satisfied. The scores range from 0 ~ 4, with higher scores indicating higher life satisfaction.

Selection of covariates

Based on previous studies, we selected a series of covariates, including demographic characteristics such as age [2325], gender [26,27], type of residential address [28], and marital states [29,30], as well as factors that have been validated by research to be highly correlated with depressive states, such as social activity participation [31], smoking states [3234], drinking states [35]. These covariates were obtained from CHARLS: gender was categorized as 0 (female) and 1 (male), type of residential address was categorized as 0 (urban) and 1 (rural), and marital states were categorized as 0 (unmarried) and 1 (married), and social activity participation was assessed by using the question DA056 in CHARLS 2015, which asked subjects “In the past month, did you engage in the following Social activities” was asked in question DA056 of CHARLS 2015, and scores were assigned according to the social activities selected, with each item accumulating one point, ranging from 0 to 11 points, with higher scores indicating higher participation in social activities. Smoking states were categorized as 0 (non-smokers), 1 (smokers); drinking states were rated using a continuous value score, based on question DA067 in the self-answered CHARLS 2015, with scores ranging from 0 ~ 2, with higher scores indicating more frequent drinking. Prior work states data were derived from the ZF1 loading variable of CHARLS 2015, categorized as 0 (not working at the time of the last round of the survey), 1 (working in non-farming at the time of the last round of the survey), 2 (working in agriculture at the time of the last round of the survey), and 3 (working in both agriculture and non-farming at the time of the last round of the survey).

Methods of statistical analysis

As a preliminary analysis, a presentation of baseline data was conducted. Correlation analyses were then conducted to reveal associations between job type, life satisfaction, and depressive states, as well as with covariates. After clarifying the correlations between variables, we dichotomized depression states for logistic regression and divided the population by job type and job states for subgroup regression analysis. We then conducted mediation effects analyses and performed sensitivity tests. We used Baron and Kenny’s mediation test [36,37] to access the mediating role of life satisfaction. Sobel’s test [37,38] was also used to verify the sensitivity of the mediating effect. Finally, we propose a mediated effects model. The above statistical analysis is based on R-4.3.1.

Result

Sample characteristics before matching

The socio-demographic characteristics of the study subjects are shown in Table 1. A total of 6856 subjects were included in this study, of whom 1663 were engaged in non-agricultural work and 5193 in agricultural work. A significantly higher proportion of subjects were females (48.8%) working in agriculture than those working in non-agriculture (38.7%), and the mean age of agricultural workers (68.2 years) was higher than that of non-agricultural workers (64.3 years). Most of the subjects working in agriculture had rural residential addresses (89.9%) and self-employed working states (90.5%), while most of the subjects working in non-agriculture had urban residential addresses (57.3%) and employed working states (68.9%). There was no significant difference in the marital states of the subjects.

Table 1. Baseline before matching.

Non-agriculture
(N = 1663)
Agriculture
(N = 5193)
p-value
Gender
 Female 643 (38.7%) 2532 (48.8%) <0.001
 Male 1020 (61.3%) 2661 (51.2%)
Age
 Mean (SD) 64.3 (6.85) 68.2 (8.30) <0.001
 Median [Min, Max] 63.0 [28.0, 91.0] 68.0 [28.0, 101]
Address_type
 City 953 (57.3%) 526 (10.1%) <0.001
 Village 710 (42.7%) 4667 (89.9%)
Work_state
 Self-employed 518 (31.1%) 4701 (90.5%) <0.001
 Employed 1145 (68.9%) 492 (9.5%)
Martial_states
 Unmarried 119 (7.2%) 448 (8.6%) 0.0651
 Married 1544 (92.8%) 4745 (91.4%)
CES_D_score
 Mean (SD) 6.17 (5.30) 8.37 (6.38) <0.001
 Median [Min, Max] 5.00 [0, 29.0] 7.00 [0, 30.0]
Life_satisfaction_score
 Mean (SD) 2.44 (0.735) 2.39 (0.774) 0.0108
 Median [Min, Max] 2.00 [0, 4.00] 2.00 [0, 4.00]
Social_activity_score
 Mean (SD) 1.66 (1.19) 1.31 (0.795) <0.001
 Median [Min, Max] 1.00 [1.00, 9.00] 1.00 [0, 8.00]
Smoke_state
 Nonsmoker 1076 (64.7%) 3557 (68.5%) 0.00442
 Smoker 587 (35.3%) 1636 (31.5%)
Drinking_state
 Mean (SD) 0.823 (0.926) 0.694 (0.907) <0.001
 Median [Min, Max] 0 [0, 2.00] 0 [0, 2.00]
Sleep_time
 Mean (SD) 6.50 (1.56) 6.40 (1.95) 0.0263
 Median [Min, Max] 7.00 [0, 12.0] 6.50 [0, 15.0]
Previous_work_type
 Not Work 219 (13.2%) 438 (8.4%) <0.001
 Non-agricultural Work 1047 (63.0%) 261 (5.0%)
 Agricultural Work 218 (13.1%) 3687 (71.0%)
 Both 179 (10.8%) 807 (15.5%)

The mean CES-D scores of agricultural workers were significantly higher than those of non-agricultural workers (agricultural: 8.37, non-agricultural: 6.17). In addition to this, there were significant differences between the two groups of subjects in life satisfaction score (p = 0.0108), social activity participation score (p < 0.001), smoking states (p = 0.00442), drinking states (p < 0.001), sleep duration (p = 0.0263), and prior work states (p < 0.001).

Sample characteristics after matching

After propensity matching scores, the socio-demographic characteristics of the study subjects after matching those who worked in agriculture and those who did not are shown in Table 2. 1,663 people worked in non-agricultural jobs and 1,663 people worked in agriculture after matching. Agricultural workers had a higher proportion of females (69.3% vs. 38.7%, p < 0.001) and a higher mean age (76.1 years vs. 64.3 years, p < 0.001) compared to non-agricultural workers. All agricultural workers lived in rural areas and were mostly self-employed (93.1%), while most non-agricultural workers lived in urban areas (57.3%) and were employed (68.9%). There was no significant difference in the marital states of the subjects.

Table 2. Baseline after matching.

Non-agriculture
(N = 1663)
Agriculture (N = 1663) P-value
Gender
 Female 643 (38.7%) 1152 (69.3%) <0.001
 Male 1020 (61.3%) 511 (30.7%)
Age
 Mean (SD) 64.3 (6.85) 76.1 (5.98) <0.001
 Median [Min, Max] 63.0 [28.0, 91.0] 76.0 [65.0, 101]
Address_type
 City 953 (57.3%) 0 (0%) <0.001
 Village 710 (42.7%) 1663 (100%)
Work_state
 Self-employed 518 (31.1%) 1548 (93.1%) <0.001
 Employed 1145 (68.9%) 115 (6.9%)
Martial_status
 Unmarried 119 (7.2%) 200 (12.0%) <0.001
 Married 1544 (92.8%) 1463 (88.0%)
CES_D_score
 Mean (SD) 6.17 (5.30) 9.79 (6.86) <0.001
 Median [Min, Max] 5.00 [0, 29.0] 8.00 [0, 30.0]
Life_satisfaction_score
 Mean (SD) 2.44 (0.735) 2.37 (0.770) 0.00708
 Median [Min, Max] 2.00 [0, 4.00] 2.00 [0, 4.00]
Social_activity_score
 Mean (SD) 1.66 (1.19) 1.08 (0.458) <0.001
 Median [Min, Max] 1.00 [1.00, 9.00] 1.00 [0, 5.00]
Smoke_state
 Nonsmoker 1076 (64.7%) 1324 (79.6%) <0.001
 Smoker 587 (35.3%) 339 (20.4%)
Drinking_state
 Mean (SD) 0.823 (0.926) 0.515 (0.840) <0.001
 Median [Min, Max] 0 [0, 2.00] 0 [0, 2.00]
Sleep_time
 Mean (SD) 6.50 (1.56) 6.10 (2.20) <0.001
 Median [Min, Max] 7.00 [0, 12.0] 6.00 [0, 15.0]
Previous_work_type
 Not Work 219 (13.2%) 206 (12.4%) <0.001
 Non-agricultural Work 1047 (63.0%) 28 (1.7%)
 Agricultural Work 218 (13.1%) 1343 (80.8%)
 Both 179 (10.8%) 86 (5.2%)

The mean CES-D scores of agricultural workers were significantly higher than those of non-agricultural workers (agricultural: 9.79, non-agricultural: 6.17).

Relevance analysis

In order to understand the relationship between the variables and the mechanism of action, the Spearman’s correlation analysis of the variables is shown in Fig 3. Overall, age was positively correlated with type of job (r = 0.68,p < 0.05), type of residence (r = 0.49, p < 0.05) and social activity participation score (r = 0.25, p < 0.05) and with marital states (r = −0.18, p < 0.05), CES-D score (r = −0.22, p < 0.05) and prior work states (r = −0.49, p < 0.05), while there was no correlation with gender, life satisfaction score, smoking states, drinking states and sleep duration.

Fig 3. Correlation analysis-relationships between variables.

Fig 3

Logistic regression

Next, in order to explain the relationship between the variables and the depressive state and to find the risk factors for the depressive state, a Logistic multi-factorial regression model was developed (Table 3).Logistic regression analysis revealed that being male (OR = 0.52, 95% CI 0.37 to 0.72, p < 0.001), married (OR = 0.47, 95% CI 0.34 to 0.64, p < 0.001), and employed (OR = 0.56, 95% CI 0.40 to 0.79, p < 0.001) were significantly protective against depressive states. Additionally, life satisfaction (OR = 0.41, 95% CI 0.36 to 0.48, p < 0.001), social activity participation (OR = 0.60, 95% CI 0.47 to 0.76, p < 0.001), and sleep duration (OR = 0.77, 95% CI 0.73 to 0.81, p < 0.001) had significant protective effects against depressive states.

Table 3. Logistic regression.

Dependent: CES-D score Low (N = 2858) High (N = 468) OR (multivariable)
Job type Non-agriculture 1554 (54.4%) 109 (23.3%)
Agriculture 1304 (74%) 359 (76.7%) 1.07 (0.68–1.68, p = .774)
Gender Female 1443 (50.5%) 352 (75.2%)
Male 1415 (49.5%) 116 (24.8%) 0.52 (0.37–0.72, p < .001)
Age Mean ± SD 69.7 ± 8.8 73.3 ± 7.5 1.01 (0.99–1.03, p = .422)
Address type City 896 (31.4%) 57 (12.2%)
Village 1962 (68.6%) 411 (87.8%) 1.13 (0.74–1.73, p = .571)
Work state Self-employed 1678 (58.7%) 388 (82.9%)
Employed 1180 (41.3%) 80 (17.1%) 0.56 (0.40–0.79, p < .001)
Martial status Unmarried 226 (7.9%) 93 (19.9%) 0.47 (0.34–0.64, p < .001)
Married 2632 (92.1%) 375 (80.1%)
Life satisfaction score Mean ± SD 2.5 ± 0.7 1.9 ± 0.9 0.41 (0.36–0.48, p < .001)
Social activity score Mean ± SD 1.4 ± 1.0 1.1 ± 0.4 0.60 (0.47–0.76, p < .001)
Smoke state Nonsmoker 2023 (70.8%) 377 (80.6%)
Smoker 835 (29.2%) 91 (19.4%) 1.24 (0.89–1.72, p = .201)
Drinking state Mean ± SD 0.7 ± 0.9 0.4 ± 0.8 0.88 (0.76–1.02, p = .098)
Sleep time Mean ± SD 6.5 ± 1.8 5.2 ± 2.2 0.77 (0.73–0.81, p < .001)
Previous work type Not Work 349 (12.2%) 76 (16.2%)
Non-agricultural Work 1020 (35.7%) 55 (11.8%) 0.50 (0.32–0.79, p = .003)
Agricultural Work 1248 (43.7%) 313 (66.9%) 0.93 (0.67–1.29, p = .673)
Both 241 (8.4%) 24 (5.1%) 0.64 (0.38–1.09, p = .099)

Subgroup regression analysis

In order to explore in more depth the differences in risk factors for depressive states across populations, we divided the population into six subgroups according to two categorization criteria – job type and job states.

Job type subgroups

The subjects were divided into two subgroups, agricultural and non-agricultural, by type of jobs, and the results of the logistic regression are shown in Fig 4. Among smokers, subjects working in non-agricultural jobs compared to those working in agricultural jobs had an approximately 1.80-fold increased risk of developing a depressive state when compared to those working in agricultural jobs (OR = 1.80; 95% CI 1.01 to 3.20; p = 0.047). However, men (agricultural: OR = 0.56; 95% CI 0.37 to 0.85; p < 0.01 Non-agricultural (OR = 0.39; 95% CI 0.22 to 0.69; p < 0.001) and married (agricultural: OR = 0.54; 95% CI 0.37 to 0.69; p < 0.001) non-smokers were at greater risk than non-smokers. 95% CI 0.37 to 0.77; p < 0.001 Non-agricultural: OR = 0.33; 95% CI 0.18 to 0.60; p < 0.001), employed (agricultural: OR = 0.39; 95% CI 0.20 to 0.74; p < 0.01), and married (agricultural: OR = 0.39; 95% CI 0.20 to 0.74; p < 0.01). Non-agricultural: OR = 0.64; 95% CI 0.41 to 0.99; p < 0.05), life satisfaction (agricultural: OR = 0.47; 95% CI 0.39 to 0.56; p < 0.001 Non-agricultural: OR = 0.30; 95% CI 0.22 to 0.56; p < 0.001 95% CI 0.22 to 0.42; p < 0.001), participation in social activities (agricultural: OR = 0.61; 95% CI, 0.42–0.88; p < 0.01 Non-agricultural: OR = 0.52; 95% CI 0.36 to 0.76; p < 0.001), and sleep duration. 0.001) and sleep duration (agricultural: OR = 0.78; 95% CI 0.74 to 0.83; p < 0.001 Non-agricultural: OR = 0.71; 95% CI 0.62 to 0.82; p < 0.001) were significant protective factors for both subgroups.

Fig 4. Subgroup analysis by job type-risk factors for depressive states among agricultural and non-agricultural workers.

Fig 4

Work state subgroups

Subjects were categorized into four subgroups, self-employed non-farmers (A), self-employed farmers (B), employed non-farmers (C), and employed farmers (D), based on the dual criteria of job states and job type, and the results of the logistic regression are shown in Fig 5.

Fig 5. Subgroup analysis by work state-risk factors for depressive states among self-employed and employed workers.

Fig 5

As can be seen from the figure, in the group with non-agricultural jobs, self-employed subjects had only two significant protective factors for life satisfaction (OR = 0.24; 95%CI 0.14 to 0.42; p < 0.001) and sleep duration (OR = 0.75; 95%CI 0.60 to 0.93; p = 0.01) in comparison to employed subjects, while employed workers had only two significant protective factors in addition to life satisfaction (OR = 0.35; 95%CI 0.24 to 0.51; p < 0.001) and sleep duration (OR = 0.69; 95%CI 0.58 to 0.82; p < 0.001), there were two significant protective factors for employed workers in addition to male (OR = 0.28; 95%CI 0.12 to 0.63; p < 0.01), married (OR = 0.26; 95%CI 0.13 to 0.53; p < 0.001) and social activity participation (OR = 0.45; 95%CI 0.27 to 0.76; p < 0.01), which were three specific protective factors.

In contrast, in the agriculture group, there were no significant protective factors for employed subjects, while men (OR = 0.53; 95% CI 0.35 to 0.81; p < 0.01), married people (OR = 0.52; 95% CI 0.36 to 0.76; p < 0.001), life satisfaction (OR = 0.45; 95% CI 0.38 to 0.54; p < 0.001), social activity participation (OR = 0.64; 95% CI 0.43 to 0.95; p < 0.05), and sleep duration (OR = 0.78; 95% CI 0.73 to 0.82; p < 0.001) were significant protective factors for self-employed workers.

Mediation model analysis and test results

Baron and Kenny’s stepwise regression was used to verify the mediating role of life satisfaction between job type (agricultural vs. non-agricultural) and depressive states, as shown in Table 4. The total effect of job type on depressive states (Path c) was significant (B = 3.622, p < 0.001). Life satisfaction scores showed a significant negative correlation with job type (Path a: B = −0.070, p < 0.001) and depressive states (Path b: B = −2.641, p < 0.001). The indirect effect mediated by life satisfaction (Path a*b) was 0.186 (SE = 0.070, 95% CI 0.0492 to 0.320, p < 0.01), indicating a significant indirect effect. After controlling for the mediator variable life satisfaction, the coefficient of job type on depressive states (Path c’: B = 3.437, p < 0.001) remained significant, indicating that the direct effect was also significant and that the effect of job type on depressive states were partially mediated by life satisfaction.The final mediation model is shown in Fig 6.

Table 4. Mediation analysis.

Variable Path c Path a Path c’ and Path b Path a*b
B SE B SE B SE B SE LLCI ULCI
3.622*** 0.213 −0.070*** 0.026 3.437*** 0.201 0.186** 0.070 0.0492 0.320

Note:*p < 0.05;**p < 0.01; ***p < 0.001

Fig 6. Mediation model.

Fig 6

As shown in Table 5, the estimate of ACME (Average Causal Mediation Effects) was 0.1858, indicating that life satisfaction as a mediating variable produced a significant positive indirect effect between job type and depressive state, suggesting that agricultural workers increased their depressive state by 0.1858 standard deviations through life satisfaction. The mediating effect explained 5.13% of the total effect (B = 3.6224, p < 0.001), suggesting that the level of life satisfaction scores played a role in explaining the role of job type on depressive states.

Table 5. Causal mediation analysis.

Estimate 95% CI Lower 95% CI Upper p-value
ACME 0.1858 0.0492 0.32 0.002**
ADE 3.4366 3.0224 3.84 < 0.001***
Total Effect 3.6224 3.2044 4.03 < 0.001***
Prop.Mediated 0.0513 0.0141 0.09 0.002**

Note:*p < 0.05; **p < 0.001

The sensitivity analysis results are shown in Figure Fig 7. The X-axis is the sensitivity parameter Rho and the Y-axis is the value of the mediating effect. When the point estimate of the average causal mediating effect ACME is 0, i.e., the absolute value of rho when the mediating effect disappears is 0.3, which indicates that the confounding effect is stronger, i.e., the results of this mediating effect model are more reliable.

Fig 7. Sensitivity analysis.

Fig 7

Discussion

This cross-sectional study looks at the effect of job type, whether or not one works in agriculture on depressive states among middle-aged and older adults over the age of 45. Based on a series of regression and mediation analyses from the CHARLS national survey, the findings suggest that agricultural workers have significantly higher depression scores than non-agricultural workers, while life satisfaction partially mediates the association between job type and depression.

Our findings indicate that subjects working in agriculture had higher mean CES-D scores than those working in non-agricultural jobs, suggesting that depressive states are more severe in agricultural jobs, which is not consistent with our hypothesis based on horticultural therapy. Through further Logistic multifactorial regression analysis, we could observe that the type of jobs did not play a role in the occurrence of depressive states under the consideration of the inclusion of other covariates, suggesting that the facilitating effect of agricultural work on depressive states was in the presence of other exposure factors. Previous studies have indicated that exposure factors such as pesticide exposure [3941], social activities [42], and social support [43] are associated with depressive states among agricultural workers, whereas in the present study, the researcher’s baseline showed that life satisfaction scores and social activity participation scores of agricultural workers were lower than those of non-agricultural workers, and further subgroup regression analyses of the type of job with or without farming it can be observed that the increase in life satisfaction and social activity participation of agricultural workers reduces the risk of the occurrence of depressive states. Thus, we can propose the idea that lower life satisfaction and social activity participation are risk factors for the development of depression among agricultural workers. The reasons for low life satisfaction among agricultural workers are complex, primarily influenced by the following factors:(1) Economic Pressure [41,44]: Agricultural workers’ incomes are generally less stable compared to those in non-agricultural occupations, as they are highly susceptible to weather changes, market fluctuations, and other external factors. The seasonal and uncertain nature of agricultural income can impose substantial financial stress, leading to lower life satisfaction.(2) Occupational Health Risks [45]: Agricultural workers face high occupational health risks, including long working hours, exposure to adverse weather conditions, and pesticide exposure mentioned earlier. These health risks negatively impact both physical and mental well-being, reducing overall life satisfaction.(3) Social Isolation and Lack of Social Support [44]: Agricultural workers are often geographically isolated due to the nature of their work, resulting in limited social interactions and relationships. This isolation contributes to lower levels of social participation. The lack of social support and limited opportunities for interaction may lead to feelings of loneliness and depressive symptoms, further impacting life satisfaction.(4) Mental Health Issues and Cultural Stigma [45]: In rural areas, mental health issues are still subject to cultural stigma, which makes it difficult for agricultural workers experiencing mental health challenges to seek help. The lack of mental health support resources can exacerbate these issues, leading to further declines in life satisfaction.

The baseline shows that compared to non-agricultural workers, agricultural workers live in rural areas. Urbanization has been a trend in the world, and China has followed the world trend and entered the process of rapid urbanization [4648], which has led to the center of gravity of socio-economic activities being located in the city, resulting in a dramatic change in living environments and living factors. Therefore, the difference between urban and rural life affects mental health to a certain extent, and according to existing studies [49,50] we can point out that the main reason for this effect is that the difference between urban and rural living space and socio-economics makes urban areas have more diversified social activities and more abundant social resources compared to rural areas, which leads to a significant increase in the life satisfaction of urban residents. The social resources available in cities include education [49], health care services [51], pension benefits [49], social infrastructure, and so on. A high level of education can make middle-aged and elderly people have good adaptability in the face of adversity, and has been shown that the education of middle-aged and elderly people will affect the education of their own offspring, which in turn affects the intergenerational support for the elderly, and thus has an impact on the symptoms of depression among the elderly [52];The lack of medical resources in rural areas prevents middle-aged and elderly people from seeking timely medical treatment when they face a series of health problems, which can lead to functional disabilities, and it has been pointed out in an empirical study that functional disabilities of middle-aged and elderly people reduce life satisfaction and aggravate their depressive symptoms [16]; health insurance and pension insurance coverage is higher in urban areas, which can alleviate the economic pressure to a certain extent. This suggests that living in cities helps to increase life satisfaction [53]. There is also evidence from Asia that participation in social activities to increase social engagement has a positive impact on the mental health of middle-aged and older adults [42,51].Current studies indicate a significant negative correlation between digital village construction and depressive symptoms among rural middle-aged and older adults, with digital village construction alleviating depression through income, consumption, and cognitive welfare effects [14]. Based on these findings, the following measures can effectively leverage the three welfare effects of digital village construction to reduce depressive risks among rural agricultural workers and promote healthy aging: 1) enhancing digital infrastructure (e.g., improving rural internet coverage and e-commerce platforms to boost income) [54]; 2) organizing digital literacy training to strengthen cognitive abilities [55,56]; 3) optimizing logistics and online services to facilitate consumption [57].

Our findings suggest that males are a protective factor for the occurrence of depressive states in both the farming and non-farming populations, indicating that there is a gender difference in the occurrence of depressive states and that females are at a higher risk for their occurrence, which is consistent with the findings of previous studies [5860]. There are many factors that contribute to the high prevalence of depressive states in the female population, including a combination of biological, psychological, and sociocultural factors. Biologically, women are more sensitive to hormonal fluctuations associated with the reproductive cycle, such as hormonal fluctuations (menstrual cycle, postpartum, and menopause) that can affect mood thus leading to the occurrence of premenstrual irritability disorder and postpartum depressive states, among others [5961]; psychology points out that women are more prone to recurring thoughts of negativity [59], which may be associated with an increased risk of depressive states; and in the process of growing up, the Gender roles and societal expectations place additional stress on women, with sexual violence [62], sexual abuse [60], interpersonal violence, stress exposure and stress susceptibility [33] at the micro level, and gender inequality [63,64] at the macro level, all of which increase the risk for the development of depressive states in women. Marital states can also have a significant impact on an individual’s emotional health, and there have been precedent results demonstrating that marriage is a protective factor for depressive states [6567], a finding that is consistent with our findings. There is a positive correlation between marriage and mental health, the main reason is that marriage provides emotional fulfillment and social support, the intimate relationship between marriage partners can provide each other with emotional support and encouragement, help each other to cope with setbacks and difficulties in life, reduce loneliness and social isolation, and enhance mental health. In addition to this marriage implies the sharing of economic resources such as housing, income and property, which can reduce financial stress and anxiety. However, it is worth noting that marital quality plays an important role in moderating the effects of marriage on mental health [65].

In order to better provide targeted recommendations for the adjustment of workers’ depressive state, we further conducted subgroup regression analysis based on whether or not they were self-employed, and the results showed that in the agricultural population, self-employed people had more protective factors than employed people, while in the non-agricultural population, employed people had more protective factors than self-employed people. According to the conclusion of the previous study [58], self-employed workers have a greater risk of depression. We analyze that the causes of depression among self-employed workers may lie in the following points: 1) Unstable source of economic income: self-employed workers, whether they work in agriculture or non-agriculture, need to be responsible for their own work profit and loss, and the economic pressure is high, while in comparison with the employed workers’ source of economic income is more stable, with better welfare benefits such as health insurance and health care, and the benefits are better. better welfare benefits, such as higher coverage of health insurance and pension insurance [68]; 2) Blurred work-life boundaries: the uncertainty of working hours often takes up time for private life, resulting in insufficient time for rest and relaxation; 3) High work intensity: in order to maintain continuity and development of their work business, self-employed workers need to spend more time and energy than employed workers; 4) Self-employment is mostly in agriculture, construction, mining, etc., and poorer workplace exposure exposes workers to higher physical and mental health risks [58,69]; 5) And self-employed workers usually lack social support from organizations, which also increases their risk of depression [58]. For the agricultural self-employed and non-agricultural employed people, there are several suggestions we proposed: 1) Improve life satisfaction by improving the quality of life [70,71]; 2) Participate in more social activities to enhance the interaction with friends and social support [42,43,51]; 3) Extend the sleep time and develop good sleep habits to get sufficient physical and mental rest and relaxation [7274]. Moreover, for the government to complement public health policies for agricultural workers, we proposed the following suggestions: 1) Construct more rural fitness and recreational facilities; 2) Conduct more mental health screening activities in rural areas; 3) Implement policies to improve the incomes of agricultural workers in rural areas.

According to the results of BK stepwise regression, it can be observed that there is a partial mediating effect of life satisfaction between the level of depressive state and the type of jobs, i.e., the negative correlation between life satisfaction and the type of jobs indicates that the life satisfaction of agricultural workers is lower than that of non-agricultural workers, and the negative correlation between life satisfaction and depressive state indicates that the risk of depressive state can be reduced by improving life satisfaction, which indicates that agricultural workers have low life satisfaction due to low depressive state. The negative correlation between life satisfaction and depression indicates that the risk of depression can be reduced by increasing life satisfaction, suggesting that agricultural workers are depressed due to low life satisfaction. In terms of the mechanism of depression, studies have shown that there is a close association between depression and life satisfaction, and that the occurrence of depressive symptoms is often accompanied by a decrease in life satisfaction [75], and people with low life satisfaction are more likely to experience depression [76]; the results of a priori RCT have shown that depression symptoms can be significantly reduced by increasing life satisfaction [50], and the results of another RCT found that an active psychological intervention, if conducted for a year, was effective in reducing depressive symptoms and increasing life satisfaction [75]. All of these findings further support the results of our mediation analysis and are consistent with the findings of the regression analysis.

This study has several limitations. First, it is based on cross-sectional data from the 2015 China Health and Retirement Longitudinal Study (CHARLS), which limits the ability to establish a clear temporal sequence between agricultural employment and depressive symptoms. Consequently, reverse causality cannot be ruled out; for instance, individuals with depression may be more inclined to engage in agricultural work. Longitudinal designs are essential for understanding the temporal relationship between job type and mental health outcomes. Existing longitudinal investigations have illuminated that depressive symptoms are not static; rather, they can fluctuate over time, which cross-sectional studies may not adequately represent. Longitudinal research highlights the dynamic relationship between job type and mental health, with studies showing that unstable employment can lead to increased depressive symptoms over time [77] and work-related stressors can exacerbate mental health issues [78]. Future research should employ longitudinal data to more accurately elucidate causal relationships between variables. Second, a substantial number of samples (n = 11,270) were excluded due to missing covariate data, which may introduce selection bias. Future studies should consider employing multiple imputation techniques to handle missing data and conduct sensitivity analyses to assess the robustness of the findings. Additionally, the classification of occupational types was simplistic, dividing them merely into “agricultural” and “non-agricultural,” failing to capture the heterogeneity within agricultural work, such as subsistence versus commercial farming and land ownership differences. Future research should refine occupational classifications to more comprehensively understand the impact of occupational types on depressive symptoms. The study also employed logistic regression to analyze depressive symptoms, a common outcome variable, which may overestimate odds ratios. Future studies might consider alternative regression models, such as Poisson regression, to obtain more accurate estimates. Although this study found that life satisfaction had a mediating effect between agricultural work and depressive symptoms, the effect was weak, accounting for only 5.13% of the total effect. This may be due to the omission of other potential mediating variables, such as economic stability and pesticide exposure. Future research should explore additional mediators to more fully understand the mechanisms by which agricultural work influences depressive symptoms. Finally, the 2015 data used in this study may not capture recent socioeconomic changes in rural China, such as digital development and policy shifts. Additionally, mental health research involves sensitive information, necessitating careful ethical considerations to ensure participant privacy and informed consent during data collection and analysis. Future studies should focus on the temporal relevance of data and enhance ethical review and participant protection measures.

Conclusion

Our study investigated the relationship between the type of jobs, whether farming or not, and depressive states, emphasizing through subgroup analysis that self-employed job type, agricultural job and sex as female are risk factors for developing depressive states and developing depression. In addition, life satisfaction mediated the relationship between the type of job and the level of depressive state, a result that highlights the complex association between the type of job and depressive state.

Therefore, based on the results of our logistic regression and mediation analyses, public health recommendations that can intervene in the occurrence of depressive states or alleviate existing depressive states by improving life satisfaction can be made for agricultural workers in order to help agricultural workers have a healthier psychological state. Future research could focus on the mechanisms by which differences in specific parameters of life satisfaction under different job type classifications affect depressive states and whether other mediating variables are also present.

Data Availability

All relevant data can be found at the following website: http://CHARLS.pku.edu.cn/en. We conducted the study just through downloading the three questionaires called "Demographic_Background," "Health_Status_and_Functioning" and "Work_Retirement_and_Pension" from CHARLS 2015, all of which can be found at: https://charls.charlsdata.com/pages/data/111/en.html.

Funding Statement

This research is supported by Key Laboratory of Philosophy and Social Sciences of Colleges and Universities in Guangdong Province (2023WSYS005, 2023WSYS007), the 13th Five-Year Plan of Guangdong Province for Philosophy and Social Sciences (GD20XGL42). There is no funders participating in the study or in the process of study.

References

  • 1.Doris A, Ebmeier K, Shajahan P. Depressive illness. Lancet. 1999;354(9187):1369–75. doi: 10.1016/S0140-6736(99)03121-9 [DOI] [PubMed] [Google Scholar]
  • 2.Mental health: A world of depression. Nature. Available from: https://www.nature.com/articles/515180a. Accessed 2024 June 25.
  • 3.Wu P, Wang R, Lin H, Zhang F, Tu J, Sun M. Automatic depression recognition by intelligent speech signal processing: a systematic survey. CAAI Trans on Intel Tech. 2022;8(3):701–11. doi: 10.1049/cit2.12113 [DOI] [Google Scholar]
  • 4.Liu Y, Yang X, Wu Y, Xu Y, Zhong Y, Yang S. The relationship between job satisfaction and depressive symptoms among Chinese adults aged 35-60 years: the mediating role of subjective well-being and life satisfaction. Int J Environ Res Public Health. 2023;20(3):2023. doi: 10.3390/ijerph20032023 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.He S-C, Wu S, Wang C, Du X-D, Yin G, Jia Q, et al. Interaction between job stress and the BDNF Val66Met polymorphism affects depressive symptoms in Chinese healthcare workers. J Affect Disord. 2018;236:157–63. doi: 10.1016/j.jad.2018.04.089 [DOI] [PubMed] [Google Scholar]
  • 6.Song X, Fu W, Liu X, Luo Z, Wang R, Zhou N, et al. Mental health status of medical staff in emergency departments during the Coronavirus disease 2019 epidemic in China. Brain Behav Immun. 2020;88:60–5. doi: 10.1016/j.bbi.2020.06.002 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Bryant-Genevier J, Rao CY, Lopes-Cardozo B, Kone A, Rose C, Thomas I, et al. Symptoms of Depression, Anxiety, Post-Traumatic Stress Disorder, and Suicidal Ideation Among State, Tribal, Local, and Territorial Public Health Workers During the COVID-19 Pandemic - United States, March-April 2021. MMWR Morb Mortal Wkly Rep. 2021;70(26):947–52. doi: 10.15585/mmwr.mm7026e1 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Siegrist J, Wege N. Adverse psychosocial work environments and depression-a narrative review of selected theoretical models. Front Psychiatry. 2020;11:66. doi: 10.3389/fpsyt.2020.00066 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Shiga K, Izumi K, Minato K, Yoshimura M, Kitazawa M, Hanashiro S, et al. Association of work environment with stress and depression among Japanese workers. Work. 2022;72(4):1321–35. doi: 10.3233/WOR-210356 [DOI] [PubMed] [Google Scholar]
  • 10.Jia CX, Cheung C-K, Fu C. Work support, role stress, and life satisfaction among chinese social workers: the mediation role of work-family conflict. Int J Environ Res Public Health. 2020;17(23):8881. doi: 10.3390/ijerph17238881 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.The relationship between job satisfaction and depressive symptoms in Chinese men: a moderated multiple mediation model. JOMH. 2024;20(4):80. doi: 10.22514/jomh.2024.056 [DOI] [Google Scholar]
  • 12.Xu M, Lu S, Liu J, Xu F. Effectiveness of horticultural therapy in aged people with depression: a systematic review and meta-analysis. Front Public Health. 2023;11:1142456. doi: 10.3389/fpubh.2023.1142456 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Soga M, Gaston KJ, Yamaura Y. Gardening is beneficial for health: a meta-analysis. Prev Med Rep. 2016;5:92–9. doi: 10.1016/j.pmedr.2016.11.007 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Wan S, Zhang P, Chen S, Yang Y. Digital village construction and depressive symptoms in rural middle-aged and older adults: evidence from China. J Affect Disord. 2025;372:200–9. doi: 10.1016/j.jad.2024.12.026 [DOI] [PubMed] [Google Scholar]
  • 15.Gigantesco A, Fagnani C, Toccaceli V, Stazi MA, Lucidi F, Violani C, et al. The relationship between satisfaction with life and depression symptoms by gender. Front Psychiatry. 2019;10:419. doi: 10.3389/fpsyt.2019.00419 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Li A, Wang D, Lin S, Chu M, Huang S, Lee C-Y, et al. Depression and life satisfaction among middle-aged and older adults: mediation effect of functional disability. Front Psychol. 2021;12:755220. doi: 10.3389/fpsyg.2021.755220 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Ilies R, Schwind KM, Wagner DT, Johnson MD, DeRue DS, Ilgen DR. When can employees have a family life? The effects of daily workload and affect on work-family conflict and social behaviors at home. J Appl Psychol. 2007;92(5):1368–79. doi: 10.1037/0021-9010.92.5.1368 [DOI] [PubMed] [Google Scholar]
  • 18.Yang S, Chen L, Bi X. Overtime work, job autonomy, and employees’ subjective well-being: evidence from China. Front Public Health. 2023;11:1077177. doi: 10.3389/fpubh.2023.1077177 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Parida S, Aamir A, Alom J, Rufai TA, Rufai SR. British doctors’ work-life balance and home-life satisfaction: a cross-sectional study. Postgrad Med J. 2021;:postgradmedj-2021-141338. doi: 10.1136/postmj/postgradmedj-2021-141338 [DOI] [PubMed] [Google Scholar]
  • 20.Boey KW. Cross-validation of a short form of the CES-D in Chinese elderly. Int J Geriat Psychiatry. 1999;14(8):608–17. doi: [DOI] [PubMed] [Google Scholar]
  • 21.Irwin M, Artin KH, Oxman MN. Screening for depression in the older adult: criterion validity of the 10-item Center for Epidemiological Studies Depression Scale (CES-D). Arch Intern Med. 1999;159(15):1701–4. doi: 10.1001/archinte.159.15.1701 [DOI] [PubMed] [Google Scholar]
  • 22.Cheung F, Lucas RE. Assessing the validity of single-item life satisfaction measures: results from three large samples. Qual Life Res. 2014;23(10):2809–18. doi: 10.1007/s11136-014-0726-4 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Coryell W, Solomon D, Leon A, Fiedorowicz JG, Schettler P, Judd L, et al. Does major depressive disorder change with age? Psychol Med. 2009;39(10):1689–95. doi: 10.1017/S0033291709005364 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Hegeman JM, Kok RM, van der Mast RC, Giltay EJ. Phenomenology of depression in older compared with younger adults: meta-analysis. Br J Psychiatry. 2012;200(4):275–81. doi: 10.1192/bjp.bp.111.095950 [DOI] [PubMed] [Google Scholar]
  • 25.Zhu Y, Li C, Xie W, Zhong B, Wu Y, Blumenthal JA. Trajectories of depressive symptoms and subsequent cognitive decline in older adults: a pooled analysis of two longitudinal cohorts. Age Ageing. 2022;51(1):afab191. doi: 10.1093/ageing/afab191 [DOI] [PubMed] [Google Scholar]
  • 26.Piccinelli M, Wilkinson G. Gender differences in depression: critical review. Br J Psychiatry. 2000;177:486–92. doi: 10.1192/bjp.177.6.486 [DOI] [PubMed] [Google Scholar]
  • 27.Parker G, Brotchie H. Gender differences in depression. Int Rev Psychiatry. 2010;22(5):429–36. doi: 10.3109/09540261.2010.492391 [DOI] [PubMed] [Google Scholar]
  • 28.Julien D, Richard L, Gauvin L, Kestens Y. Neighborhood characteristics and depressive mood among older adults: an integrative review. Int Psychogeriatr. 2012;24(8):1207–25. doi: 10.1017/S1041610211002894 [DOI] [PubMed] [Google Scholar]
  • 29.Buckman JEJ, Saunders R, Stott J, Arundell L-L, O’Driscoll C, Davies MR, et al. Role of age, gender and marital status in prognosis for adults with depression: an individual patient data meta-analysis. Epidemiol Psychiatr Sci. 2021;30:e42. doi: 10.1017/S2045796021000342 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Min J, Yorgason JB, Fast J, Chudyk A. The impact of spouse’s illness on depressive symptoms: the roles of spousal caregiving and marital satisfaction. J Gerontol B Psychol Sci Soc Sci. 2020;75(7):1548–57. doi: 10.1093/geronb/gbz017 [DOI] [PubMed] [Google Scholar]
  • 31.Ryu E, Jenkins GD, Wang Y, Olfson M, Talati A, Lepow L, et al. The importance of social activity to risk of major depression in older adults. Psychol Med. 2023;53(6):2634–42. doi: 10.1017/S0033291721004566 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Pasco JA, Williams LJ, Jacka FN, Ng F, Henry MJ, Nicholson GC, et al. Tobacco smoking as a risk factor for major depressive disorder: population-based study. Br J Psychiatry. 2008;193(4):322–6. doi: 10.1192/bjp.bp.107.046706 [DOI] [PubMed] [Google Scholar]
  • 33.Fluharty M, Taylor AE, Grabski M, Munafò MR. The association of cigarette smoking with depression and anxiety: a systematic review. Nicotine Tob Res. 2017;19(1):3–13. doi: 10.1093/ntr/ntw140 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Munafò MR, Araya R. Cigarette smoking and depression: a question of causation. Br J Psychiatry. 2010;196(6):425–6. doi: 10.1192/bjp.bp.109.074880 [DOI] [PubMed] [Google Scholar]
  • 35.Haynes JC, Farrell M, Singleton N, Meltzer H, Araya R, Lewis G, et al. Alcohol consumption as a risk factor for anxiety and depression: results from the longitudinal follow-up of the National Psychiatric Morbidity Survey. Br J Psychiatry. 2005;187:544–51. doi: 10.1192/bjp.187.6.544 [DOI] [PubMed] [Google Scholar]
  • 36.Baron RM, Kenny DA. The moderator-mediator variable distinction in social psychological research: conceptual, strategic, and statistical considerations. J Pers Soc Psychol. 1986;51(6):1173–82. doi: 10.1037//0022-3514.51.6.1173 [DOI] [PubMed] [Google Scholar]
  • 37.Wang J, Zhang J, Lin H, Han Y, Tu J, Nie X. Economic development, weak ties, and depression: evidence from China. J Affect Disord. 2023;334:246–57. doi: 10.1016/j.jad.2023.04.097 [DOI] [PubMed] [Google Scholar]
  • 38.Sobel ME. Asymptotic confidence intervals for indirect effects in structural equation models. Sociological Methodol. 1982;13:290. doi: 10.2307/270723 [DOI] [Google Scholar]
  • 39.Cancino J, Soto K, Tapia J, Muñoz-Quezada MT, Lucero B, Contreras C, et al. Occupational exposure to pesticides and symptoms of depression in agricultural workers. A systematic review. Environ Res. 2023;231(Pt 2):116190. doi: 10.1016/j.envres.2023.116190 [DOI] [PubMed] [Google Scholar]
  • 40.Frengidou E, Galanis P, Malesios C. Pesticide exposure or pesticide poisoning and the risk of depression in agricultural populations: a systematic review and meta-analysis. J Agromedicine. 2024;29(1):91–105. doi: 10.1080/1059924X.2023.2278801 [DOI] [PubMed] [Google Scholar]
  • 41.Zanchi MM, Marins K, Zamoner A. Could pesticide exposure be implicated in the high incidence rates of depression, anxiety and suicide in farmers? A systematic review. Environ Pollut. 2023;331(Pt 2):121888. doi: 10.1016/j.envpol.2023.121888 [DOI] [PubMed] [Google Scholar]
  • 42.Min J, Ailshire J, Crimmins EM. Social engagement and depressive symptoms: do baseline depression status and type of social activities make a difference?. Age Ageing. 2016;45(6):838–43. doi: 10.1093/ageing/afw125 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Wan Mohd Azam WMY, Din NC, Ahmad M, Ghazali SE, Ibrahim N, Said Z, et al. Loneliness and depression among the elderly in an agricultural settlement: mediating effects of social support. Asia Pac Psychiatry. 2013;5 Suppl 1:134–9. doi: 10.1111/appy.12061 [DOI] [PubMed] [Google Scholar]
  • 44.Daghagh Yazd S, Wheeler SA, Zuo A. Key Risk factors affecting farmers’ mental health: a systematic review. Int J Environ Res Public Health. 2019;16(23):4849. doi: 10.3390/ijerph16234849 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Hagen BNM, Albright A, Sargeant J, Winder CB, Harper SL, O’Sullivan TL, et al. Research trends in farmers’ mental health: a scoping review of mental health outcomes and interventions among farming populations worldwide. PLoS One. 2019;14(12):e0225661. doi: 10.1371/journal.pone.0225661 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Bettencourt L, West G. A unified theory of urban living. Nature. 2010;467(7318):912–3. doi: 10.1038/467912a [DOI] [PubMed] [Google Scholar]
  • 47.Bai X, Shi P, Liu Y. Society: Realizing China’s urban dream. Nature. 2014;509(7499):158–60. doi: 10.1038/509158a [DOI] [PubMed] [Google Scholar]
  • 48.Yang XJ. China’s rapid urbanization. Science. 2013;342(6156):310. doi: 10.1126/science.342.6156.310-a [DOI] [PubMed] [Google Scholar]
  • 49.Li LW, Liu J, Xu H, Zhang Z. Understanding rural-urban differences in depressive symptoms among older adults in China. J Aging Health. 2016;28(2):341–62. doi: 10.1177/0898264315591003 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 50.Stier AJ, Schertz KE, Rim NW, Cardenas-Iniguez C, Lahey BB, Bettencourt LMA, et al. Evidence and theory for lower rates of depression in larger US urban areas. Proc Natl Acad Sci U S A. 2021;118(31):e2022472118. doi: 10.1073/pnas.2022472118 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 51.Chang KS. Economic development with limited supplies of family labor: Chinese peasant families in balancing demographic and economic requisites. Korea J Popul Dev. 1991;20(1):47–76. [PubMed] [Google Scholar]
  • 52.Pei Y, Cong Z, Wu B. Education, adult children’s education, and depressive symptoms among older adults in rural China. Soc Sci Med. 2020;253:112966. doi: 10.1016/j.socscimed.2020.112966 [DOI] [PubMed] [Google Scholar]
  • 53.Chen L, Chang L, Lin H, Tu J, Chen X, Han Y. Depressive disorder benefits of cities: evidence from the China. J Affect Disord. 2024;350:420–7. doi: 10.1016/j.jad.2024.01.105 [DOI] [PubMed] [Google Scholar]
  • 54.Mitchell LM, Joshi U, Patel V, Lu C, Naslund JA. Economic evaluations of internet-based psychological interventions for anxiety disorders and depression: a systematic review. J Affect Disord. 2021;284:157–82. doi: 10.1016/j.jad.2021.01.092 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 55.Biagianti B, Foti G, Di Liberto A, Bressi C, Brambilla P. CBT-informed psychological interventions for adult patients with anxiety and depression symptoms: a narrative review of digital treatment options. J Affect Disord. 2023;325:682–94. doi: 10.1016/j.jad.2023.01.057 [DOI] [PubMed] [Google Scholar]
  • 56.Chai Y, Xian G, Wang M, Guo L, Luo S. Aging wisely: the impact of Internet use on older adults’ mental health. J Affect Disord. 2024;364:139–45. doi: 10.1016/j.jad.2024.08.076 [DOI] [PubMed] [Google Scholar]
  • 57.Ji T, Li X, Meng G, Gu Y, Zhang Q, Liu L, et al. The association between banana consumption and the depressive symptoms in Chinese general adult population: a cross-sectional study. J Affect Disord. 2020;264:1–6. doi: 10.1016/j.jad.2019.12.008 [DOI] [PubMed] [Google Scholar]
  • 58.Chen S, Wang Y, She R. Prevalence and gender disparity of those who screen positive for depression in China by the classification of the employer and industry: a cross-sectional, population-based study. BMC Psychiatry. 2023;23(1):62. doi: 10.1186/s12888-023-04557-7 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Kuehner C. Why is depression more common among women than among men?. Lancet Psychiatry. 2017;4(2):146–58. doi: 10.1016/S2215-0366(16)30263-2 [DOI] [PubMed] [Google Scholar]
  • 60.Why is depression more prevalent in women? Available from: https://psycnet.apa.org/record/2015-41955-001. Accessed 2024 June 17.
  • 61.Diagnostic and statistical manual of mental disorders: DSM-5TM. 5th ed. Arlington, VA, US: American Psychiatric Publishing, Inc.; 2013. [Google Scholar]
  • 62.Sardinha L, Maheu-Giroux M, Stöckl H, Meyer SR, García-Moreno C. Global, regional, and national prevalence estimates of physical or sexual, or both, intimate partner violence against women in 2018. Lancet. 2022;399(10327):803–13. doi: 10.1016/S0140-6736(21)02664-7 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 63.Chen Y-Y, Subramanian SV, Acevedo-Garcia D, Kawachi I. Women’s status and depressive symptoms: a multilevel analysis. Soc Sci Med. 2005;60(1):49–60. doi: 10.1016/j.socscimed.2004.04.030 [DOI] [PubMed] [Google Scholar]
  • 64.Van de Velde S, Huijts T, Bracke P, Bambra C. Macro-level gender equality and depression in men and women in Europe. Sociol Health Illn. 2013;35(5):682–98. doi: 10.1111/j.1467-9566.2012.01521.x [DOI] [PubMed] [Google Scholar]
  • 65.Frech A, Williams K. Depression and the psychological benefits of entering marriage. J Health Soc Behav. 2007;48(2):149–63. doi: 10.1177/002214650704800204 [DOI] [PubMed] [Google Scholar]
  • 66.Umberson D, Montez JK. Social relationships and health: a flashpoint for health policy. J Health Soc Behav. 2010;51 Suppl(Suppl):S54-66. doi: 10.1177/0022146510383501 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 67.The Effects of Marriage on Health: A Synthesis of Recent Research Evidence. Research Brief. In: ASPE. 2007. https://aspe.hhs.gov/reports/effects-marriage-health-synthesis-recent-research-evidence-research-brief. Accessed 18 Jun 2024 [Google Scholar]
  • 68.Huang X. The politics of social welfare reform in urban China: Social welfare preferences and reform policies. J Chin Polit Sci. 2012;18(1):61–85. doi: 10.1007/s11366-012-9227-x [DOI] [Google Scholar]
  • 69.Wang X, Wu S, Song Q, Tse L-A, Yu ITS, Wong T-W, et al. Occupational health and safety challenges in China--focusing on township-village enterprises. Arch Environ Occup Health. 2011;66(1):3–11. doi: 10.1080/19338244.2010.486424 [DOI] [PubMed] [Google Scholar]
  • 70.Mei S, Qin Z, Yang Y, Gao T, Ren H, Hu Y, et al. Influence of life satisfaction on quality of life: mediating roles of depression and anxiety among cardiovascular disease patients. Clin Nurs Res. 2021;30(2):215–24. doi: 10.1177/1054773820947984 [DOI] [PubMed] [Google Scholar]
  • 71.Chen Y, Hicks A, While AE. Quality of life and related factors: a questionnaire survey of older people living alone in Mainland China. Qual Life Res. 2014;23(5):1593–602. doi: 10.1007/s11136-013-0587-2 [DOI] [PubMed] [Google Scholar]
  • 72.Zhong W, Wang F, Chi L, Yang X, Yang Y, Wang Z. Association between sleep duration and depression among the elderly population in China. Exp Aging Res. 2022;48(4):387–99. doi: 10.1080/0361073X.2021.2008755 [DOI] [PubMed] [Google Scholar]
  • 73.Ouyang P, Sun W. Depression and sleep duration: findings from middle-aged and elderly people in China. Public Health. 2019;166:148–54. doi: 10.1016/j.puhe.2018.10.007 [DOI] [PubMed] [Google Scholar]
  • 74.Sun Y, Shi L, Bao Y, Sun Y, Shi J, Lu L. The bidirectional relationship between sleep duration and depression in community-dwelling middle-aged and elderly individuals: evidence from a longitudinal study. Sleep Med. 2018;52:221–9. doi: 10.1016/j.sleep.2018.03.011 [DOI] [PubMed] [Google Scholar]
  • 75.Lyubomirsky S, King L, Diener E. The benefits of frequent positive affect: does happiness lead to success?. Psychol Bull. 2005;131(6):803–55. doi: 10.1037/0033-2909.131.6.803 [DOI] [PubMed] [Google Scholar]
  • 76.Miao H, Sun H, He X, Zhang Z, Nie Q, Guo C. Perceived social support and life satisfaction among young Chinese adolescents: the mediating effect of psychological Suzhi and its components. Curr Psychol. 2020;40(12):6164–74. doi: 10.1007/s12144-020-01130-3 [DOI] [Google Scholar]
  • 77.Demiral Y, Ihle T, Rose U, Conway PM, Burr H. Precarious work as risk factor for 5-year increase in depressive symptoms. Int J Environ Res Public Health. 2022;19(6):3175. doi: 10.3390/ijerph19063175 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 78.Oddo VM, Zhuang CC, Andrea SB, Eisenberg-Guyot J, Peckham T, Jacoby D, et al. Changes in precarious employment in the United States: a longitudinal analysis. Scand J Work Environ Health. 2021;47(3):171–80. doi: 10.5271/sjweh.3939 [DOI] [PMC free article] [PubMed] [Google Scholar]

Decision Letter 0

Wenbin Du

PONE-D-24-33298Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediatorPLOS ONE

Dear Dr. Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

I hope this message finds you well. Thank you for submitting your manuscript, "Can Working in Agriculture Have a Favorable Effect on Depressive Symptoms? Life Satisfaction as a Mediator," to PLOS ONE. I have carefully reviewed your work along with the comments provided by our esteemed reviewers. While all three reviewers have recommended minor revisions, upon careful consideration of their feedback and a thorough evaluation of your manuscript, I believe that a more comprehensive set of changes—what we typically categorize as a major revision—would significantly enhance the quality and clarity of your paper.

Please submit your revised manuscript by Dec 19 2024 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Wenbin Du

Academic Editor

PLOS ONE

Journal requirements: 

1. When submitting your revision, we need you to address these additional requirements.

Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at 

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and 

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. Thank you for stating in your Funding Statement: 

Funding

This research is supported by Key Laboratory of Philosophy and Social Sciences of Colleges and Universities in Guangdong Province (2023WSYS005, 2023WSYS007), the 13th Five-Year Plan of Guangdong Province for Philosophy and Social Sciences (GD20XGL42).

Please provide an amended statement that declares *all* the funding or sources of support (whether external or internal to your organization) received during this study, as detailed online in our guide for authors at http://journals.plos.org/plosone/s/submit-now.  Please also include the statement “There was no additional external funding received for this study.” in your updated Funding Statement. 

Please include your amended Funding Statement within your cover letter. We will change the online submission form on your behalf.

3. In the online submission form, you indicated that your data is available only on request from a third party. Please note that your Data Availability Statement is currently missing the name of the third party contact or institution. Please update your statement with the missing information. 

4. PLOS requires an ORCID iD for the corresponding author in Editorial Manager on papers submitted after December 6th, 2016. Please ensure that you have an ORCID iD and that it is validated in Editorial Manager. To do this, go to ‘Update my Information’ (in the upper left-hand corner of the main menu), and click on the Fetch/Validate link next to the ORCID field. This will take you to the ORCID site and allow you to create a new iD or authenticate a pre-existing iD in Editorial Manager.

5. Your ethics statement should only appear in the Methods section of your manuscript. If your ethics statement is written in any section besides the Methods, please delete it from any other section. 

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1:  1. Add 'China' to the keywords to improve the searches of the article when published on online databases.

2. In the introduction, it is important to expose the research gaps by reviewing related studies in the Chinese context especially. While the global stretch of this have been done, only a study referencing China has been cited though there are a lot of studies in China that have investigated the correlation between job(s) and depression with life satisfaction playing a mediating role.

3. It's important to reference some of the studies that have validated the CES-D scale with much success, pointing to it as reliable and as such efficacious for such a study.

4. In the selection of the covariates, do well to reference the previous studies that were consulted and justify the covariates that were settled on for the study.

5. In the discussion, the proposition put forward which is based on the significant results attained after running rigorous analysis, which are lower life satisfaction and social activity participation as major risk factors for the development of depression among agricultural workers need to be discussed from the perspective of other empirical studies. Which existing studies on work-related depression have recorded similar results? What might probably be the underlying causative factors for recording such results? Discussion of these questions must be expanded in the discussion section. This was beautifully done in the discussion on the high risk of depression recorded among self-employed agricultural workers.

6. The limitations must be moved from the discussion to the concluding section. Also, after summarizing the study's purpose, it is important to reiterate the key results and draw valid conclusions from them based on the underlying research questions and hypotheses set. This has not been done.

Reviewer #2:  The study “Can working in agriculture have a favorable effect on depressive symptoms? Life satisfaction as a mediator” investigates the impact of agricultural work on depressive symptoms and whether life satisfaction mediates this relationship. The study concluded that self-employed workers in agriculture have a higher risk of developing depressive states and present a high risk of developing depression. This is a relevant finding for research related to depressive disorders. However, there are areas that require clarification and significant improvements, particularly regarding the background, research design development, data analysis, and alignment of the research questions with the results. Addressing these points would strengthen the overall impact and credibility of the work. Notably, the manuscript is generally well-written, and the authors should be able to address my points through a revision. My comments and suggestions for improvement are described below.

Introduction

2nd paragraph:

In the excerpt: "...but there is still no research focusing on the association between job type and depression…"

There is an inconsistency because the two references below associate depression and job type:

1-) Kim S, Kwon M, Seo K. Factors Influencing the Health-Related Quality of Life of Workers According to the Type of Work. Healthcare (Basel). 2022 Oct 18;10(10):2066. doi: 10.3390/healthcare10102066;

2-) Kang W, Park WJ, Jang KH, Lim HM, Ann JS, Cho SH, Moon JD. Comparison of anxiety and depression status between office and manufacturing job employees in a large manufacturing company: a cross sectional study. Ann Occup Environ Med. 2016 Sep 15;28:47. doi: 10.1186/s40557-016-0134-z.

2nd and 3rd paragraphs: In the excerpts below:

“This study will then look at different types of work - agricultural and non-agricultural - and explore the association with depression, and further analyze subgroups of the population according to whether they are self-employed or employed".

“Therefore, in this study, it is necessary to investigate whether the type of jobs has a correlation with life satisfaction and to include life satisfaction in the model as a mediating variable to investigate whether the type of jobs acts on the depressive state through life satisfaction”.

I suggest focusing the research objectives in a single paragraph, preferably in the last paragraph of the Introduction.

3nd paragraph: In the excerpt: " ...It has been proven that there is an association between life satisfaction and depression in middle-aged and elderly people."

It is not clear what type of association exists between the two mentioned variables.

Method

Research design

I suggest specifying the method used for the sample calculation.

Study population

I suggest reporting the exclusion criteria applied to the study.

Measurement Tools

Life satisfaction

In the excerpt: “Life satisfaction was assessed at baseline by asking participants: How satisfied are you with your life in general?"

Did the participants receive any information about what life satisfaction is and/or some more objective criteria to assist in their self-analysis?

I believe that a subjective question may lead participants to potential interpretation biases, making it necessary to establish criteria for them to respond more accurately.

Methods of statistical analysis

It is not clear what covariates were included in the correlation analysis.

Result/Discussion

I suggest reporting the influence of working hours and its relationship with depression and life satisfaction of agricultural workers.

Discussion

4nd paragraph:

In the excerpt: “There are many factors that contribute to the high prevalence of depressive states in the female population, including a combination of biological, psychological, and sociocultural factors. Biologically, women are more sensitive to hormonal fluctuations associated with the reproductive cycle, such as hormonal fluctuations (menstrual cycle, postpartum, and menopause) that can affect mood thus leading to the occurrence of premenstrual irritability disorder and postpartum depressive states, among others[32 –34]; psychology points out that women are more prone to recurring thoughts of negativity[32], which may be associated with an increased risk of depressive states; and in the process of growing up, the Gender roles and societal expectations place additional stress on women, with sexual violence [35], sexual abuse [33], interpersonal violence, stress exposure and stress susceptibility [33] at the micro level, and gender inequality[36, 37] at the macro level, all of which increase the risk for the development of depressive states in women.”

What is the relationship between the mentioned factors and depression associated with agricultural work?

Conclusion

I suggest reporting the relevance of the research for public health policies in China. In light of the study's results, what can be done to improve the quality of life for agricultural workers?

Reviewer #3:  1. The study addresses an important and understudied area: the relationship between agricultural work and depressive symptoms. However, the use of a single-item measure for life satisfaction, while common, may not capture the full complexity of the construct. The study doesn't fully explore potential mechanisms underlying the relationship between agricultural work and depressive symptoms.

2. the article presents a well-structured study with a solid methodology and employing appropriate statistical techniques for the research questions. The use of propensity score matching to balance the agricultural and non-agricultural groups is a strength. The results are computed using appropriate statistical techniques, including logistic regression analysis to identify protective factors associated with life satisfaction and depressive symptoms. The use of odds ratios (OR) with 95% confidence intervals (CI) provides a clear understanding of the strength and significance of the associations. Additionally, mediation analysis is conducted to explore the role of life satisfaction in the relationship between job type and depressive symptoms, which is a robust approach to understanding causal pathways. The sensitivity analysis adds another layer of reliability to the findings, indicating that the mediating effects are not overly influenced by confounding variables. Overall, the statistical methods employed are appropriate for the research questions posed. However, there are some potential drawbacks:

• The dichotomization of the CES-D scores for logistic regression may lead to loss of information.

• The study doesn't address potential selection bias - people with certain characteristics may be more likely to engage in agricultural work.

3. The finding that agricultural workers have higher depressive symptom scores is surprising and doesn't align with some existing literature on the potential mental health benefits of nature-based activities. However, this could be due to other factors associated with agricultural work in China, such as economic stress or physical strain. The mediating role of life satisfaction is consistent with previous research on well-being and mental health. The subgroup analyses provide interesting insights into how the relationships differ across various populations, which adds nuance to the overall findings.

4. There are numerous grammatical and typographical errors throughout the text, which should be carefully edited.

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #1: Yes:  Dickson Adom

Reviewer #2: Yes:  Débora Regina de Aguiar

Reviewer #3: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/ . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org . Please note that Supporting Information files do not need this step.

PLoS One. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912.r002

Author response to Decision Letter 1


28 Nov 2024

Dear Reviewer 1,

Thank you very much for your comments. I have read your comments carefully and made a modification to the original manuscript. The revised part was marked in yellow based on your comments. The following are my responses to your questions.

Reviewer #1: 1. Add 'China' to the keywords to improve the searches of the article when published on online databases.

Our response: Thank you for your kind comment and advise, we have revised and added the key word “China” into our key words.(It can be reviewed in the Key Words part in our revised manuscript in yellow):

“Key Words: Agriculture work; Depressive symptoms; Life satisfaction; CHARLS; China”

2. In the introduction, it is important to expose the research gaps by reviewing related studies in the Chinese context especially. While the global stretch of this have been done, only a study referencing China has been cited though there are a lot of studies in China that have investigated the correlation between job(s) and depression with life satisfaction playing a mediating role.

Our response: Thank you for your valuable feedback. Our research was conducted within the context of China, and it was an oversight on our part not to cite more studies specific to China. We have now included several references that examine the relationship between work and depressive symptoms within the Chinese context, highlighting the mediating role of life satisfaction,as follows(At Line 62-64 in revised manuscript):

“Research has shown that work is closely related to the development of depression[4], which can be subdivided into work pressure[5], work hours[6, 7] , work environment[8, 9],work support[10],subjective social status[11]and so on,...”

3. It's important to reference some of the studies that have validated the CES-D scale with much success, pointing to it as reliable and as such efficacious for such a study.

Our response: Thank you for your valuable comment, it is our flaw to make you confuse about our validity of the CES-D scale. We have cited previous researches about the scale to evident its validity and reliability, which is as follows(At Line 126 in revised manuscript):

“... ,which is now a widely used and proven valid and reliable common indicator of depression[19, 20].”

4. In the selection of the covariates, do well to reference the previous studies that were consulted and justify the covariates that were settled on for the study.

Our response: Thank you for your valuable comment.We have cited previous studies to demonstrate the relationships between each covariate and the dependent variable (depressive symptoms), thereby supporting the rationale for our choice of covariates.

5. In the discussion, the proposition put forward which is based on the significant results attained after running rigorous analysis, which are lower life satisfaction and social activity participation as major risk factors for the development of depression among agricultural workers need to be discussed from the perspective of other empirical studies. Which existing studies on work-related depression have recorded similar results? What might probably be the underlying causative factors for recording such results? Discussion of these questions must be expanded in the discussion section. This was beautifully done in the discussion on the high risk of depression recorded among self-employed agricultural workers.

Our response: Thank you for your valuable comment.We have expanded our discussion and thoroughly restructured the logical flow in Discussion section,as follows(At Line 329-344 in revised manuscript):

“The reasons for low life satisfaction among agricultural workers are complex, primarily influenced by the following factors:(1) Economic Pressure[41]: Agricultural workers’ incomes are generally less stable compared to those in non-agricultural occupations, as they are highly susceptible to weather changes, market fluctuations, and other external factors. The seasonal and uncertain nature of agricultural income can impose substantial financial stress, leading to lower life satisfaction.(2) Occupational Health Risks[42]: Agricultural workers face high occupational health risks, including long working hours, exposure to adverse weather conditions, and pesticide exposure mentioned earlier. These health risks negatively impact both physical and mental well-being, reducing overall life satisfaction.(3) Social Isolation and Lack of Social Support[41]: Agricultural workers are often geographically isolated due to the nature of their work, resulting in limited social interactions and relationships. This isolation contributes to lower levels of social participation. The lack of social support and limited opportunities for interaction may lead to feelings of loneliness and depressive symptoms, further impacting life satisfaction.(4) Mental Health Issues and Cultural Stigma[42]: In rural areas, mental health issues are still subject to cultural stigma, which makes it difficult for agricultural workers experiencing mental health challenges to seek help. The lack of mental health support resources can exacerbate these issues, leading to further declines in life satisfaction.”

6. The limitations must be moved from the discussion to the concluding section. Also, after summarizing the study's purpose, it is important to reiterate the key results and draw valid conclusions from them based on the underlying research questions and hypotheses set. This has not been done.

Our response�Thank you for your valuable advise and comment, we have adjusted the narration order, revised the language and moved it to the conclusion section. For helping you better review our revised part, the revised part is at conclusion section Line 413-420 of revised manuscript.

Best Regards,

Yuan Yao Chen

yuanyaochen0401@163.com

Dear Reviewer 2,

Thank you very much for your comments and appreciation. We have read your comments carefully and made a modification to the original manuscript. The revised part was marked in yellow based on your comments. The following are my responses to your questions.

Reviewer #2: The study “Can working in agriculture have a favorable effect on depressive symptoms? Life satisfaction as a mediator” investigates the impact of agricultural work on depressive symptoms and whether life satisfaction mediates this relationship. The study concluded that self-employed workers in agriculture have a higher risk of developing depressive states and present a high risk of developing depression. This is a relevant finding for research related to depressive disorders. However, there are areas that require clarification and significant improvements, particularly regarding the background, research design development, data analysis, and alignment of the research questions with the results. Addressing these points would strengthen the overall impact and credibility of the work. Notably, the manuscript is generally well-written, and the authors should be able to address my points through a revision. My comments and suggestions for improvement are described below.

Our response�Thank you for your appreciation, valuable comments and suggestions on our research, we have carefully read and responded to your comments as following responses. We have fixed and revised most of the problems you mentioned and still tried to improve our future study based your comments.

Introduction

1.2nd paragraph:

In the excerpt: "...but there is still no research focusing on the association between job type and depression…"

There is an inconsistency because the two references below associate depression and job type:

Our response�Inappropriate wording in our article was an oversight on our part. Our paper examines the relationship between two types of jobs—agricultural and non-agricultural work which we have made an explanation at Method—and depressive symptoms. However, the definitions of “job type” in the two references below differ from ours. We are very grateful for your feedback on this matter and have made the following revisions accordingly (At Line 65 in revised manuscript):

“but there is still no research focusing on the association between job type—agricultural and non-agricultural— and depression.”

2.“This study will then look at different types of work - agricultural and non-agricultural - and explore the association with depression, and further analyze subgroups of the population according to whether they are self-employed or employed".

“Therefore, in this study, it is necessary to investigate whether the type of jobs has a correlation with life satisfaction and to include life satisfaction in the model as a mediating variable to investigate whether the type of jobs acts on the depressive state through life satisfaction”.I suggest focusing the research objectives in a single paragraph, preferably in the last paragraph of the Introduction.

3nd paragraph: In the excerpt: " ...It has been proven that there is an association between life satisfaction and depression in middle-aged and elderly people."

It is not clear what type of association exists between the two mentioned variables.

Our response�Thank you for your valuable comment.

(1)We have made revisions and consolidated the study objectives in the final paragraph of the introduction, as follows(At Line 79-83 in revised manuscript):

“This study will first examine the association between different types of work and depressive symptoms. Further, it will analyze population subgroups based on work state—self-employed versus employed—in order to provide more targeted public health recommendations. Additionally, life satisfaction will be incorporated into the model as a mediating variable to explore whether job types influence depressive symptoms through the pathway of life satisfaction.”

(2)The two referenced studies[14,15] reveal that previous research has established a correlation between depressive symptoms and life satisfaction. Reference [14] indicates that life satisfaction can serve as a potential indicator of depression, although it may be influenced by gender differences. Reference [15] highlights a reciprocal and enduring relationship between life satisfaction and depression.

Method

2.Research design

I suggest specifying the method used for the sample calculation.

Our response�Thank you for your valuable comment, it is our flaw that the method used for the sample calculation is not explained enough. Firstly, the data we use for analysis in this article comes from the public database CHARLS,which are covered in detail in the Method_ Research design section.For the convenience of your review, we have modified the flow chart (Fig.1) to make you more clear about the method used in our sample calculation. Additionally,We have added our methodology for data analysis as follows(At Line 173 in revised manuscript):

“The above statistical analysis is based on R-4.3.1.”

3.Study population

I suggest reporting the exclusion criteria applied to the study.

Our response�Thank you for your valuable comment.In our study, the criteria for inclusion of subjects at baseline were: subjects who contained a record of jobs type , assessment of depressive states, assessment of life satisfaction, age, gender, type of residence, work states, marital states, socially engaged activity score, smoking states, drinking states, sleep duration, prior work states in CHARLS 2015. Meanwhile, we have excluded subjects that have missing values in the variables given upon.We have explained this in the Methods_Study population section of the manuscript.For the convenience of your review, we have modified the flow chart (Fig.1) to make you more clear about our exclusion criteria.

4.Measurement Tools

Life satisfaction

In the excerpt: “Life satisfaction was assessed at baseline by asking participants: How satisfied are you with your life in general?"

Did the participants receive any information about what life satisfaction is and/or some more objective criteria to assist in their self-analysis?

I believe that a subjective question may lead participants to potential interpretation biases, making it necessary to establish criteria for them to respond more accurately.

Our response�Thank you for your valuable comment.The data we use for analysis in this article comes from the public database CHARLS.There is a question "How satisfied are you with your life in general?" included in the questionnaire of CHARLS,which have been proved that strongly correlates with the validated 5-item Satisfaction with Life Scale (SWLS).We have cited the previous research[21] to explain this correlation.

5.Methods of statistical analysis

It is not clear what covariates were included in the correlation analysis.

Our response�Thank you for your valuable comment.The covariates included in our study cover demographic characteristics such as age, gender, type of residential address and marital states, as well as factors that have been validated by research to be highly correlated with depressive states, such as social activity participation, smoking states, drinking states.We have illustrated in Method_Selection of covariates and shown in Fig.3.

Result/Discussion

6.I suggest reporting the influence of working hours and its relationship with depression and life satisfaction of agricultural workers.

Our response�Thank you for your valuable comment.Certainly, as you mentioned, this is a very worthwhile area of study. However, because we used a public database, the questionnaire design in this database did not include questions regarding ‘working hours.’ Consequently, we lack a data source on working hours, which limits our ability in this study to explore the effects of working hours and their relationship with depressive symptoms and life satisfaction among agricultural workers. We are eager and will strive to incorporate this direction into future research as we undertake independent studies.

Discussion

7.4nd paragraph:

In the excerpt: “There are many factors that contribute to the high prevalence of depressive states in the female population, including a combination of biological, psychological, and sociocultural factors. Biologically, women are more sensitive to hormonal fluctuations associated with the reproductive cycle, such as hormonal fluctuations (menstrual cycle, postpartum, and menopause) that can affect mood thus leading to the occurrence of premenstrual irritability disorder and postpartum depressive states, among others[32 –34]; psychology points out that women are more prone to recurring thoughts of negativity[32], which may be associated with an increased risk of depressive states; and in the process of growing up, the Gender roles and societal expectations place additional stress on women, with sexual violence [35], sexual abuse [33], interpersonal violence, stress exposure and stress susceptibility [33] at the micro level, and gender inequality[36, 37] at the macro level, all of which increase the risk for the development of depressive states in women.”

What is the relationship between the mentioned factors and depression associated with agricultural work?

Our response: Thank you for your comment and valuable question, it is our flaw not to narrative clearly about the association between Agricultural labor related depression and female factor. We have already added extended discussion and fully reorganized our logic in the discussion part. To help you better review our revised part, it i as follows:

“The reasons for low life satisfaction among agricultural workers are complex, primarily influenced by the following factors:(1) Economic Pressure[41]: Agricultural workers’ incomes are generally less stable compared to those in non-agricultural occupations, as they are highly susceptible to weather changes, market fluctuations, and other external factors. The seasonal and uncertain nature of agricultural income can impose substantial financial stress, leading to lower life satisfaction.(2) Occupational Health Risks[42]: Agricultural workers face high occupational health risks, including long working hours, exposure to adverse weather conditions, and pesticide exposure mentioned earlier. These health risks negatively impact both physical and mental well-being, reducing overall life s

Attachment

Submitted filename: Reply to editor, reviewer1, reviewer2 and reviewer3.docx

pone.0325912.s002.docx (46.5KB, docx)

Decision Letter 1

Zahra Lorigooini

PONE-D-24-33298R1Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediatorPLOS ONE

Dear Dr. Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by May 10 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Zahra Lorigooini

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #3: (No Response)

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #3: Partly

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #3: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #3: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #3: No

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1:  Thanks for revising the manuscript based on my earlier comments. The rigor of the manuscript has been enhanced.

Reviewer #3:  The literature review is reasonably comprehensive, summarizing relevant studies related to depression, work-related factors, and life satisfaction. However, it could benefit from the incorporation of more recent studies and contrasting findings to provide a wider context. While the identification of gaps in current research is commendable, particularly in relation to job types and depressive symptoms, the paper would be fortified by clearly detailing how the current study directly addresses these identified gaps.

The methodology employed in the study is appropriate for the research questions posed, utilizing a large and representative sample from the China Health and Retirement Longitudinal Study (CHARLS). This approach enhances the generalizability of the findings. The description of the methodology is adequately detailed, covering aspects such as data collection, participant selection, measurement tools, and statistical analyses. However, a more explicit discussion regarding the handling of potential confounders would improve the overall clarity of the methodology section.

The results are presented in a clear and logical manner, with a commendable use of statistics to support the findings. The application of logistic regression and mediation analyses is well-documented. The article makes effective use of tables and figures to present data; however, clearer captions summarizing the key findings for each table and figure would enhance reader comprehension.

The discussion effectively ties back to the research question and objectives, interpreting the results in the context of the existing literature. While the limitations of the study are acknowledged, including its cross-sectional nature and reliance on self-reported measures, a more thorough exploration of the implications of these limitations would enrich the discussion.

The article is generally well-written and clear, although some sections could benefit from reduced jargon and more straightforward language to improve accessibility for a broader audience. The logical structure of the article facilitates a coherent flow from the introduction, through methodology and results, to discussion and conclusion.

The study adheres to ethical standards as evidenced by approval from the Peking University Ethics Committee and informed consent obtained from participants. However, potential conflicts of interest are not explicitly addressed, which should be included to enhance transparency.

The references cited in the article are relevant, although some may be outdated. Incorporating more recent studies could bolster the literature supporting the research. The article appears to follow a consistent citation style; however, a final review is advisable to ensure completeness and accuracy.

Recommendations

To enhance the quality of the article prior to publication, I recommend the following revisions:

• Incorporate more recent studies and contrasting findings to broaden the context and strengthen the literature base.

• Provide a more in-depth discussion of the implications of the study’s limitations, particularly regarding the cross-sectional design and reliance on self-reported measures.

• Include a section addressing potential conflicts of interest to improve transparency and adhere to ethical standards.

• Simplify some sections of the text to reduce jargon and make the article more accessible to a wider audience.

• Add clearer captions that summarize the key findings of each table and figure to enhance reader comprehension.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #1: Yes:  Dickson Adom

Reviewer #3: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/ . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org . Please note that Supporting Information files do not need this step.

PLoS One. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912.r004

Author response to Decision Letter 2


2 Apr 2025

Dear Editor�

Thank you for your kind comment and rapid arrangement on our revision, we have carefully read and replied to every comment made by 2 reviewers on our manuscript. Please review our revised manuscript and response letter.

Hope everything smooth.

Best Regards,

Yuan Yao Chen

yuanyaochen0401@163.com

Reviewer #1: Thanks for revising the manuscript based on my earlier comments. The rigor of the manuscript has been enhanced.

Dear Reviewer 1,

Thank you very much for your comments. We really appreciate your approval and recognition of our revised work.

Hope everything smooth.

Best Regards,

Yuan Yao Chen

yuanyaochen0401@163.com

Reviewer #3: The literature review is reasonably comprehensive, summarizing relevant studies related to depression, work-related factors, and life satisfaction. However, it could benefit from the incorporation of more recent studies and contrasting findings to provide a wider context. While the identification of gaps in current research is commendable, particularly in relation to job types and depressive symptoms, the paper would be fortified by clearly detailing how the current study directly addresses these identified gaps.

The methodology employed in the study is appropriate for the research questions posed, utilizing a large and representative sample from the China Health and Retirement Longitudinal Study (CHARLS). This approach enhances the generalizability of the findings. The description of the methodology is adequately detailed, covering aspects such as data collection, participant selection, measurement tools, and statistical analyses. However, a more explicit discussion regarding the handling of potential confounders would improve the overall clarity of the methodology section.

The results are presented in a clear and logical manner, with a commendable use of statistics to support the findings. The application of logistic regression and mediation analyses is well-documented. The article makes effective use of tables and figures to present data; however, clearer captions summarizing the key findings for each table and figure would enhance reader comprehension.

The discussion effectively ties back to the research question and objectives, interpreting the results in the context of the existing literature. While the limitations of the study are acknowledged, including its cross-sectional nature and reliance on self-reported measures, a more thorough exploration of the implications of these limitations would enrich the discussion.

The article is generally well-written and clear, although some sections could benefit from reduced jargon and more straightforward language to improve accessibility for a broader audience. The logical structure of the article facilitates a coherent flow from the introduction, through methodology and results, to discussion and conclusion.

The study adheres to ethical standards as evidenced by approval from the Peking University Ethics Committee and informed consent obtained from participants. However, potential conflicts of interest are not explicitly addressed, which should be included to enhance transparency.

The references cited in the article are relevant, although some may be outdated. Incorporating more recent studies could bolster the literature supporting the research. The article appears to follow a consistent citation style; however, a final review is advisable to ensure completeness and accuracy.

Dear Reviewer 3,

Thank you very much for your comments and approval on our manuscript! We have read your comments carefully and made a modification to the original manuscript. The revised part was marked in yellow based on your comments. The following are my responses to your comments.

Recommendations

To enhance the quality of the article prior to publication, I recommend the following revisions:

• Incorporate more recent studies and contrasting findings to broaden the context and strengthen the literature base.

• Provide a more in-depth discussion of the implications of the study’s limitations, particularly regarding the cross-sectional design and reliance on self-reported measures.

• Include a section addressing potential conflicts of interest to improve transparency and adhere to ethical standards.

• Simplify some sections of the text to reduce jargon and make the article more accessible to a wider audience.

• Add clearer captions that summarize the key findings of each table and figure to enhance reader comprehension.

Reply to Reviewer3:

Thank you for your detailed and precious comments on our manuscript, we have carefully read your comments and made revise on the revised manuscript on yellow.

1.For incorporating more recent studies and contrasting findings to broaden the context, we have added new content to support our study background in Introduction Sections:

“While empirical research has demonstrated a significantly elevated prevalence of depressive symptoms among middle-aged and elderly populations residing in rural China compared to their urban counterparts[14]. This epidemiological phenomenon stands in stark contrast to the theoretical postulates of Horticultural Therapy, which advocate the mental health benefits derived from nature immersion. The findings reveal a complex mechanistic relationship between agrarian occupational environments and psychological well-being, indicating that mere proximity to natural elements fails to comprehensively elucidate the etiological pathways through which agricultural labor influences depressive states.”

2.For more in-depth discussion of the implications of the study’s limitations, we have added narration about the cross-sectional design and reliance on self-reported measures in the last part of Discussion Section on yellow.

3.We have complete our conflict of interest narration to improve transparency and adhere to ethical standards, which is as follows:

“The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.”

4.As for clearer captions, we have adjusted the title of Figure3-5 in order to better summarize the content of the figures. Moreover, we have optimized the narration of Result Sections to more concisely and accurately describe our result.

Best Regards,

Yuan Yao Chen

yuanyaochen0401@163.com

Attachment

Submitted filename: revised manuscript 3.0.docx

pone.0325912.s003.docx (146.3KB, docx)

Decision Letter 2

Zahra Lorigooini

PONE-D-24-33298R2Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediatorPLOS ONE

Dear Dr. Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by May 28 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Zahra Lorigooini

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #3: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #3: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #3: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #3: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #3: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #3: • The background is relatively up-to-date, mentioning key studies but could benefit from a broader range of literature around depression and agricultural work.

• The objectives are clearly stated, aiming to explore the association of agricultural work with depressive symptoms and the mediating role of life satisfaction.

• The study methods are sufficiently detailed for replication, including sampling and assessment tools used (CHARLS data, CES-D scale for depression).

• The statistical analyses appear appropriate, utilizing regression methods and sensitivity analyses to ensure robustness in findings.

• The methodology does attempt to address potential biases by including various covariates and controlling for other factors influencing mental health.

• Research was conducted in an ethical manner, approved by the relevant institutional review board.

• The findings are presented clearly, with appropriate statistical results discussed, though incorporating more visual aids could enhance clarity.

• Results align well with stated objectives, showing significant associations between agricultural work, depressive symptoms, and life satisfaction.

• While the initial analyses are thorough, sensitivity analyses and confidence intervals could enhance the validity of the results.

• The authors provide evidence supporting their claims, referencing statistical findings effectively.

• The discussion includes comparisons with existing literature, although a more in-depth exploration of how findings differ from or confirm previous studies could strengthen arguments.

• The alternative interpretations of findings considered are not considered. The authors could expand on alternative interpretations of their findings for a more balanced discussion.

• Conclusions logically follow the data and analyses presented.

• Limitations are acknowledged, but a deeper discussion on their implications could enhance the conclusion.

• The study does suggest avenues for future research, particularly in improving life satisfaction among agricultural workers.

• The manuscript is well-organized, though some sections could benefit from more concise presentations.

• The writing is generally clear and accessible, suitable for readers with a general background in health research.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #3: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/ . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org . Please note that Supporting Information files do not need this step.

PLoS One. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912.r006

Author response to Decision Letter 3


16 Apr 2025

Dear Editor,

Thank you for your kind decision to have a revision chance! We have thoroughly read any comments made by Reviewers and indeed replied. Thank you again!

Best regards,

Yuan Yao Chen

Reviewer #3: 

Dear Reviewer3,

It is our honor to have your approval of our revised manuscript. We really appreciate your precious comments and advice. Thank you again for your highly speak of our work.

• The background is relatively up-to-date, mentioning key studies but could benefit from a broader range of literature around depression and agricultural work.

• The objectives are clearly stated, aiming to explore the association of agricultural work with depressive symptoms and the mediating role of life satisfaction.

• The study methods are sufficiently detailed for replication, including sampling and assessment tools used (CHARLS data, CES-D scale for depression).

• The statistical analyses appear appropriate, utilizing regression methods and sensitivity analyses to ensure robustness in findings.

• The methodology does attempt to address potential biases by including various covariates and controlling for other factors influencing mental health.

• Research was conducted in an ethical manner, approved by the relevant institutional review board.

• The findings are presented clearly, with appropriate statistical results discussed, though incorporating more visual aids could enhance clarity.

• Results align well with stated objectives, showing significant associations between agricultural work, depressive symptoms, and life satisfaction.

• While the initial analyses are thorough, sensitivity analyses and confidence intervals could enhance the validity of the results.

• The authors provide evidence supporting their claims, referencing statistical findings effectively.

• The discussion includes comparisons with existing literature, although a more in-depth exploration of how findings differ from or confirm previous studies could strengthen arguments.

• The alternative interpretations of findings considered are not considered. The authors could expand on alternative interpretations of their findings for a more balanced discussion.

• Conclusions logically follow the data and analyses presented.

• Limitations are acknowledged, but a deeper discussion on their implications could enhance the conclusion.

• The study does suggest avenues for future research, particularly in improving life satisfaction among agricultural workers.

• The manuscript is well-organized, though some sections could benefit from more concise presentations.

• The writing is generally clear and accessible, suitable for readers with a general background in health research.

Reply: It is our honor to have your approval of our revised manuscript. We really appreciate your precious comments and advice. And we would continue our further study on Job diversities with mental health and try to figure out more reasonable explanation based on our current research, especially try to decline our limitations during collecting data and designing our study.

Thank you again for your highly speak of our work.

Best regards,

Yuan Yao Chen

Attachment

Submitted filename: renamed_36f54.docx

pone.0325912.s004.docx (11.9KB, docx)

Decision Letter 3

Zahra Lorigooini

PONE-D-24-33298R3Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediatorPLOS ONE

Dear Dr. Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Jun 06 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Zahra Lorigooini

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #3: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #3: Partly

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #3: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #3: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #3: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #3: The study addresses a critical issue and Utilizing the CHARLS 2015 dataset (n=6,856) enhances statistical power and generalizability to China’s middle-aged and elderly population. The use of CES-D for depressive symptoms and SWLS for life satisfaction strengthens the reliability of the outcomes. Inclusion of demographic, behavioral, and social factors (e.g., age, gender, social activity) reduces confounding bias. Mediation analysis via Baron and Kenny’s approach and Sobel test appropriately tests the hypothesized pathways.

However, consider the following concern to address them Limits causal inference. The observed associations could reflect reverse causality (e.g., depressive individuals may self-select into agricultural work).

1. Excluding 11,270 participants due to missing values risks selection bias. Multiple imputation or sensitivity analyses are needed to validate findings.

2. Dichotomizing jobs into "agricultural" vs. "non-agricultural" ignores heterogeneity within agricultural work (e.g., subsistence vs. commercial farming). Clarify contradictions (e.g., job type’s non-significance in logistic regression vs. abstract’s emphasis on self-employed risk).

3. Reliance on one question ("How satisfied are you with your life?") may lack depth compared to multi-item scales like SWLS.

4. Life satisfaction mediated only 5.13% of the total effect, questioning its practical significance. Other mediators (e.g., income stability, social support) were overlooked.

5. Self-employed agricultural workers showed more protective factors, conflicting with literature on self-employment stressors. The rationale for this is underexplored.

6. The 2015 dataset may not reflect recent socioeconomic changes in rural China (e.g., digitalization, policy shifts).

7. Inconsistent table/figure numbering, (e.g., Fig. 3–7 not provided), and unclear reporting of propensity score matching methodology. Provide full details on propensity score matching (variables, method, balance diagnostics).

Highlight them in limitation and recommend them for future consideration.

1. Use longitudinal data to establish temporal relationships.

2. Apply multiple imputation for missing data and report sensitivity analyses.

3. Refine job type categorization (e.g., differentiate farming practices, land ownership).

4. Consider alternative regression models (e.g., Poisson) for common outcomes to avoid overestimating odds ratios.

5. Explore additional mediators (e.g., economic stability, pesticide exposure) to explain the weak mediation effect of life satisfaction.

6. Include all figures/tables referenced (e.g., correlation matrix, mediation model).

7. Address any ethical and temporal relevance related to mental health.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #3: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/ . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org . Please note that Supporting Information files do not need this step.

PLoS One. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912.r008

Author response to Decision Letter 4


23 Apr 2025

Dear Reviewer3,

Thank you for your detailed comments and sincere help to help us improve our further research quality and enhance our narration of our limitations. We are sorry that due to the structure our original data set and observational study, it is hard to make deeper revision on the current study, however, we would continue to make our future research more complete under your guidance.

The study addresses a critical issue and Utilizing the CHARLS 2015 dataset (n=6,856) enhances statistical power and generalizability to China’s middle-aged and elderly population. The use of CES-D for depressive symptoms and SWLS for life satisfaction strengthens the reliability of the outcomes. Inclusion of demographic, behavioral, and social factors (e.g., age, gender, social activity) reduces confounding bias. Mediation analysis via Baron and Kenny’s approach and Sobel test appropriately tests the hypothesized pathways.

However, consider the following concern to address them Limits causal inference. The observed associations could reflect reverse causality (e.g., depressive individuals may self-select into agricultural work).

1. Excluding 11,270 participants due to missing values risks selection bias. Multiple imputation or sensitivity analyses are needed to validate findings.

2. Dichotomizing jobs into "agricultural" vs. "non-agricultural" ignores heterogeneity within agricultural work (e.g., subsistence vs. commercial farming). Clarify contradictions (e.g., job type’s non-significance in logistic regression vs. abstract’s emphasis on self-employed risk).

3. Reliance on one question ("How satisfied are you with your life?") may lack depth compared to multi-item scales like SWLS.

4. Life satisfaction mediated only 5.13% of the total effect, questioning its practical significance. Other mediators (e.g., income stability, social support) were overlooked.

5. Self-employed agricultural workers showed more protective factors, conflicting with literature on self-employment stressors. The rationale for this is underexplored.

6. The 2015 dataset may not reflect recent socioeconomic changes in rural China (e.g., digitalization, policy shifts).

7. Inconsistent table/figure numbering, (e.g., Fig. 3–7 not provided), and unclear reporting of propensity score matching methodology. Provide full details on propensity score matching (variables, method, balance diagnostics).

Highlight them in limitation and recommend them for future consideration.

1. Use longitudinal data to establish temporal relationships.

2. Apply multiple imputation for missing data and report sensitivity analyses.

3. Refine job type categorization (e.g., differentiate farming practices, land ownership).

4. Consider alternative regression models (e.g., Poisson) for common outcomes to avoid overestimating odds ratios.

5. Explore additional mediators (e.g., economic stability, pesticide exposure) to explain the weak mediation effect of life satisfaction.

6. Include all figures/tables referenced (e.g., correlation matrix, mediation model).

7. Address any ethical and temporal relevance related to mental health.

Authors’ Reply: Thank you for your kind comment! We have added several advice and comment into our Section Discussion and Limitations, which were highlighted in yellow in our revised manuscript. Moreover, we have checked and revised the miss cited table&figure in the manuscript, cited them in the right order and reapplied them into the system. The revised limitations were as follows:

“This study has several limitations. First, it is based on cross-sectional data from the 2015 China Health and Retirement Longitudinal Study (CHARLS), which limits the ability to establish a clear temporal sequence between agricultural employment and depressive symptoms. Consequently, reverse causality cannot be ruled out; for instance, individuals with depression may be more inclined to engage in agricultural work.Longitudinal designs are essential for understanding the temporal relationship between job type and mental health outcomes. Existing longitudinal investigations have illuminated that the depressive symptoms is not static; rather, it can fluctuate over time, which cross-sectional studies may not adequately represent. Longitudinal research highlights the dynamic relationship between job type and mental health, with studies showing that unstable employment can lead to increased depressive symptoms over time[77] and work-related stressors can exacerbate mental health issues[78]. Future research should employ longitudinal data to more accurately elucidate causal relationships between variables. Second, a substantial number of samples (n = 11,270) were excluded due to missing covariate data, which may introduce selection bias. Future studies should consider employing multiple imputation techniques to handle missing data and conduct sensitivity analyses to assess the robustness of the findings. Additionally, the classification of occupational types was simplistic, dividing them merely into "agricultural" and "non-agricultural," failing to capture the heterogeneity within agricultural work, such as subsistence versus commercial farming and land ownership differences. Future research should refine occupational classifications to more comprehensively understand the impact of occupational types on depressive symptoms. The study also employed logistic regression to analyze depressive symptoms, a common outcome variable, which may overestimate odds ratios. Future studies might consider alternative regression models, such as Poisson regression, to obtain more accurate estimates. Although this study found that life satisfaction had a mediating effect between agricultural work and depressive symptoms, the effect was weak, accounting for only 5.13% of the total effect. This may be due to the omission of other potential mediating variables, such as economic stability and pesticide exposure. Future research should explore additional mediators to more fully understand the mechanisms by which agricultural work influences depressive symptoms. Finally, the 2015 data used in this study may not capture recent socioeconomic changes in rural China, such as digital development and policy shifts. Additionally, mental health research involves sensitive information, necessitating careful ethical considerations to ensure participant privacy and informed consent during data collection and analysis. Future studies should focus on the temporal relevance of data and enhance ethical review and participant protection measures.”

Best regards,

Yuan Yao Chen

Attachment

Submitted filename: Reply to reviewer3.docx

pone.0325912.s005.docx (16.3KB, docx)

Decision Letter 4

Zahra Lorigooini

PONE-D-24-33298R4Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediatorPLOS ONE

Dear Dr. Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

  • To ensure clarity and thoroughness in addressing their comments, we kindly request that you respond to each reviewer’s comment separately in your response file, rather than addressing all comments collectively.

Please submit your revised manuscript by Jun 16 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Zahra Lorigooini

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/ . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org . Please note that Supporting Information files do not need this step.

PLoS One. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912.r010

Author response to Decision Letter 5


7 May 2025

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Dear Editor,

Thank you for your kind reminder, we have searched and checked all the cited references, and made sure that all the list is complete, correct and no paper were retracted in present. Thank you for the kind reminder again!

Best Regards,

Yuan Yao Chen

Attachment

Submitted filename: response.docx

pone.0325912.s006.docx (10.7KB, docx)

Decision Letter 5

Zahra Lorigooini

PONE-D-24-33298R5Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediatorPLOS ONE

Dear Dr. Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================

ACADEMIC EDITOR:

I have carefully reviewed the manuscript and would like to share some editorial suggestions to enhance the clarity and professionalism of the text:

  • Inconsistent Spacing:

    • In several instances, there are missing spaces after periods and commas, e.g., "depressive symptoms.Life satisfaction" should be "depressive symptoms. Life satisfaction."
    • Extra spaces were found between words in sentences like "working in agriculture," which should be corrected to "working in agriculture."
    • In many instances, reference numbers are directly attached to the last word, e.g., "study shows that depression is prevalent among older adults[12]" should be "study shows that depression is prevalent among older adults [12]."
  • Improper Hyphenation and Spacing:

    Some compound terms such as "self-employedagricultural workers" lack appropriate spacing. This should be revised to "self-employed agricultural workers."

  • Extra Periods:

    • In certain parts of the manuscript, extra periods were identified, e.g., "data were analyzed.." This should be corrected to "data were analyzed."
  • Spacing Around Statistical Values:

    • In statistical reporting, there should be a space before and after inequality symbols, e.g., "p<0.001" should be "p < 0.001."
  • Inconsistent Use of Punctuation:

    • Some sections contain inconsistent use of commas and semicolons. Please ensure that list items are separated correctly and that sentences are punctuated properly.
  • Suggestions for Improvement:

    • I recommend a thorough proofreading to ensure consistency in spacing, punctuation, and formatting.
    • Utilizing tools such as Grammarly or the built-in proofing tools in Microsoft Word may assist in identifying minor errors.

Note: I have previously mentioned that the manuscript should be edited according to the PLOS ONE formatting guidelines. However, the issues mentioned above are still present in the text. I recommend careful alignment with the PLOS ONE standards to avoid further revisions.

I hope these suggestions help in enhancing the quality and readability of the manuscript. If you require further clarification or assistance with revisions, please feel free to reach out.

==============================

Please submit your revised manuscript by Jun 28 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Zahra Lorigooini

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/ . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org . Please note that Supporting Information files do not need this step.

PLoS One. 2025 Aug 13;20(8):e0325912. doi: 10.1371/journal.pone.0325912.r012

Author response to Decision Letter 6


19 May 2025

Dear Editor,

We sincerely appreciate the editor’s meticulous review and constructive feedback. Below is our point-by-point responsethe issues raised, along with the revisions made to the manuscript:

I have carefully reviewed the manuscript and would like to share some editorial suggestions to enhance the clarity and professionalism of the text:

Inconsistent Spacing:

In several instances, there are missing spaces after periods and commas, e.g., "depressive symptoms.Life satisfaction" should be "depressive symptoms. Life satisfaction."

Authors’ reply�Thank you for your thoroughly check and review, all instances of missing or extra spacing have been corrected. For example, revised“depressive symptoms.Life satisfaction”to“depressive symptoms. Life satisfaction.” Adjusted spacing in phrases like “working in agriculture,” to ensure proper formatting, which were all highlighted in yellow.

Extra spaces were found between words in sentences like "working in agriculture," which should be corrected to "working in agriculture."

In many instances, reference numbers are directly attached to the last word, e.g., "study shows that depression is prevalent among older adults[12]" should be "study shows that depression is prevalent among older adults [12]."

Authors’ reply�Thank you for your thoroughly check and review, spaces have been added between text and reference numbers in all instances. We have made sure all reference numbers with spaces attached to the last word.

Improper Hyphenation and Spacing:

Some compound terms such as "self-employedagricultural workers" lack appropriate spacing. This should be revised to "self-employed agricultural workers."

Authors’ reply�Thank you for your thoroughly check and review, all compound terms have been standardized with appropriate spacing: Corrected “self-employedagricultural workers” to “self-employed agricultural workers.”The corrections were in yellow in the revised manuscript.

Extra Periods:

In certain parts of the manuscript, extra periods were identified, e.g., "data were analyzed.." This should be corrected to "data were analyzed."

Spacing Around Statistical Values:

In statistical reporting, there should be a space before and after inequality symbols, e.g., "p<0.001" should be "p < 0.001."

Authors’ reply�Thank you for your thoroughly check and review, redundant periods have been removed. Examples include: Revised “data were analyzed..” to “data were analyzed.”Checked for duplicated punctuation in tables, footnotes, and body text.

Inconsistent Use of Punctuation:

Some sections contain inconsistent use of commas and semicolons. Please ensure that list items are separated correctly and that sentences are punctuated properly.

Suggestions for Improvement:

I recommend a thorough proofreading to ensure consistency in spacing, punctuation, and formatting.

Utilizing tools such as Grammarly or the built-in proofing tools in Microsoft Word may assist in identifying minor errors.

Authors’ reply�Thank you for your thoroughly check and review, punctuation has been standardized: Corrected list separators (e.g., revised “factors such as work pressure [5], work hours [6, 7]” to ensure commas and semicolons align with journal guidelines). Adjusted sentence structures for clarity and consistency (e.g., removed misplaced semicolons in tables). Moreover, we’ve check our grammar and revised some sentences details.

Best regards,

Yuan Yao Chen

Attachment

Submitted filename: Reply 5.19.docx

pone.0325912.s007.docx (12.1KB, docx)

Decision Letter 6

Zahra Lorigooini

<p>Can working in agriculture have a favorable effect on depressive symptoms?Life satisfaction as a mediator

PONE-D-24-33298R6

Dear Dr. Chen,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager®  and clicking the ‘Update My Information' link at the top of the page. If you have any questions relating to publication charges, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Zahra Lorigooini

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Acceptance letter

Zahra Lorigooini

PONE-D-24-33298R6

PLOS ONE

Dear Dr. Chen,

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now being handed over to our production team.

At this stage, our production department will prepare your paper for publication. This includes ensuring the following:

* All references, tables, and figures are properly cited

* All relevant supporting information is included in the manuscript submission,

* There are no issues that prevent the paper from being properly typeset

You will receive further instructions from the production team, including instructions on how to review your proof when it is ready. Please keep in mind that we are working through a large volume of accepted articles, so please give us a few days to review your paper and let you know the next and final steps.

Lastly, if your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

If we can help with anything else, please email us at customercare@plos.org.

Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Prof. Zahra Lorigooini

Academic Editor

PLOS ONE

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    Attachment

    Submitted filename: Reply to editor, reviewer1, reviewer2 and reviewer3.docx

    pone.0325912.s002.docx (46.5KB, docx)
    Attachment

    Submitted filename: revised manuscript 3.0.docx

    pone.0325912.s003.docx (146.3KB, docx)
    Attachment

    Submitted filename: renamed_36f54.docx

    pone.0325912.s004.docx (11.9KB, docx)
    Attachment

    Submitted filename: Reply to reviewer3.docx

    pone.0325912.s005.docx (16.3KB, docx)
    Attachment

    Submitted filename: response.docx

    pone.0325912.s006.docx (10.7KB, docx)
    Attachment

    Submitted filename: Reply 5.19.docx

    pone.0325912.s007.docx (12.1KB, docx)

    Data Availability Statement

    All relevant data can be found at the following website: http://CHARLS.pku.edu.cn/en. We conducted the study just through downloading the three questionaires called "Demographic_Background," "Health_Status_and_Functioning" and "Work_Retirement_and_Pension" from CHARLS 2015, all of which can be found at: https://charls.charlsdata.com/pages/data/111/en.html.


    Articles from PLOS One are provided here courtesy of PLOS

    RESOURCES