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. 1983 Oct;47(4):440–444.

Correlation of surface and internal ultrastructural changes in cells infected with foot-and-mouth disease virus.

J Polatnick, S H Wool
PMCID: PMC1235972  PMID: 6321000

Abstract

The surfaces of primary and continuous line cell cultures displayed the same sequence of morphological changes during the course of infection with foot-and-mouth disease virus. These changes could be classified into four broad stages: I) cells were flattened, closely attached to one another and microvilli appeared, II) cells rounded, microvilli began to disappear and the cells started to separate from one another by cytoplasmic strands, III) cells were discrete, rounded structures and IV) cells were rounded and had numerous attached buds, some of which contained virus. The internal changes included the appearance of increasing amounts of smooth membranous vacuoles lined with the viral induced RNA polymerase and the presence of buds, some with viral particles inside. While the different cell cultures showed similar internal and external changes as a result of infection, they responded to infection at different rates and contained subpopulations of resistant cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACHRACH H. L., HESS W. R., CALLIS J. J. Foot-and-mouth disease virus: its growth and cytopathogenicity in tissue culture. Science. 1955 Dec 30;122(3183):1269–1270. doi: 10.1126/science.122.3183.1269. [DOI] [PubMed] [Google Scholar]
  2. Bienz K., Egger D., Rasser Y., Bossart W. Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology. Virology. 1980 Jan 30;100(2):390–399. doi: 10.1016/0042-6822(80)90530-9. [DOI] [PubMed] [Google Scholar]
  3. Breese S. S., Jr, Graves J. H. Electron microscopic observation of crystalline arrays of foot-and-mouth disease virus. J Bacteriol. 1966 Dec;92(6):1835–1837. doi: 10.1128/jb.92.6.1835-1837.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DALES S., EGGERS H. J., TAMM I., PALADE G. E. ELECTRON MICROSCOPIC STUDY OF THE FORMATION OF POLIOVIRUS. Virology. 1965 Jul;26:379–389. doi: 10.1016/0042-6822(65)90001-2. [DOI] [PubMed] [Google Scholar]
  5. Dinka S. K., Swaney L. M., Vicar J. W. Selection of a stable clone of the MVPK-1 fetal porcine kidney cell for assays of foot-and-month disease virus. Can J Microbiol. 1977 Mar;23(3):295–299. doi: 10.1139/m77-043. [DOI] [PubMed] [Google Scholar]
  6. House J. A., Yedloutschnig R. J. Sensitivity of seven different types of cell cultures to three serotypes of foot-and-mouth disease virus. Can J Comp Med. 1982 Apr;46(2):186–189. [PMC free article] [PubMed] [Google Scholar]
  7. KHERA K. S., DHILLON S. S. Morphology of goat kidney monolayer cultures and cellular changes produced by foot-and-mouth disease virus. Am J Vet Res. 1962 Nov;23:1294–1299. [PubMed] [Google Scholar]
  8. MELENDEZ L., GAGGERO A., RODRIGUEZ R., NORAMBUENA M. Multiplication of foot and mouth disease virus in adult kidney, and embryonic lung and heart bovine tissue cultures. Proc Soc Exp Biol Med. 1957 Aug-Sep;95(4):696–697. doi: 10.3181/00379727-95-23333. [DOI] [PubMed] [Google Scholar]
  9. POLATNICK J., BACHRACH H. L. PRODUCTION AND PURIFICATION OF MILLIGRAM AMOUNTS OF FOOT-AND-MOUTH DISEASE VIRUS FROM BABY HAMSTER KIDNEY CELL CULTURES. Appl Microbiol. 1964 Jul;12:368–373. doi: 10.1128/am.12.4.368-373.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Polatnick J. Isolation of a foot-and-mouth disease polyuridylic acid polymerase and its inhibition by antibody. J Virol. 1980 Feb;33(2):774–779. doi: 10.1128/jvi.33.2.774-779.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Polatnick J., Wool S. H. Localization of foot-and-mouth disease--RNA synthesis on newly formed cellular smooth membranous vacuoles. Arch Virol. 1982;71(3):207–215. doi: 10.1007/BF01314872. [DOI] [PubMed] [Google Scholar]
  12. Polatnick J., Wool S. Characterization of a 70S polyuridylic acid polymerase isolated from foot-and-mouth disease virus-infected cells. J Virol. 1981 Dec;40(3):881–889. doi: 10.1128/jvi.40.3.881-889.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SELLERS R. F., BURT L. M., CUMMING A., STEWART D. L. The behaviour of strains of the virus of foot-and-mouth disease in pig,calf,ox and lamb kidney tissue cultures. Arch Gesamte Virusforsch. 1960;9:637–646. doi: 10.1007/BF01242149. [DOI] [PubMed] [Google Scholar]
  14. Snowdon W. A. Growth of foot-and mouth disease virus in monolayer cultures of calf thyroid cells. Nature. 1966 Jun 4;210(5040):1079–1080. doi: 10.1038/2101079a0. [DOI] [PubMed] [Google Scholar]
  15. Wool S. H., Polatnick J., Knudsen R. C. Ultrastructural changes and antigen localization in tissues from foot-and-mouth disease virus-infected guinea-pigs. Vet Microbiol. 1982 Nov;7(5):391–400. doi: 10.1016/0378-1135(82)90055-4. [DOI] [PubMed] [Google Scholar]
  16. Yilma T., McVicar J. W., Breese S. S. Pre-lytic release of foot-and-mouth disease virus in cytoplasmic blebs. J Gen Virol. 1978 Oct;41(1):105–114. doi: 10.1099/0022-1317-41-1-105. [DOI] [PubMed] [Google Scholar]

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