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Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1984 Oct;48(4):431–433.

Comparison of selenium levels and glutathione peroxidase activity in bovine whole blood.

L D Koller, P J South, J H Exon, G A Whitbeck, J Maas
PMCID: PMC1236100  PMID: 6509373

Abstract

Blood glutathione peroxidase activity and selenium levels were found to correlate well, indicating that glutathione peroxidase activity can be used to assess blood selenium levels in beef cattle. The glutathione peroxidase activity of blood is less stable than is the selenium concentration but when blood was stored at 4 degrees C, the glutathione peroxidase activity remained constant for seven days.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen W. M., Parr W. H., Anderson P. H., Berett S., Bradley R. Selenium and the activity of glutathione peroxidase in bovien erythrocytes. Vet Rec. 1975 Apr 19;96(16):360–361. doi: 10.1136/vr.96.16.360. [DOI] [PubMed] [Google Scholar]
  2. Anderson P. H., Berrett S., Patterson D. S. The biological selenium status of livestock in Britain as indicated by sheep erythrocyte glutathione peroxidase activity. Vet Rec. 1979 Mar 17;104(11):235–238. doi: 10.1136/vr.104.11.235. [DOI] [PubMed] [Google Scholar]
  3. Backall K. A., Scholz R. W. Reference values for a field test to estimate inadequate glutathione peroxidase activity and selenium status in the blood of cattle. Am J Vet Res. 1979 May;40(5):733–738. [PubMed] [Google Scholar]
  4. Berrett S., Hebert C. N. A semi-quantitative spot test for glutathione peroxidase in blood of cattle and sheep for the assessemt of biological selenium status. Vet Rec. 1979 Aug 18;105(7):145–146. doi: 10.1136/vr.105.7.145. [DOI] [PubMed] [Google Scholar]
  5. Board P. G., Peter D. W. A simple test for glutathione peroxidase and selenium deficiency. Vet Rec. 1976 Aug 21;99(8):144–145. doi: 10.1136/vr.99.8.144. [DOI] [PubMed] [Google Scholar]
  6. COUSINS F. B. A fluorimetric microdetermination of selenium in biological material. Aust J Exp Biol Med Sci. 1960 Feb;38:11–15. doi: 10.1038/icb.1960.2. [DOI] [PubMed] [Google Scholar]
  7. Flohe L., Günzler W. A., Schock H. H. Glutathione peroxidase: a selenoenzyme. FEBS Lett. 1973 May 15;32(1):132–134. doi: 10.1016/0014-5793(73)80755-0. [DOI] [PubMed] [Google Scholar]
  8. Hoffman C., Rivinus B., Swanson L. Effect of intramuscular administration of selenium and vitamine E in dairy heifers on erythrocyte glutathione peroxidase activity and blood selenium levels. J Anim Sci. 1978 Jul;47(1):192–197. doi: 10.2527/jas1978.471192x. [DOI] [PubMed] [Google Scholar]
  9. Koller L. D., South P. J., Exon J. H., Whitbeck G. A. Selenium deficiency of beef cattle in Idaho and Washington and a practical means of prevention. Cornell Vet. 1983 Oct;73(4):323–332. [PubMed] [Google Scholar]
  10. McMurray C. H., Blanchflower W. J. The levels of selenium and glutathione peroxidase activity in blood of sheep, cows and pigs. Res Vet Sci. 1976 Mar;20(2):229–231. [PubMed] [Google Scholar]
  11. Oh S. H., Pope A. L., Hoekstra W. G. Dietary selenium requirements of sheep fed a practical-type diet as assessed by tissue glutathione peroxidase and other criteria. J Anim Sci. 1976 Apr;42(4):984–992. doi: 10.2527/jas1976.424984x. [DOI] [PubMed] [Google Scholar]
  12. Paglia D. E., Valentine W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967 Jul;70(1):158–169. [PubMed] [Google Scholar]
  13. Rotruck J. T., Pope A. L., Ganther H. E., Swanson A. B., Hafeman D. G., Hoekstra W. G. Selenium: biochemical role as a component of glutathione peroxidase. Science. 1973 Feb 9;179(4073):588–590. doi: 10.1126/science.179.4073.588. [DOI] [PubMed] [Google Scholar]
  14. Scholz R. W., Cook L. S., Todhunter D. A. Distribution of selenium-dependent and nonselenium-dependent glutathione peroxidase activity in tissues of young cattle. Am J Vet Res. 1981 Oct;42(10):1724–1729. [PubMed] [Google Scholar]
  15. Scholz R. W., Hutchinson L. J. Distribution of glutathione peroxidase activity and selenium in the blood of dairy cows. Am J Vet Res. 1979 Feb;40(2):245–249. [PubMed] [Google Scholar]
  16. Scholz R. W., Todhunter D. A., Cook L. S. Selenium content and glutathione peroxidase activity in tissues of young cattle fed supplemented whole milk diets. Am J Vet Res. 1981 Oct;42(10):1718–1723. [PubMed] [Google Scholar]
  17. Sheppard A. D., Millar K. R. Stability of glutathione peroxidase in ovine blood samples under various storage conditions and the response of this enzyme to different methods of selenium supplementation. N Z Vet J. 1981 May;29(5):77–80. doi: 10.1080/00480169.1981.34805. [DOI] [PubMed] [Google Scholar]
  18. Thompson K. G., Fraser A. J., Harrop B. M., Kirk J. A., Bullians J., Cordes D. O. Glutathione peroxidase activity and selenium concentration in bovine blood and liver as indicators of dietary selenium intake. N Z Vet J. 1981 Jan-Feb;29(1-2):3–6. doi: 10.1080/00480169.1981.34776. [DOI] [PubMed] [Google Scholar]
  19. Whanger P. D., Weswig P. H., Schmitz J. A., Oldfield J. E. Effects of selenium and vitamin E on blood selenium levels, tissue glutathione peroxidase activities and white muscle disease in sheep fed purified or hay diets. J Nutr. 1977 Jul;107(7):1298–1307. doi: 10.1093/jn/107.7.1298. [DOI] [PubMed] [Google Scholar]
  20. Wilson P. S., Judson G. J. Glutathione peroxidase activity in bovine and ovine erythrocytes in relation to blood selenium concentration. Br Vet J. 1976 Jul-Aug;132(4):428–434. doi: 10.1016/s0007-1935(17)34644-4. [DOI] [PubMed] [Google Scholar]

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