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. 1985 Jan;49(1):79–87.

Enterotoxin plasmids in bovine and porcine enterotoxigenic Escherichia coli of O groups 9, 20, 64 and 101.

N M Harnett, C L Gyles
PMCID: PMC1236122  PMID: 3886109

Abstract

Fourteen strains of Escherichia coli of serogroups characteristic of porcine class 2 enterotoxigenic E. coli isolated from pigs or calves were selected for genetic studies. The strains were examined for their ability to cotransfer a number of plasmid-mediated properties during conjugation with E. coli K-12. These properties were antibiotic resistance, and the production of heat-stable enterotoxin, the K99 antigen and colicin and the ability to ferment raffinose. Distinction was made between the two types of heat-stable enterotoxin, STa and STb. All 14 strains were antibiotic resistant and 11 of them cotransferred antibiotic resistance and heat-stable enterotoxin. One strain which transferred heat-stable enterotoxin also transferred the raffinose gene. Among six K99-positive strains which transferred heat-stable enterotoxin, five always cotransferred K99. Three strains had 100% cotransfer of colicin as well as heat-stable enterotoxin and K99. Drug resistance determinants were cotransferred at high frequency with heat-stable enterotoxin for six of eight multiple drug resistant enterotoxigenic E. coli. A 100% cotransfer of combinations of heat-stable enterotoxin, K99, colicin and antibiotic resistance was often associated with a single plasmid band on agarose gel electrophoresis. For some strains, the genes for STa and STb were on the same plasmid and for others they were on separate plasmids. The enterotoxin plasmids ranged in size from 5.2 to 85 Mdal. Heterogeneity in molecular size occurred among enterotoxin plasmids in E. coli of the same serogroup and recovered from the same animal host species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Natkin E. Transduction of resistance determinants and R factors of the transfer systems by phage Plkc. Mol Gen Genet. 1972;114(3):261–265. doi: 10.1007/BF01788895. [DOI] [PubMed] [Google Scholar]
  2. Crisona N. J., Nowak J. A., Nagaishi H., Clark A. J. Transposon-mediated conjugational transmission of nonconjugative plasmids. J Bacteriol. 1980 May;142(2):701–713. doi: 10.1128/jb.142.2.701-713.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FREDERICQ P. Colicins. Annu Rev Microbiol. 1957;11:7–22. doi: 10.1146/annurev.mi.11.100157.000255. [DOI] [PubMed] [Google Scholar]
  4. Franklin A., Söderlind, Möllby R. Plasmids coding for enterotoxins, K88 antigen and colicins in porcine Escherichia coli strains of O-group 149. Med Microbiol Immunol. 1981;170(2):63–72. doi: 10.1007/BF02122670. [DOI] [PubMed] [Google Scholar]
  5. Guinée P. A., Jansen W. H., Agterberg C. M. Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity. Infect Immun. 1976 May;13(5):1369–1377. doi: 10.1128/iai.13.5.1369-1377.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gyles C. L., Barnum D. A. Escherichia coli in ligated segments of pig intestine. J Pathol Bacteriol. 1967 Jul;94(1):189–194. doi: 10.1002/path.1700940124. [DOI] [PubMed] [Google Scholar]
  7. Gyles C. L. Limitations of the infant mouse test for Escherichia coli heat stable enterotoxin. Can J Comp Med. 1979 Oct;43(4):371–379. [PMC free article] [PubMed] [Google Scholar]
  8. Gyles C. L., Palchaudhuri S., Maas W. K. Naturally occurring plasmid carrying genes for enterotoxin production and drug resistance. Science. 1977 Oct 14;198(4313):198–199. doi: 10.1126/science.333581. [DOI] [PubMed] [Google Scholar]
  9. Gyles C. L., Stevens J. B., Craven J. A. A study of Escherichia coli strains isolated from pigs with gastro-intestinal disease. Can J Comp Med. 1971 Jul;35(3):258–266. [PMC free article] [PubMed] [Google Scholar]
  10. Gyles C., So M., Falkow S. The enterotoxin plasmids of Escherichia coli. J Infect Dis. 1974 Jul;130(1):40–49. doi: 10.1093/infdis/130.1.40. [DOI] [PubMed] [Google Scholar]
  11. Harnett N. M., Gyles C. L. Enterotoxigenicity of bovine and porcine Escherichia coli of O groups 8, 9, 20, 64, 101, and X46. Am J Vet Res. 1983 Jul;44(7):1210–1214. [PubMed] [Google Scholar]
  12. Helmuth R., Stephan R., Bulling E., van Leeuwen W. J., van Embden J. D., Guinée P. A., Portnoy D., Falkow S. R-factor cointegrate formation in Salmonella typhimurium bacteriophage type 201 strains. J Bacteriol. 1981 May;146(2):444–452. doi: 10.1128/jb.146.2.444-452.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hooykaas P. J., den Dulk-Ras H., Schilperoort R. A. Molecular mechanism of Ti plasmid mobilization by R plasmids: isolation of Ti plasmids with transposon-insertions in Agrobacterium tumefaciens. Plasmid. 1980 Jul;4(1):64–75. doi: 10.1016/0147-619x(80)90083-9. [DOI] [PubMed] [Google Scholar]
  14. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Milliken C. E., Clowes R. C. Molecular structure of an R factor, its component drug-resistance determinants and transfer factor. J Bacteriol. 1973 Feb;113(2):1026–1033. doi: 10.1128/jb.113.2.1026-1033.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moon H. W., Kohler E. M., Schneider R. A., Whipp S. C. Prevalence of pilus antigens, enterotoxin types, and enteropathogenicity among K88-negative enterotoxigenic Escherichia coli from neonatal pigs. Infect Immun. 1980 Jan;27(1):222–230. doi: 10.1128/iai.27.1.222-230.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moon H. W., Whipp S. C. Development of resistance with age by swine intestine to effects of enteropathogenic Escherichia coli. J Infect Dis. 1970 Sep;122(3):220–223. doi: 10.1093/infdis/122.3.220. [DOI] [PubMed] [Google Scholar]
  18. Mullan N. A., Burgess M. N., Newsome P. M. Characterization of a partially purified methanol-soluble heat-stable Escherichia coli enterotoxin in infant mice. Infect Immun. 1978 Mar;19(3):779–784. doi: 10.1128/iai.19.3.779-784.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagy B., Moon H. W., Isaacson R. E. Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infect Immun. 1977 Apr;16(1):344–352. doi: 10.1128/iai.16.1.344-352.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ohtsubo E., Zenilman M., Ohtsubo H. Plasmids containing insertion elements are potential transposons. Proc Natl Acad Sci U S A. 1980 Feb;77(2):750–754. doi: 10.1073/pnas.77.2.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reis M. H., Heloiza M., Affonso T., Trabulsi L. R., Mazaitis A. J., Maas R., Maas W. K. Transfer of a CFA/I-ST plasmid promoted by a conjugative plasmid in a strain of Escherichia coli of serotype O128ac:H12. Infect Immun. 1980 Jul;29(1):140–143. doi: 10.1128/iai.29.1.140-143.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scotland S. M., Gross R. J., Cheasty T., Rowe B. The occurrence of plasmids carrying genes for both enterotoxin production and drug resistance in Escherichia coli of human origin. J Hyg (Lond) 1979 Dec;83(3):531–538. doi: 10.1017/s0022172400026383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shapiro J. A. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1933–1937. doi: 10.1073/pnas.76.4.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sivaswamy G., Gyles C. L. The prevalence of enterotoxigenic Escherichia coli in the feces of calves with diarrhea. Can J Comp Med. 1976 Jul;40(3):241–246. [PMC free article] [PubMed] [Google Scholar]
  25. Skerman F. J., Formal S. B., Falkow S. Plasmid-associated enterotoxin production in a strain of Escherichia coli isolated from humans. Infect Immun. 1972 Apr;5(4):622–624. doi: 10.1128/iai.5.4.622-624.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith H. W., Gyles C. L. The relationship between different transmissible plasmids introduced by F into the same strain of Escherichia coli K12. J Gen Microbiol. 1970 Aug;62(3):227–285. doi: 10.1099/00221287-62-3-277. [DOI] [PubMed] [Google Scholar]
  27. Smith H. W., Linggood M. A. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. J Med Microbiol. 1972 May;5(2):243–250. doi: 10.1099/00222615-5-2-243. [DOI] [PubMed] [Google Scholar]
  28. So M., Boyer H. W., Betlach M., Falkow S. Molecular cloning of an Escherichia coli plasmid determinant than encodes for the production of heat-stable enterotoxin. J Bacteriol. 1976 Oct;128(1):463–472. doi: 10.1128/jb.128.1.463-472.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. So M., Heffron F., Falkow S. Method for the genetic labeling of cryptic plasmids. J Bacteriol. 1978 Mar;133(3):1520–1523. doi: 10.1128/jb.133.3.1520-1523.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. So M., Heffron F., McCarthy B. J. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 1979 Feb 8;277(5696):453–456. doi: 10.1038/277453a0. [DOI] [PubMed] [Google Scholar]
  31. So M., McCarthy B. J. Nucleotide sequence of the bacterial transposon Tn1681 encoding a heat-stable (ST) toxin and its identification in enterotoxigenic Escherichia coli strains. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4011–4015. doi: 10.1073/pnas.77.7.4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stieglitz H., Fonseca R., Olarte J., Kupersztoch-Portnoy Y. M. Linkage of heat-stable enterotoxin activity and ampicillin resistance in a plasmid isolated from an Escherichia coli strain of human origin. Infect Immun. 1980 Nov;30(2):617–620. doi: 10.1128/iai.30.2.617-620.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Williams P. H., Sedgwick M. I., Evans N., Turner P. J., George R. H., McNeish A. S. Adherence of an enteropathogenic strain of Escherichia coli to human intestinal mucosa is mediated by a colicinogenic conjugative plasmid. Infect Immun. 1978 Nov;22(2):393–402. doi: 10.1128/iai.22.2.393-402.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Willshaw G. A., Smith H. R., McConnell M. M., Barclay E. A., Krnjulac J., Rowe B. Genetic and molecular studies of plasmids coding for colonization factor antigen I and heat-stable enterotoxin in several escherichia coli serotypes. Infect Immun. 1982 Sep;37(3):858–868. doi: 10.1128/iai.37.3.858-868.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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