Skip to main content
NCBI home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1985 Jan;49(1):91–94.

Adjuvant enhancement of humoral immune response to chemically inactivated bovine viral diarrhea virus.

K S Chen, D W Johnson, C C Muscoplat
PMCID: PMC1236124  PMID: 2985217

Abstract

Potentiation of the antibody response to inactivated bovine viral diarrhea virus by immunological adjuvants was studied in guinea pigs and cattle. The inactivated bovine viral diarrhea virus alone was demonstrated to be a weak immunogen. Addition of either 2 mg per mL diethylaminoethyl-dextran or 5% alhydrogel to inactivated bovine viral diarrhea virus did not or only slightly stimulated the antibody response; the combined adjuvants induced a significantly higher titer. A higher concentration (20 mg per mL) of diethylaminoethyl-dextran, on the contrary, suppressed the immune potentiation by the dual adjuvants. Combination of Bordetella bronchiseptica and alhydrogel adjuvants stimulated a high titer of antibody. The titer was further elevated upon revaccination and was significantly higher than that induced by alhydrogel alone. In cattle, alhydrogel enhanced the immune response and the additional inclusion of diethylaminoethyl-dextran did not cause a significant potentiation of the immunity. However, the antibody decay rate was significantly slower when stimulated by the combined adjuvants.

Full text

PDF
91

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BINN L. N., HILLEMAN M. R. A guinea pig potency test for adenovirus vaccine. J Immunol. 1960 Jan;84:20–26. [PubMed] [Google Scholar]
  2. Bogaerts W. J., Durville-van der Oord Immunization of mice against encephalomyocarditis virus. II. Intraperitoneal and respiratory immunization with ultraviolet-inactivated vaccine: effect of Bordetella pertussis extract on the immune response. Infect Immun. 1972 Oct;6(4):513–517. doi: 10.1128/iai.6.4.513-517.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FLEMING D. S., GREENBERG L., BEITH E. M. The use of combined antigens in the immunization of infants. Can Med Assoc J. 1948 Aug;59(2):101–105. [PMC free article] [PubMed] [Google Scholar]
  4. Fernelius A. L., Classick L. G., Smith R. L. Evaluation of -propiolactone-inactivated- and chloroform-treated-virus vaccines against bovine viral diarrhea-mucosal disease. Am J Vet Res. 1972 Jul;33(7):1421–1431. [PubMed] [Google Scholar]
  5. Kolar J. R., Jr, Shechmeister I. L., Strack L. E. Field experiments with formalin-killed-virus vaccine against infectious bovine rhinotracheitis, bovine viral diarrhea and parainfluenza-3. Am J Vet Res. 1973 Nov;34(11):1469–1471. [PubMed] [Google Scholar]
  6. Lehrer S. B., Tan E. M., Vaughan J. H. Extraction and partial purification of the histamine-sensitizing factor of Bordetella pertussis. J Immunol. 1974 Jul;113(1):18–26. [PubMed] [Google Scholar]
  7. McClurkin A. W., Coria M. F., Smith R. L. Evaluation of acetylethyleneimine-killed bovine viral diarrhea-mucosal disease virus (BVD) vaccine for prevention of BVD infection of the fetus. Proc Annu Meet U S Anim Health Assoc. 1975;(79):114–123. [PubMed] [Google Scholar]
  8. McClurkin A. W., Pirtle E. C., Coria M. F., Smith R. L. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch Gesamte Virusforsch. 1974;45(3):285–289. doi: 10.1007/BF01249692. [DOI] [PubMed] [Google Scholar]
  9. Murgo A. J., Athanassiades T. J. Adjuvant effect of Bordetella pertussis vaccine to sheep erythrocytes: enhancement of antibody formation by using subcutaneous administration of adjuvant and antigen. Infect Immun. 1975 Nov;12(5):969–977. doi: 10.1128/iai.12.5.969-977.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pieroni R. E., Levine L. Adjuvant principle of pertussis vaccine in the mouse. Nature. 1966 Sep 24;211(5056):1419–1420. doi: 10.1038/2111419a0. [DOI] [PubMed] [Google Scholar]
  11. Rossi C. R., Kiesel G. K. Microtiter tests for detecting antibody in bovine serum to parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and bovine virus diarrhea virus. Appl Microbiol. 1971 Jul;22(1):32–36. doi: 10.1128/am.22.1.32-36.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wittmann G., Dietzschold B., Bauer K. Some investigations on the adjuvant mechanism of DEAE dextran. Arch Virol. 1975;47(3):225–235. doi: 10.1007/BF01317810. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES