Abstract
A comparison of titers was made between an egg assay and a direct fluorescent antibody assay of three chlamydial strains propagated in Vero cells with and without cortisone plus cytochalasin B. The titer of NJ-1 strain was similar in the egg titration and the fluorescent antibody assay in the untreated sample and a little lower for the sample treated with cytochalasin B and cortisone. The SCT and CDC strains had approximately the same titers in the egg titration and the fluorescent antibody assay for samples with and without the antimetabolites.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FURNESS G., GRAHAM D. M., REEVE P. The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. J Gen Microbiol. 1960 Dec;23:613–619. doi: 10.1099/00221287-23-3-613. [DOI] [PubMed] [Google Scholar]
- Griffiths M. S., Ainsworth S., Pearce J. H. Infectivity titration of guinea-pig inclusion conjunctivitis agent in irradiated McCoy cells. J Gen Microbiol. 1976 Aug;96(2):249–256. doi: 10.1099/00221287-95-2-249. [DOI] [PubMed] [Google Scholar]
- HAHON N., NAKAMURA R. M. QUANTITATIVE ASSAY OF PSITTACOSIS VIRUS BY THE FLUORESCENT CELL-COUNTING TECHNIQUE. Virology. 1964 Jun;23:203–208. doi: 10.1016/0042-6822(64)90283-1. [DOI] [PubMed] [Google Scholar]
- Page L. A., Derieux W. T., Cutlip R. C. An epornitic of fatal chlamydiosis (ornithosis) in South Carolina turkeys. J Am Vet Med Assoc. 1975 Jan 15;166(2):175–178. [PubMed] [Google Scholar]
- Spears P., Storz J. Biotyping of Chlamydia psittaci based on inclusion morphology and response to diethylaminoethyl-dextran and cycloheximide. Infect Immun. 1979 Apr;24(1):224–232. doi: 10.1128/iai.24.1.224-232.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spears P., Storz J. Chlamydia psittaci: growth characteristics and enumeration of serotypes 1 and 2 in cultured cells. J Infect Dis. 1979 Dec;140(6):959–967. doi: 10.1093/infdis/140.6.959. [DOI] [PubMed] [Google Scholar]
- Tessler J. Effects of cortisone, cytochalasin B and cycloheximide on strains of Chlamydia psittaci in cell cultures. Can J Comp Med. 1983 Jul;47(3):352–357. [PMC free article] [PubMed] [Google Scholar]
- Tessler J., Hess W. R., Pan I. C., Trautman R. Immunofluorescence plaque assay for African swine fever virus. Can J Comp Med. 1974 Oct;38(4):443–447. [PMC free article] [PubMed] [Google Scholar]
- WEISS E., HUANG J. S. The infected cell count method of titration of feline pneumonitis virus. J Infect Dis. 1954 Mar-Apr;94(2):107–125. doi: 10.1093/infdis/94.2.107. [DOI] [PubMed] [Google Scholar]