Abstract
Blood and bone marrow morphology were studied sequentially in dogs during experimental inflammation induced by intramuscular injection of turpentine. Depletion of the bone marrow storage pool of mature neutrophils and an increase in mitotic activity and number of early granulocyte precursors were evident within 24 hours. During the next three days, intense granulocytic hyperplasia resulted in replenishment of the bone marrow storage pool. Neutrophils with foamy vacuolation and increased basophilia of the cytoplasm (toxic neutrophils) were present in the blood by eight hours postinjection. The number of toxic neutrophils paralleled the intensity of clinical signs and changes in rectal temperature but not the number of band neutrophils. This indicates that changes in number of toxic neutrophils in sequential leukograms can be a prognostic indicator in dogs with severe inflammation.
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- Gossett K. A., Carakostas M. C. Effect of EDTA on morphology of neutrophils of healthy dogs and dogs with inflammation. Vet Clin Pathol. 1984;13(2):22–25. doi: 10.1111/j.1939-165x.1984.tb00835.x. [DOI] [PubMed] [Google Scholar]
- Gossett K. A., MacWilliams P. S. Ultrastructure of canine toxic neutrophils. Am J Vet Res. 1982 Sep;43(9):1634–1637. [PubMed] [Google Scholar]
- Kanamori H., Press F. How thick is the lithosphere? Nature. 1970 Apr 25;226(5243):330–331. doi: 10.1038/226330a0. [DOI] [PubMed] [Google Scholar]
- Lichtman M. A., Weed R. I. Alteration of the cell periphery during granulocyte maturation: relationship to cell function. Blood. 1972 Mar;39(3):301–316. [PubMed] [Google Scholar]
- Marsh J. C., Boggs D. R., Cartwright G. E., Wintrobe M. M. Neutrophil kinetics in acute infection. J Clin Invest. 1967 Dec;46(12):1943–1953. doi: 10.1172/JCI105684. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCall C. E., Katayama I., Cotran R. S., Finland M. Lysosomal and ultrastructural changes in human "toxic" neutrophils during bacterial infection. J Exp Med. 1969 Feb 1;129(2):267–293. doi: 10.1084/jem.129.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Volkman A., Collins F. M. The cytokinetics of monocytosis in acute salmonella infection in the rat. J Exp Med. 1974 Feb 1;139(2):264–277. doi: 10.1084/jem.139.2.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss D. J., Krehbiel J. D., Lund J. E. Studies of the pathogenesis of anemia of inflammation: mechanism of impaired erythropoiesis. Am J Vet Res. 1983 Oct;44(10):1832–1835. [PubMed] [Google Scholar]