Table 3.
Comet data for determining DNA damage.
| Comet parameters | Control | 3.5 mg/mL Ca-ex | 7 mg/mL Ca-ex | 0.3 µg/L carbaryl | Carbaryl + 3.5 mg/mL Ca-ex | Carbaryl + 7 mg/mL Ca-ex |
|---|---|---|---|---|---|---|
| Head diameter (px) | 28.000 | 30.000 | 32.000 | 48.000 | 34.000 | 26.000 |
| Head density | 170.201 | 165.055 | 145.467 | 249.407 | 187.504 | 207.758 |
| Head DNA (%) | 97.5 ± 1.27a | 98.0 ± 1.15a | 98.5 ± 1.43a | 53.6 ± 1.51d | 62.5 ± 1.27c | 82.4 ± 1.71b |
| Tail length (px) | 4.000 | 3.000 | 1.000 | 71.000 | 59.000 | 22.000 |
| Tail density | 4.325 | 3.441 | 2.168 | 216.337 | 112.273 | 44.343 |
| Tail DNA (%) | 2.50 ± 1.27d | 2.00 ± 1.15d | 1.50 ± 1.43d | 46.4 ± 1.51a | 37.5 ± 1.27b | 17.6 ± 1.71c |
| Tail Moment | 0.280823 | 0.212094 | 0.184949 | 21.187 | 13.171 | 2.880 |
The data is displayed as mean ± SD (n = 10). 1,000 cells from each group were examined for DNA damage. In the same line, means denoted by various letters(a-d) are statistically significant (p < 0.05). The percentage of tail DNA served as the basis for the Comet scale, which indicated DNA damage. Damage can be classified as minimal or nonexistent (≤ 5%), low (5–20%), moderate (20–40%), high (40–75%), or severe (≥ 75%).