Table 1.
List of primers used for LAMP assay
| Primer ID | Primer type | Primer Sequence (5’−3’) | Length (nt) | Position | GC % | Target gene |
|---|---|---|---|---|---|---|
| F3 | Forward outer | TTCGACAGCCCCTATGCT | 18 | 67–84 | 56 | AV1 & AV2 gene |
| B3 | Backward outer | CCAATATGGGCCACGTCAT | 19 | 242–260 | 53 | |
| FIP (F1C + F2) | Forward inner |
CATGGGCCTGTTGGCCCATA CGTGCAGTTGTCCCCATTG |
39 |
88–106 128–147 |
58 | |
| BIP (B1C + B2) | Backward inner | GAAGCCCAGATGTACCACGGG GCCTGGACTCGAAAGACTG | 40 |
223–241 179–199 |
60 | |
| LF | Loop forward | TGCCTTTGTGCCTCGGA | 17 | 107–123 | 59 | |
| LB | Loop backward | GCTGTGAAGGCCCGTGT | 17 | 200–216 | 65 |