Correction for “Tumor-promoting UBR4 coordinates impaired mitophagy–associated senescence and lung adenocarcinoma pathogenesis,” by Dawon Jeong, Seo Hyeong Park, Jiwon Kim, Hyeyoon Kim, Yejin Jang, Jaemoon Koh, Yoon Kyung Jeon, Takafumi Tasaki, Yong Tae Kwon, Dohyun Han, Sung-Yup Cho, and Min Jae Lee, which published June 18, 2025; 10.1073/pnas.2425015122 (Proc. Natl. Acad. Sci. U.S.A. 122, e2425015122).
The authors note that Fig. 2 appeared incorrectly. Specifically, Fig. 2I mistakenly duplicated SI Appendix, Fig. S4F. The correct version of Fig. 2 and its legend appear below.
Fig. 2.
Impact of UBR4 deficiency on senescence phenotypes in cultured LUAD cells. (A) Relative colony formation rates in A549 WT and ΔUBR4 measured by crystal violet staining after low-density seeding. Dot plots with SD error bars from three independent experiments. (B) As in (A), except that an EdU incorporation assay was performed. (Left) Representative images display the proliferation of cells treated with EdU for 2 h, visualized under a confocal fluorescence microscope (green). (Right) Quantification of the percentage of EdU/DAPI-double- positive cells from ~5,000 cells, presented as box (interquartile ranges) and whisker plots (min to max). Individual cell surfaces are displayed in dotted lines. (Scale bar: 20 μm.) (C) Flow cytometry-based cell cycle analysis quantifying DNA content with DAPI staining. The proportions of WT and ΔUBR4 cells at each cell cycle phase based on flow cytometry histograms are summarized in the table. Values are shown in dot plots. (D) Representative images of WT and ΔUBR4 cells with IFS using anti-α-tubulin (green) antibodies with DAPI counterstaining. The percentage of cells with multiple nuclei was counted and presented as a box and whisker plot from three independent experiments with ∼200 cells. (E) Optical diffraction tomograms of WT and ΔUBR4 cells were taken using Tomocube microscopy, and volumes of whole-cell bodies and lipid droplets were quantified using the built-in software. (F) Representative images and quantification of enzymatic staining (pH 6.0) of senescence-associated β-galactosidase (SA-β-gal) in A549 WT and ΔUBR4 cells. Dot plots showing percentages of SA-β-gal positive cells. BF, bright field. (G) Lysosomal cargo delivery was monitored by dequenching of DQ-Green BSA fluorescence (FL; 10 μg/mL for 1 h) and DAPI counterstaining. Representative images and average DQ-Green positive intensities are presented as a box and whisker plot from three independent experiments. (Scale bar: 10 μm.) (H) Lysosomal activity in WT and ΔUBR4 cells was monitored by staining with LysoTracker (75 nM for 1 h). (Scale bars are 50 μm.) (I) Secreted human SASP factors from WT and ΔUBR4 cells were analyzed with the bead-based Luminex multiplex assay. ***P < 0.001 from unpaired, two-tailed Student’s t tests (N = 3).