Correction to Supporting Information for “Basolateral amygdala bidirectionally modulates stress-induced hippocampal learning and memory deficits through a p25/Cdk5-dependent pathway,” by Damien Rei, Xenos Mason, Jinsoo Seo, Johannes Gräff, Andrii Rudenko, Jun Wang, Richard Rueda, Sandra Siegert, Sukhee Cho, Rebecca G. Canter, Alison E. Mungenast, Karl Deisseroth, and Li-Huei Tsai, which published May 20, 2015; 10.1073/pnas.1415845112 (Proc. Natl. Acad. Sci. U.S.A. 112, 7291–7296).
The authors note that SI Appendix, Fig. S1G appeared incorrectly: “The β-Tub. blot from Fig. 1B was inadvertently duplicated in SI Appendix, Fig. S1G. We have identified the original films for the Western blots for both Fig. 1B and SI Appendix, Fig. S1G and confirmed the original synaptophysin (SYP) and β-Tub. blots depicted in SI Appendix, Fig. S1G were incorrect. We regret this error.”
The corrected SI Appendix, Fig. S1 appears below.
Fig. S1.
RFS-treated mice have normal locomotor and object exploration behavior. Restraint stress induces similar hippocampal molecular changes as those seen after RFS. The BLA did not show the molecular changes seen in the hippocampus after repeated stress. (A) RFS paradigm and timing of the novel object recognition (NO) and location recognition (NL) tasks (schematic). (B) Distance moved, velocity, and total exploration time in the novel object and novel location recognition tasks (unpaired t test, n = 8 per group). (C) Representative immunohistochemical images in dorsal hippocampal CA1 subregion and quantitative analysis of the effect of repeated stress on the expression levels of Synapsin II and Homer (HDAC2 in red; Synaptophysin, Synapsin II, and Homer in green; DAPI in blue) (unpaired t test, n = 4 per group). (D) qRT-PCR results of the effect of stress on the mRNA level of some known HDAC2 targets. The mRNA levels of these genes were significantly decreased in RFS-treated animals compared with controls. Other HDAC2 targets, such as NR2B and Neurexin, trended toward a decrease in the hippocampus of RFS-treated animals, but did not reach significance (unpaired t test, n = 7 per group). (E and F) Representative images and quantification of Synaptophysin (E) and HDAC2 (F) expression levels in the hippocampal CA3 subregion after RFS (unpaired t test, n = 3 per group) (Synaptophysin in green; HDAC2 in red). (G) Representative Western blot images and quantification of HDAC2, Synaptophysin, and p25 expression levels from control and restraint mice whole hippocampus lysates (unpaired t test, n = 5-6 per group). (H and I) Representative Western blot images and quantification of the effect of RFS on p25 (H), HDAC2, and Synaptophysin (I) levels in the BLA (unpaired t test, n = 5 per group). Values are mean ± SEM. n.s., nonsignificant; P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, unpaired t test. (Scale bars: 20 μm in C and 50 μm in E and F.)