Table 2.

Five primer groups with varying concentration and ratios

Primer name	Concentration of the Primer (µmol·L−1)
	Group 1	Group 2	Group 3	Group 4	Group 5
EV-G-F3/B3	0.2	0.2	0.2	0.2	0.2
EV-G-FIP/BIP	0.4	0.6	0.8	1.6	2.0
EV-G-LF	0.2	0.4	0.6	0.8	1.0