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. 2001 Jun;109(6):583–590. doi: 10.1289/ehp.01109583

Measurement of children's exposure to pesticides: analysis of urinary metabolite levels in a probability-based sample.

J L Adgate 1, D B Barr 1, C A Clayton 1, L E Eberly 1, N C Freeman 1, P J Lioy 1, L L Needham 1, E D Pellizzari 1, J J Quackenboss 1, A Roy 1, K Sexton 1
PMCID: PMC1240340  PMID: 11445512

Abstract

The Minnesota Children's Pesticide Exposure Study is a probability-based sample of 102 children 3-13 years old who were monitored for commonly used pesticides. During the summer of 1997, first-morning-void urine samples (1-3 per child) were obtained for 88% of study children and analyzed for metabolites of insecticides and herbicides: carbamates and related compounds (1-NAP), atrazine (AM), malathion (MDA), and chlorpyrifos and related compounds (TCPy). TCPy was present in 93% of the samples, whereas 1-NAP, MDA, and AM were detected in 45%, 37%, and 2% of samples, respectively. Measured intrachild means ranged from 1.4 microg/L for MDA to 9.2 microg/L for TCPy, and there was considerable intrachild variability. For children providing three urine samples, geometric mean TCPy levels were greater than the detection limit in 98% of the samples, and nearly half the children had geometric mean 1-NAP and MDA levels greater than the detection limit. Interchild variability was significantly greater than intrachild variability for 1-NAP (p = 0.0037) and TCPy (p < 0.0001). The four metabolites measured were not correlated within urine samples, and children's metabolite levels did not vary systematically by sex, age, race, household income, or putative household pesticide use. On a log scale, mean TCPy levels were significantly higher in urban than in nonurban children (7.2 vs. 4.7 microg/L; p = 0.036). Weighted population mean concentrations were 3.9 [standard error (SE) = 0.7; 95% confidence interval (CI), 2.5, 5.3] microg/L for 1-NAP, 1.7 (SE = 0.3; 95% CI, 1.1, 2.3) microg/L for MDA, and 9.6 (SE = 0.9; 95% CI, 7.8, 11) microg/L for TCPy. The weighted population results estimate the overall mean and variability of metabolite levels for more than 84,000 children in the census tracts sampled. Levels of 1-NAP were lower than reported adult reference range concentrations, whereas TCPy concentrations were substantially higher. Concentrations of MDA were detected more frequently and found at higher levels in children than in a recent nonprobability-based sample of adults. Overall, Minnesota children's TCPy and MDA levels were higher than in recent population-based studies of adults in the United States, but the relative magnitude of intraindividual variability was similar for adults and children.

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Selected References

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  1. Adgate J. L., Clayton C. A., Quackenboss J. J., Thomas K. W., Whitmore R. W., Pellizzari E. D., Lioy P. J., Shubat P., Stroebel C., Freeman N. C. Measurement of multi-pollutant and multi-pathway exposures in a probability-based sample of children: practical strategies for effective field studies. J Expo Anal Environ Epidemiol. 2000 Nov-Dec;10(6 Pt 2):650–661. doi: 10.1038/sj.jea.7500126. [DOI] [PubMed] [Google Scholar]
  2. Adgate J. L., Kukowski A., Stroebel C., Shubat P. J., Morrell S., Quackenboss J. J., Whitmore R. W., Sexton K. Pesticide storage and use patterns in Minnesota households with children. J Expo Anal Environ Epidemiol. 2000 Mar-Apr;10(2):159–167. doi: 10.1038/sj.jea.7500078. [DOI] [PubMed] [Google Scholar]
  3. Aprea C., Betta A., Catenacci G., Lotti A., Magnaghi S., Barisano A., Passini V., Pavan I., Sciarra G., Vitalone V. Reference values of urinary 3,5,6-trichloro-2-pyridinol in the Italian population--validation of analytical method and preliminary results (multicentric study). J AOAC Int. 1999 Mar-Apr;82(2):305–312. [PubMed] [Google Scholar]
  4. Aprea C., Strambi M., Novelli M. T., Lunghini L., Bozzi N. Biologic monitoring of exposure to organophosphorus pesticides in 195 Italian children. Environ Health Perspect. 2000 Jun;108(6):521–525. doi: 10.1289/ehp.00108521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beeson M. D., Driskell W. J., Barr D. B. Isotope dilution high-performance liquid chromatography/tandem mass spectrometry method for quantifying urinary metabolites of atrazine, malathion, and 2,4-dichlorophenoxyacetic acid. Anal Chem. 1999 Aug 15;71(16):3526–3530. doi: 10.1021/ac990130u. [DOI] [PubMed] [Google Scholar]
  6. Boeniger M. F., Lowry L. K., Rosenberg J. Interpretation of urine results used to assess chemical exposure with emphasis on creatinine adjustments: a review. Am Ind Hyg Assoc J. 1993 Oct;54(10):615–627. doi: 10.1080/15298669391355134. [DOI] [PubMed] [Google Scholar]
  7. Cohen Hubal E. A., Sheldon L. S., Burke J. M., McCurdy T. R., Berry M. R., Rigas M. L., Zartarian V. G., Freeman N. C. Children's exposure assessment: a review of factors influencing Children's exposure, and the data available to characterize and assess that exposure. Environ Health Perspect. 2000 Jun;108(6):475–486. doi: 10.1289/ehp.108-1638158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fenske R. A., Kissel J. C., Lu C., Kalman D. A., Simcox N. J., Allen E. H., Keifer M. C. Biologically based pesticide dose estimates for children in an agricultural community. Environ Health Perspect. 2000 Jun;108(6):515–520. doi: 10.1289/ehp.00108515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gurunathan S., Robson M., Freeman N., Buckley B., Roy A., Meyer R., Bukowski J., Lioy P. J. Accumulation of chlorpyrifos on residential surfaces and toys accessible to children. Environ Health Perspect. 1998 Jan;106(1):9–16. doi: 10.1289/ehp.981069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hill R. H., Jr, Ashley D. L., Head S. L., Needham L. L., Pirkle J. L. p-Dichlorobenzene exposure among 1,000 adults in the United States. Arch Environ Health. 1995 Jul-Aug;50(4):277–280. doi: 10.1080/00039896.1995.9935954. [DOI] [PubMed] [Google Scholar]
  11. Hill R. H., Jr, Head S. L., Baker S., Gregg M., Shealy D. B., Bailey S. L., Williams C. C., Sampson E. J., Needham L. L. Pesticide residues in urine of adults living in the United States: reference range concentrations. Environ Res. 1995 Nov;71(2):99–108. doi: 10.1006/enrs.1995.1071. [DOI] [PubMed] [Google Scholar]
  12. Hill R. H., Jr, Shealy D. B., Head S. L., Williams C. C., Bailey S. L., Gregg M., Baker S. E., Needham L. L. Determination of pesticide metabolites in human urine using an isotope dilution technique and tandem mass spectrometry. J Anal Toxicol. 1995 Sep;19(5):323–329. doi: 10.1093/jat/19.5.323. [DOI] [PubMed] [Google Scholar]
  13. Kutz F. W., Cook B. T., Carter-Pokras O. D., Brody D., Murphy R. S. Selected pesticide residues and metabolites in urine from a survey of the U.S. general population. J Toxicol Environ Health. 1992 Oct;37(2):277–291. doi: 10.1080/15287399209531670. [DOI] [PubMed] [Google Scholar]
  14. Landrigan P. J., Claudio L., Markowitz S. B., Berkowitz G. S., Brenner B. L., Romero H., Wetmur J. G., Matte T. D., Gore A. C., Godbold J. H. Pesticides and inner-city children: exposures, risks, and prevention. Environ Health Perspect. 1999 Jun;107 (Suppl 3):431–437. doi: 10.1289/ehp.99107s3431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lioy P. J., Edwards R. D., Freeman N., Gurunathan S., Pellizzari E., Adgate J. L., Quackenboss J., Sexton K. House dust levels of selected insecticides and a herbicide measured by the EL and LWW samplers and comparisons to hand rinses and urine metabolites. J Expo Anal Environ Epidemiol. 2000 Jul-Aug;10(4):327–340. doi: 10.1038/sj.jea.7500099. [DOI] [PubMed] [Google Scholar]
  16. Loewenherz C., Fenske R. A., Simcox N. J., Bellamy G., Kalman D. Biological monitoring of organophosphorus pesticide exposure among children of agricultural workers in central Washington State. Environ Health Perspect. 1997 Dec;105(12):1344–1353. doi: 10.1289/ehp.971051344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MacIntosh D. L., Needham L. L., Hammerstrom K. A., Ryan P. B. A longitudinal investigation of selected pesticide metabolites in urine. J Expo Anal Environ Epidemiol. 1999 Sep-Oct;9(5):494–501. doi: 10.1038/sj.jea.7500045. [DOI] [PubMed] [Google Scholar]
  18. O'Rourke M. K., Lizardi P. S., Rogan S. P., Freeman N. C., Aguirre A., Saint C. G. Pesticide exposure and creatinine variation among young children. J Expo Anal Environ Epidemiol. 2000 Nov-Dec;10(6 Pt 2):672–681. doi: 10.1038/sj.jea.7500119. [DOI] [PubMed] [Google Scholar]
  19. Quackenboss J. J., Pellizzari E. D., Shubat P., Whitmore R. W., Adgate J. L., Thomas K. W., Freeman N. C., Stroebel C., Lioy P. J., Clayton A. C. Design strategy for assessing multi-pathway exposure for children: the Minnesota Children's Pesticide Exposure Study (MNCPES). J Expo Anal Environ Epidemiol. 2000 Mar-Apr;10(2):145–158. doi: 10.1038/sj.jea.7500080. [DOI] [PubMed] [Google Scholar]
  20. Racke K. D. Environmental fate of chlorpyrifos. Rev Environ Contam Toxicol. 1993;131:1–150. doi: 10.1007/978-1-4612-4362-5_1. [DOI] [PubMed] [Google Scholar]
  21. Sexton K., Callahan M. A., Bryan E. F. Estimating exposure and dose to characterize health risks: the role of human tissue monitoring in exposure assessment. Environ Health Perspect. 1995 Apr;103 (Suppl 3):13–29. doi: 10.1289/ehp.95103s313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sexton K., Callahan M. A., Bryan E. F., Saint C. G., Wood W. P. Informed decisions about protecting and promoting public health: rationale for a National Human Exposure Assessment Survey. J Expo Anal Environ Epidemiol. 1995 Jul-Sep;5(3):233–256. [PubMed] [Google Scholar]
  23. Zartarian V. G., Ozkaynak H., Burke J. M., Zufall M. J., Rigas M. L., Furtaw E. J., Jr A modeling framework for estimating children's residential exposure and dose to chlorpyrifos via dermal residue contact and nondietary ingestion. Environ Health Perspect. 2000 Jun;108(6):505–514. doi: 10.1289/ehp.00108505. [DOI] [PMC free article] [PubMed] [Google Scholar]

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