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. 2001 Aug;109(8):859–864. doi: 10.1289/ehp.01109859

Predictors of airborne endotoxin in the home.

J H Park 1, D L Spiegelman 1, D R Gold 1, H A Burge 1, D K Milton 1
PMCID: PMC1240416  PMID: 11564624

Abstract

We identified home characteristics associated with the level of airborne endotoxin in 111 Boston-area homes enrolled in a cohort study of home exposures and childhood asthma, and we developed a predictive model to estimate airborne endotoxin. We measured endotoxin in family-room air and in dust from the baby's bed, family room, bedroom, and kitchen floor. Level of airborne endotoxin was weakly correlated (r < 0.3) with level of endotoxin in each of the four types of dust samples and was significantly correlated with endotoxin in family-room dust (p < 0.05). Endotoxin in family-room dust accounted for < 6% of the variability of airborne endotoxin. In a multivariate model, certain home characteristics were positively (p < 0.05) associated with airborne endotoxin. These included current presence of dog (difference in level, dog vs. no dog = 72%, partial R(2 )= 12.8%), past presence of dog (partial R(2) = 5.5%), and endotoxin level in family-room dust (partial R(2) = 5.3%). Use of a dehumidifier (partial R(2) = 6.4%) was negatively associated (p = 0.02; difference = -31%) with airborne endotoxin. Other home characteristics were identified as important determinants of increased airborne endotoxin in this model, but individual coefficients were not statistically significant (alpha = 0.05): total amount of fine dust collected in the home (partial R(2 )= 3.8%), concrete floor in family room (3.7%), water damage (3.6%), and use of cool-mist humidifier in past year (2.7%). This multivariate model explained 42% of the variability of airborne endotoxin levels, a substantial improvement over that with dust endotoxin alone. Airborne endotoxin in Boston-area homes appears to be determined by the presence of dogs, moisture sources, and increased amounts of settled dust.

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Selected References

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  1. Brunekreef B., Dockery D. W., Speizer F. E., Ware J. H., Spengler J. D., Ferris B. G. Home dampness and respiratory morbidity in children. Am Rev Respir Dis. 1989 Nov;140(5):1363–1367. doi: 10.1164/ajrccm/140.5.1363. [DOI] [PubMed] [Google Scholar]
  2. Castellan R. M., Olenchock S. A., Kinsley K. B., Hankinson J. L. Inhaled endotoxin and decreased spirometric values. An exposure-response relation for cotton dust. N Engl J Med. 1987 Sep 3;317(10):605–610. doi: 10.1056/NEJM198709033171005. [DOI] [PubMed] [Google Scholar]
  3. Christiani D. C., Ye T. T., Zhang S., Wegman D. H., Eisen E. A., Ryan L. A., Olenchock S. A., Pothier L., Dai H. L. Cotton dust and endotoxin exposure and long-term decline in lung function: results of a longitudinal study. Am J Ind Med. 1999 Apr;35(4):321–331. doi: 10.1002/(sici)1097-0274(199904)35:4<321::aid-ajim1>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  4. Gold D. R., Burge H. A., Carey V., Milton D. K., Platts-Mills T., Weiss S. T. Predictors of repeated wheeze in the first year of life: the relative roles of cockroach, birth weight, acute lower respiratory illness, and maternal smoking. Am J Respir Crit Care Med. 1999 Jul;160(1):227–236. doi: 10.1164/ajrccm.160.1.9807104. [DOI] [PubMed] [Google Scholar]
  5. Hasday J. D., Bascom R., Costa J. J., Fitzgerald T., Dubin W. Bacterial endotoxin is an active component of cigarette smoke. Chest. 1999 Mar;115(3):829–835. doi: 10.1378/chest.115.3.829. [DOI] [PubMed] [Google Scholar]
  6. Heederik D., Brouwer R., Biersteker K., Boleij J. S. Relationship of airborne endotoxin and bacteria levels in pig farms with the lung function and respiratory symptoms of farmers. Int Arch Occup Environ Health. 1991;62(8):595–601. doi: 10.1007/BF00381114. [DOI] [PubMed] [Google Scholar]
  7. Lindfors A., Wickman M., Hedlin G., Pershagen G., Rietz H., Nordvall S. L. Indoor environmental risk factors in young asthmatics: a case-control study. Arch Dis Child. 1995 Nov;73(5):408–412. doi: 10.1136/adc.73.5.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Michel O., Ginanni R., Duchateau J., Vertongen F., Le Bon B., Sergysels R. Domestic endotoxin exposure and clinical severity of asthma. Clin Exp Allergy. 1991 Jul;21(4):441–448. doi: 10.1111/j.1365-2222.1991.tb01684.x. [DOI] [PubMed] [Google Scholar]
  9. Michel O., Ginanni R., Le Bon B., Content J., Duchateau J., Sergysels R. Inflammatory response to acute inhalation of endotoxin in asthmatic patients. Am Rev Respir Dis. 1992 Aug;146(2):352–357. doi: 10.1164/ajrccm/146.2.352. [DOI] [PubMed] [Google Scholar]
  10. Michel O., Kips J., Duchateau J., Vertongen F., Robert L., Collet H., Pauwels R., Sergysels R. Severity of asthma is related to endotoxin in house dust. Am J Respir Crit Care Med. 1996 Dec;154(6 Pt 1):1641–1646. doi: 10.1164/ajrccm.154.6.8970348. [DOI] [PubMed] [Google Scholar]
  11. Milton D. K., Feldman H. A., Neuberg D. S., Bruckner R. J., Greaves I. A. Environmental endotoxin measurement: the Kinetic Limulus Assay with Resistant-parallel-line Estimation. Environ Res. 1992 Apr;57(2):212–230. doi: 10.1016/s0013-9351(05)80081-7. [DOI] [PubMed] [Google Scholar]
  12. Milton D. K., Johnson D. K., Park J. H. Environmental endotoxin measurement: interference and sources of variation in the Limulus assay of house dust. Am Ind Hyg Assoc J. 1997 Dec;58(12):861–867. doi: 10.1080/15428119791012199. [DOI] [PubMed] [Google Scholar]
  13. Park J. H., Spiegelman D. L., Burge H. A., Gold D. R., Chew G. L., Milton D. K. Longitudinal study of dust and airborne endotoxin in the home. Environ Health Perspect. 2000 Nov;108(11):1023–1028. doi: 10.1289/ehp.001081023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Platt S. D., Martin C. J., Hunt S. M., Lewis C. W. Damp housing, mould growth, and symptomatic health state. BMJ. 1989 Jun 24;298(6689):1673–1678. doi: 10.1136/bmj.298.6689.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rizzo M. C., Naspitz C. K., Fernández-Caldas E., Lockey R. F., Mimiça I., Solé D. Endotoxin exposure and symptoms in asthmatic children. Pediatr Allergy Immunol. 1997 Aug;8(3):121–126. doi: 10.1111/j.1399-3038.1997.tb00164.x. [DOI] [PubMed] [Google Scholar]
  16. Rylander R., Haglind P., Lundholm M., Mattsby I., Stenqvist K. Humidifier fever and endotoxin exposure. Clin Allergy. 1978 Sep;8(5):511–516. doi: 10.1111/j.1365-2222.1978.tb01504.x. [DOI] [PubMed] [Google Scholar]
  17. Saraf A., Larsson L., Burge H., Milton D. Quantification of ergosterol and 3-hydroxy fatty acids in settled house dust by gas chromatography-mass spectrometry: comparison with fungal culture and determination of endotoxin by a Limulus amebocyte lysate assay. Appl Environ Microbiol. 1997 Jul;63(7):2554–2559. doi: 10.1128/aem.63.7.2554-2559.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Saraf A., Park J. H., Milton D. K., Larsson L. Use of quadrupole GC-MS and ion trap GC-MS-MS for determining 3-hydroxy fatty acids in settled house dust: relation to endotoxin activity. J Environ Monit. 1999 Apr;1(2):163–168. doi: 10.1039/a809019j. [DOI] [PubMed] [Google Scholar]
  19. Schwartz D. A., Thorne P. S., Jagielo P. J., White G. E., Bleuer S. A., Frees K. L. Endotoxin responsiveness and grain dust-induced inflammation in the lower respiratory tract. Am J Physiol. 1994 Nov;267(5 Pt 1):L609–L617. doi: 10.1152/ajplung.1994.267.5.L609. [DOI] [PubMed] [Google Scholar]
  20. Schwartz D. A., Thorne P. S., Yagla S. J., Burmeister L. F., Olenchock S. A., Watt J. L., Quinn T. J. The role of endotoxin in grain dust-induced lung disease. Am J Respir Crit Care Med. 1995 Aug;152(2):603–608. doi: 10.1164/ajrccm.152.2.7633714. [DOI] [PubMed] [Google Scholar]
  21. Smid T., Heederik D., Houba R., Quanjer P. H. Dust- and endotoxin-related respiratory effects in the animal feed industry. Am Rev Respir Dis. 1992 Dec;146(6):1474–1479. doi: 10.1164/ajrccm/146.6.1474. [DOI] [PubMed] [Google Scholar]
  22. States B., Segal S. Levels of gamma-glutamyltranspeptidase in cultured skin fibroblasts from cystinotics and normals. Life Sci. 1980 Nov 24;27(21):1985–1990. doi: 10.1016/0024-3205(80)90419-1. [DOI] [PubMed] [Google Scholar]
  23. Svendsgaard D. J., Ward T. R., Tilson H. A., Kodavanti P. R. Empirical modeling of an in vitro activity of polychlorinated biphenyl congeners and mixtures. Environ Health Perspect. 1997 Oct;105(10):1106–1115. doi: 10.1289/ehp.971051106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vogelzang P. F., van der Gulden J. W., Folgering H., Kolk J. J., Heederik D., Preller L., Tielen M. J., van Schayck C. P. Endotoxin exposure as a major determinant of lung function decline in pig farmers. Am J Respir Crit Care Med. 1998 Jan;157(1):15–18. doi: 10.1164/ajrccm.157.1.9703087. [DOI] [PubMed] [Google Scholar]
  25. Von Ehrenstein O. S., Von Mutius E., Illi S., Baumann L., Böhm O., von Kries R. Reduced risk of hay fever and asthma among children of farmers. Clin Exp Allergy. 2000 Feb;30(2):187–193. doi: 10.1046/j.1365-2222.2000.00801.x. [DOI] [PubMed] [Google Scholar]
  26. Williamson I. J., Martin C. J., McGill G., Monie R. D., Fennerty A. G. Damp housing and asthma: a case-control study. Thorax. 1997 Mar;52(3):229–234. doi: 10.1136/thx.52.3.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yazicioğlu M., Saltik A., Oneş U., Sam A., Ekerbiçer H. C., Kirçuval O. Home environment and asthma in school children from the Edirne region in Turkey. Allergol Immunopathol (Madr) 1998 Jan-Feb;26(1):5–8. [PubMed] [Google Scholar]
  28. von Mutius E., Braun-Fahrländer C., Schierl R., Riedler J., Ehlermann S., Maisch S., Waser M., Nowak D. Exposure to endotoxin or other bacterial components might protect against the development of atopy. Clin Exp Allergy. 2000 Sep;30(9):1230–1234. doi: 10.1046/j.1365-2222.2000.00959.x. [DOI] [PubMed] [Google Scholar]

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