Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Aug 12;36(9):ar111. doi: 10.1091/mbc.E25-02-0057

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2025 Alabi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution 4.0 International Creative Commons CC-BY 4.0 License (https://creativecommons.org/licenses/by/4.0/).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 3: — LB1 depletion induces immediate lamin A/C meshwork disorganization. (A) Immunofluorescence of mNeonGreen and lamin A/C after 0, 2, 4, 8, and 16 h of auxin treatment in mNG-AID-LB1 cells; scale bar, 10 µm. (B) Normalized mNeonGreen intensity after 0, 2, 4, 8, and 16 h of auxin treatment in mNG-AID-LB1 cells. (C) The percentage of nuclei with lamin A/C defects detected by blinded categorical scoring after 0, 2, 4, 8, and 16 h of auxin treatment in mNG-AID-LB1 cells. In B and C, ns indicates p > 0.05; *, p < 0.05; ***, p < 0.001 by one-way ANOVA followed by Dunnett's multiple comparisons test. Average values of three independent replicate experiments shown; n > 350 cells analyzed per condition. (D) Representative masks of lamin A/C immunostaining in mNG-AID-LB1 cells after 0 h (left) or 16 h (right) of auxin treatment. (E) Summary plot of the percentage of nuclei with contiguous lamin A/C (<1% lamin A/C-free nuclear surface), with gaps in the lamin A/C meshwork covering >1% of the nuclear surface (orange), or with gaps covering >10% of the nuclear surface (red) in mNG-AID-LB1 cells after 0 h (n = 542) or 16 h (n = 522) auxin treatment. ****, p < 0.0001 by the Fisher's exact test. (F) Normalized histograms of the percentage of lamin A/C-free nuclear surface area in mNG-AID-LB1 cells after 0 h (n = 542) or 16 h (n = 522) auxin treatment. Curves indicate mean and error bars indicate SD of three independent replicate experiments. (G) Immunofluorescence of mNeonGreen and lamin A/C after 16 h of DMSO or auxin treatment in mNG-AID-LB2 cells. (H) Normalized mNeonGreen intensity after 16 h of DMSO or auxin treatment in mNG-AID-LB2 cells. (I) The percentage of nuclei with lamin A/C defects detected by blinded categorical scoring after 16 h of DMSO or auxin treatment in mNG-AID-LB2 cells. In H and I, ***, p < 0.001 and ns indicates p > 0.05 by the pairwise t test. n > 300 cells analyzed per condition in three independent replicate experiments.