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. 2025 Aug 12;36(9):ar111. doi: 10.1091/mbc.E25-02-0057

FIGURE 5:

FIGURE 5:

Neither Lamin A/C nor LB1 depletion are sufficient to cause nuclear rupture. (A and B) Still images from time-lapse imaging of mNG-AID-LA/C cells stably expressing 3xNLS-tdTomato and treated with DMSO vehicle control (A) or with auxin (B) for 16 h. (C) Quantification of NE rupture in mNG-AID-LA/C cells after 16 h of DMSO or auxin treatment. Average values for replicate experiments shown; n > 400 cells analyzed across three replicate experiments. (D and E) Still images from time-lapse imaging of mNG-AID-LB1 cells expressing 3xNLS-tdTomato and treated with DMSO vehicle control (D) or with auxin (E) for 16 h; scale bar, 10 µm. (F) Quantification of NE rupture in mNG-AID-LB1 cells after 16 h of DMSO or auxin treatment. Average values for replicate experiments shown; n > 600 cells analyzed across three replicate experiments.