1.
S. Weske, M. K. Nowak, and A. Zaufel, et al., “Intracellular Sphingosine‐1‐Phosphate Induces Lipolysis Through Direct Activation of Protein Kinase C Zeta,” FASEB Journal. 39, no. 7 (2025): e70528, https://doi.org/10.1096/fj.202403272R.
In the originally published version of this article, Figures 1 and 2 were mistakenly swapped. Accordingly, the figure legends must also be reassigned: the legend originally published for Figure 1 should be assigned to Figure 2 and vice versa. In addition, the legend for Figure 2, panels (E)‐(J), including the statistical information, pertains to Figure 1.
FIGURE 1.
S1P induces lipolysis through PKCzeta in differentiated 3T3‐L1 adipocytes. (A) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with S1P (0.1, 1, 10 or 50 μM) or vehicle controls (0.1% MeOH or 0.02% BSA) (n = 4 per group). (B) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with 10 μM S1P, 10 μM AUY954, 10 μM CYM5520, 10 μM CYM5541, or vehicle control (0.1% DMSO). (n = 4–5 per group). (C) Adipocyte‐associated S1P, sphingosine, and ceramide in 3T3‐L1 adipocytes after a 30 min incubation with 10 μM S1P as measured by LC–MS/MS and calculated based on an average cell volume of 4380 μm3. (n = 5 per group). (D) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with 10 μM S1P or vehicle control (0.02% BSA) in the presence or absence of the 25 μM ceramide synthase inhibitor Fumonisin B1. (n = 5 per group). (E) Gylcerol release per mg protein after 16h treatment of differentiated 3T3‐L1 cells with vehicle (0.02% BSA) vs 10 μM S1P or vehicle (0.1% DMSO) versus 1 μM Isoproterenol in the presence or absence of 100 μM of the adenylate cyclase inhibitor SQ22536. (n = 4 per group). (F) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with 10 μM S1P or vehicle control (0.02% BSA) in presence or absence of the 100 nM PI3K Inhibitor Wortmannin. (n = 3–8 per group). (G) Relative kinase activity of recombinant PKC zeta treated with 10 μM S1P or vehicle (MeOH) for 10 min as determined by in vitro phosphorylation assay. (n = 3 per group). (H) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with 10 μM S1P or vehicle control (0.02% BSA) in presence or absence of the 3 μM PKC inhibitor bisindolylmaleimid I (Bis). (n = 3–8 per group). (I) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with 10 μM S1P or vehicle (0.02% BSA) in the presence or absence of 10 M PKC zeta pseudo‐substrate inhibitor (PSI). (n = 4 per group). (J) Representative Western Blots for T410 phosphorylated PKC zeta, total PKC zeta and GAPDH in differentiated 3T3‐L1 adipocytes treated with 10 μM S1P or vehicle (0.02% BSA) for 15 min. (n = 4 per condition). Quantification of phospho‐PKC zeta T410 normalized GAPDH. Data are presented as mean ± SEM. A one‐way ANOVA with Tukey's multiple‐comparisons test or paired/unpaired t‐test was used for statistical analysis. *p < 0.05; **p < 0.01; ***p < 0.001.
FIGURE 2.
S1P induces lipolysis through a PKCzeta/MAPK/HSL pathway in differentiated 3T3‐L1 cells. (A) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with vehicle (0.02% BSA), 10 μM S1P (in 0.02% BSA) in the presence or absence of 10 μM PD98059 or 25 μM small molecular HSL inhibitor (smHSLi). (n = 4 per group). (B) Representative Western Blots for p‐p44/42 MAPK and p‐HSL Ser660 in differentiated 3T3‐L1 adipocytes treated with 10 μM S1P or vehicle (0.02% BSA) in the presence or absence of 3 μM bisindolylmaleimide I (Bis) for 15 min (n = 4–5 per condition). Quantification of p‐p44/42 MAPK and p‐HSL Ser660 normalized to GAPDH. (C) Glycerol release per mg protein after 16 h treatment of differentiated 3T3‐L1 cells with vehicle or 1 μM S1P lyase inhibitor VD‐78. (n = 5 per group). (D) Glycerol release per mg protein after 16 h treatment of primary mouse adipocytes with vehicle or 1 μM S1P lyase inhibitor VD‐78 (n = 6 per group). Data are presented as mean ± SEM. A one‐way ANOVA with Tukey's multiple‐comparisons test or paired t‐test was used for statistical analysis. *p < 0.05; **p < 0.01.
We apologize for this error.
The corrected versions of Figures 1 and 2 and their corresponding legends are provided below.