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. 2004 Apr;112(5):583–588. doi: 10.1289/ehp.6552

Ambient endotoxin concentrations in PM10 from Southern California.

Linda Mueller-Anneling 1, Ed Avol 1, John M Peters 1, Peter S Thorne 1
PMCID: PMC1241925  PMID: 15064165

Abstract

Concentrations of endotoxin in urban air pollution have not previously been extensively characterized. We measured 24-hr levels of PM10 (particulate matter < 10 microm in aerodynamic diameter) and the associated endotoxin component once every 6 weeks for 1 year in 13 communities in Southern California. All the samples collected had detectable PM10 and endotoxin levels. The geometric mean PM10 was 34.6 microg/m3 [geometric SD (GSD), 2.1; range, 3.0-135]. By volume, the endotoxin geometric mean was 0.44 endotoxin units (EU)/m3 (GSD, 3.1; range, 0.03-5.44). Per unit material collected, the geometric mean of endotoxin collected was 13.6 EU/mg (GSD, 3.2; range, 0.7-96.8). No correlation was found between endotoxin concentrations and other ambient pollutants concurrently measured [ozone, nitrogen dioxide, total acids, or PM2.5 (particulate matter < 2.5 micro m in aerodynamic diameter]. PM10 and endotoxin concentrations were significantly correlated, most strongly in summer. Samples collected in more rural and agricultural areas had lower PM10 and mid-range endotoxin levels. The high desert and mountain communities had lower PM10 levels but endotoxin levels comparable with or higher than the rural agricultural sites. By volume, endotoxin levels were highest at sites downwind of Los Angeles, California, which were also the locations of highest PM10. Endotoxin concentrations measured in this study were all < 5.5 EU/m3, which is lower than recognized thresholds for acute adverse health effects for occupational exposures but in the same range as indoor household concentrations. This study provides the first extensive characterization of endotoxin concentration across a large metropolitan area in relation to PM10 and other pollutant monitoring, and supports the need for studies of the role of endotoxin in childhood asthma in urban settings.

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Selected References

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  1. Bonner J. C., Rice A. B., Lindroos P. M., O'Brien P. O., Dreher K. L., Rosas I., Alfaro-Moreno E., Osornio-Vargas A. R. Induction of the lung myofibroblast PDGF receptor system by urban ambient particles from Mexico City. Am J Respir Cell Mol Biol. 1998 Oct;19(4):672–680. doi: 10.1165/ajrcmb.19.4.3176. [DOI] [PubMed] [Google Scholar]
  2. Castellan R. M., Olenchock S. A., Kinsley K. B., Hankinson J. L. Inhaled endotoxin and decreased spirometric values. An exposure-response relation for cotton dust. N Engl J Med. 1987 Sep 3;317(10):605–610. doi: 10.1056/NEJM198709033171005. [DOI] [PubMed] [Google Scholar]
  3. Clapp W. D., Becker S., Quay J., Watt J. L., Thorne P. S., Frees K. L., Zhang X., Koren H. S., Lux C. R., Schwartz D. A. Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract. Am J Respir Crit Care Med. 1994 Sep;150(3):611–617. doi: 10.1164/ajrccm.150.3.8087327. [DOI] [PubMed] [Google Scholar]
  4. Dockery D. W. Epidemiologic evidence of cardiovascular effects of particulate air pollution. Environ Health Perspect. 2001 Aug;109 (Suppl 4):483–486. doi: 10.1289/ehp.01109s4483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donaldson K., MacNee W. Potential mechanisms of adverse pulmonary and cardiovascular effects of particulate air pollution (PM10). Int J Hyg Environ Health. 2001 Jul;203(5-6):411–415. doi: 10.1078/1438-4639-00059. [DOI] [PubMed] [Google Scholar]
  6. Donham K., Haglind P., Peterson Y., Rylander R., Belin L. Environmental and health studies of farm workers in Swedish swine confinement buildings. Br J Ind Med. 1989 Jan;46(1):31–37. doi: 10.1136/oem.46.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Douwes J., Pearce N., Heederik D. Does environmental endotoxin exposure prevent asthma? Thorax. 2002 Jan;57(1):86–90. doi: 10.1136/thorax.57.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Douwes J., Thorne P., Pearce N., Heederik D. Bioaerosol health effects and exposure assessment: progress and prospects. Ann Occup Hyg. 2003 Apr;47(3):187–200. doi: 10.1093/annhyg/meg032. [DOI] [PubMed] [Google Scholar]
  9. Douwes J., Versloot P., Hollander A., Heederik D., Doekes G. Influence of various dust sampling and extraction methods on the measurement of airborne endotoxin. Appl Environ Microbiol. 1995 May;61(5):1763–1769. doi: 10.1128/aem.61.5.1763-1769.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Douwes J., Zuidhof A., Doekes G., van der Zee S. C., Wouters I., Boezen M. H., Brunekreef B. (1-->3)-beta-D-glucan and endotoxin in house dust and peak flow variability in children. Am J Respir Crit Care Med. 2000 Oct;162(4 Pt 1):1348–1354. doi: 10.1164/ajrccm.162.4.9909118. [DOI] [PubMed] [Google Scholar]
  11. Fairley D. Daily mortality and air pollution in Santa Clara County, California: 1989-1996. Environ Health Perspect. 1999 Aug;107(8):637–641. doi: 10.1289/ehp.99107637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gauderman W. J., McConnell R., Gilliland F., London S., Thomas D., Avol E., Vora H., Berhane K., Rappaport E. B., Lurmann F. Association between air pollution and lung function growth in southern California children. Am J Respir Crit Care Med. 2000 Oct;162(4 Pt 1):1383–1390. doi: 10.1164/ajrccm.162.4.9909096. [DOI] [PubMed] [Google Scholar]
  13. Gauderman W. James, Gilliland G. Frank, Vora Hita, Avol Edward, Stram Daniel, McConnell Rob, Thomas Duncan, Lurmann Fred, Margolis Helene G., Rappaport Edward B. Association between air pollution and lung function growth in southern California children: results from a second cohort. Am J Respir Crit Care Med. 2002 Jul 1;166(1):76–84. doi: 10.1164/rccm.2111021. [DOI] [PubMed] [Google Scholar]
  14. Heinrich J., Gehring U., Douwes J., Koch A., Fahlbusch B., Bischof W., Wichmann H. E., INGA-Study Group Pets and vermin are associated with high endotoxin levels in house dust. Clin Exp Allergy. 2001 Dec;31(12):1839–1845. doi: 10.1046/j.1365-2222.2001.01220.x. [DOI] [PubMed] [Google Scholar]
  15. Imrich A., Ning Y. Y., Koziel H., Coull B., Kobzik L. Lipopolysaccharide priming amplifies lung macrophage tumor necrosis factor production in response to air particles. Toxicol Appl Pharmacol. 1999 Sep 1;159(2):117–124. doi: 10.1006/taap.1999.8731. [DOI] [PubMed] [Google Scholar]
  16. Imrich A., Ning Y., Kobzik L. Insoluble components of concentrated air particles mediate alveolar macrophage responses in vitro. Toxicol Appl Pharmacol. 2000 Sep 1;167(2):140–150. doi: 10.1006/taap.2000.9002. [DOI] [PubMed] [Google Scholar]
  17. Jagielo P. J., Thorne P. S., Kern J. A., Quinn T. J., Schwartz D. A. Role of endotoxin in grain dust-induced lung inflammation in mice. Am J Physiol. 1996 Jun;270(6 Pt 1):L1052–L1059. doi: 10.1152/ajplung.1996.270.6.L1052. [DOI] [PubMed] [Google Scholar]
  18. Jedrychowski W., Flak E., Mróz E. The adverse effect of low levels of ambient air pollutants on lung function growth in preadolescent children. Environ Health Perspect. 1999 Aug;107(8):669–674. doi: 10.1289/ehp.99107669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kline J. N., Cowden J. D., Hunninghake G. W., Schutte B. C., Watt J. L., Wohlford-Lenane C. L., Powers L. S., Jones M. P., Schwartz D. A. Variable airway responsiveness to inhaled lipopolysaccharide. Am J Respir Crit Care Med. 1999 Jul;160(1):297–303. doi: 10.1164/ajrccm.160.1.9808144. [DOI] [PubMed] [Google Scholar]
  20. Li X. Y., Gilmour P. S., Donaldson K., MacNee W. In vivo and in vitro proinflammatory effects of particulate air pollution (PM10). Environ Health Perspect. 1997 Sep;105 (Suppl 5):1279–1283. doi: 10.1289/ehp.97105s51279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lippmann M., Schlesinger R. B. Toxicological bases for the setting of health-related air pollution standards. Annu Rev Public Health. 2000;21:309–333. doi: 10.1146/annurev.publhealth.21.1.309. [DOI] [PubMed] [Google Scholar]
  22. McConnell R., Berhane K., Gilliland F., London S. J., Vora H., Avol E., Gauderman W. J., Margolis H. G., Lurmann F., Thomas D. C. Air pollution and bronchitic symptoms in Southern California children with asthma. Environ Health Perspect. 1999 Sep;107(9):757–760. doi: 10.1289/ehp.99107757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Menetrez M. Y., Foarde K. K., Ensor D. S. An analytical method for the measurement of nonviable bioaerosols. J Air Waste Manag Assoc. 2001 Oct;51(10):1436–1442. doi: 10.1080/10473289.2001.10464365. [DOI] [PubMed] [Google Scholar]
  24. Michel O., Duchateau J., Sergysels R. Effect of inhaled endotoxin on bronchial reactivity in asthmatic and normal subjects. J Appl Physiol (1985) 1989 Mar;66(3):1059–1064. doi: 10.1152/jappl.1989.66.3.1059. [DOI] [PubMed] [Google Scholar]
  25. Michel O., Kips J., Duchateau J., Vertongen F., Robert L., Collet H., Pauwels R., Sergysels R. Severity of asthma is related to endotoxin in house dust. Am J Respir Crit Care Med. 1996 Dec;154(6 Pt 1):1641–1646. doi: 10.1164/ajrccm.154.6.8970348. [DOI] [PubMed] [Google Scholar]
  26. Milton D. K., Wypij D., Kriebel D., Walters M. D., Hammond S. K., Evans J. S. Endotoxin exposure-response in a fiberglass manufacturing facility. Am J Ind Med. 1996 Jan;29(1):3–13. doi: 10.1002/(SICI)1097-0274(199601)29:1<3::AID-AJIM2>3.0.CO;2-V. [DOI] [PubMed] [Google Scholar]
  27. Monn C., Becker S. Cytotoxicity and induction of proinflammatory cytokines from human monocytes exposed to fine (PM2.5) and coarse particles (PM10-2.5) in outdoor and indoor air. Toxicol Appl Pharmacol. 1999 Mar 15;155(3):245–252. doi: 10.1006/taap.1998.8591. [DOI] [PubMed] [Google Scholar]
  28. Ning Y., Imrich A., Goldsmith C. A., Qin G., Kobzik L. Alveolar macrophage cytokine production in response to air particles in vitro: role of endotoxin. J Toxicol Environ Health A. 2000 Feb 11;59(3):165–180. doi: 10.1080/009841000156952. [DOI] [PubMed] [Google Scholar]
  29. Ostro B. D., Broadwin R., Lipsett M. J. Coarse and fine particles and daily mortality in the Coachella Valley, California: a follow-up study. J Expo Anal Environ Epidemiol. 2000 Sep-Oct;10(5):412–419. doi: 10.1038/sj.jea.7500094. [DOI] [PubMed] [Google Scholar]
  30. Ostro B. D., Hurley S., Lipsett M. J. Air pollution and daily mortality in the Coachella Valley, California: a study of PM10 dominated by coarse particles. Environ Res. 1999 Oct;81(3):231–238. doi: 10.1006/enrs.1999.3978. [DOI] [PubMed] [Google Scholar]
  31. Park J. H., Gold D. R., Spiegelman D. L., Burge H. A., Milton D. K. House dust endotoxin and wheeze in the first year of life. Am J Respir Crit Care Med. 2001 Feb;163(2):322–328. doi: 10.1164/ajrccm.163.2.2002088. [DOI] [PubMed] [Google Scholar]
  32. Park J. H., Spiegelman D. L., Burge H. A., Gold D. R., Chew G. L., Milton D. K. Longitudinal study of dust and airborne endotoxin in the home. Environ Health Perspect. 2000 Nov;108(11):1023–1028. doi: 10.1289/ehp.001081023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Park J. H., Spiegelman D. L., Gold D. R., Burge H. A., Milton D. K. Predictors of airborne endotoxin in the home. Environ Health Perspect. 2001 Aug;109(8):859–864. doi: 10.1289/ehp.01109859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Peters J. M., Avol E., Gauderman W. J., Linn W. S., Navidi W., London S. J., Margolis H., Rappaport E., Vora H., Gong H., Jr A study of twelve Southern California communities with differing levels and types of air pollution. II. Effects on pulmonary function. Am J Respir Crit Care Med. 1999 Mar;159(3):768–775. doi: 10.1164/ajrccm.159.3.9804144. [DOI] [PubMed] [Google Scholar]
  35. Peters J. M., Avol E., Navidi W., London S. J., Gauderman W. J., Lurmann F., Linn W. S., Margolis H., Rappaport E., Gong H. A study of twelve Southern California communities with differing levels and types of air pollution. I. Prevalence of respiratory morbidity. Am J Respir Crit Care Med. 1999 Mar;159(3):760–767. doi: 10.1164/ajrccm.159.3.9804143. [DOI] [PubMed] [Google Scholar]
  36. Plopper C. G., Fanucchi M. V. Do urban environmental pollutants exacerbate childhood lung diseases? Environ Health Perspect. 2000 Jun;108(6):A252–A253. doi: 10.1289/ehp.108-a252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Pope C. A., 3rd, Bates D. V., Raizenne M. E. Health effects of particulate air pollution: time for reassessment? Environ Health Perspect. 1995 May;103(5):472–480. doi: 10.1289/ehp.95103472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Pope C. A., 3rd Mortality and air pollution: associations persist with continued advances in research methodology. Environ Health Perspect. 1999 Aug;107(8):613–614. doi: 10.1289/ehp.107-1566494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Rizzo M. C., Naspitz C. K., Fernández-Caldas E., Lockey R. F., Mimiça I., Solé D. Endotoxin exposure and symptoms in asthmatic children. Pediatr Allergy Immunol. 1997 Aug;8(3):121–126. doi: 10.1111/j.1399-3038.1997.tb00164.x. [DOI] [PubMed] [Google Scholar]
  40. Samet J. M., Dominici F., Curriero F. C., Coursac I., Zeger S. L. Fine particulate air pollution and mortality in 20 U.S. cities, 1987-1994. N Engl J Med. 2000 Dec 14;343(24):1742–1749. doi: 10.1056/NEJM200012143432401. [DOI] [PubMed] [Google Scholar]
  41. Samet J. M., Zeger S. L., Dominici F., Curriero F., Coursac I., Dockery D. W., Schwartz J., Zanobetti A. The National Morbidity, Mortality, and Air Pollution Study. Part II: Morbidity and mortality from air pollution in the United States. Res Rep Health Eff Inst. 2000 Jun;94(Pt 2):5–79. [PubMed] [Google Scholar]
  42. Schwartz D. A., Thorne P. S., Yagla S. J., Burmeister L. F., Olenchock S. A., Watt J. L., Quinn T. J. The role of endotoxin in grain dust-induced lung disease. Am J Respir Crit Care Med. 1995 Aug;152(2):603–608. doi: 10.1164/ajrccm.152.2.7633714. [DOI] [PubMed] [Google Scholar]
  43. Soukup J. M., Becker S. Human alveolar macrophage responses to air pollution particulates are associated with insoluble components of coarse material, including particulate endotoxin. Toxicol Appl Pharmacol. 2001 Feb 15;171(1):20–26. doi: 10.1006/taap.2000.9096. [DOI] [PubMed] [Google Scholar]
  44. Thorne P. S. Inhalation toxicology models of endotoxin- and bioaerosol-induced inflammation. Toxicology. 2000 Nov 2;152(1-3):13–23. doi: 10.1016/s0300-483x(00)00287-0. [DOI] [PubMed] [Google Scholar]
  45. Wouters I. M., Douwes J., Doekes G., Thorne P. S., Brunekreef B., Heederik D. J. Increased levels of markers of microbial exposure in homes with indoor storage of organic household waste. Appl Environ Microbiol. 2000 Feb;66(2):627–631. doi: 10.1128/aem.66.2.627-631.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Zock J. P., Hollander A., Heederik D., Douwes J. Acute lung function changes and low endotoxin exposures in the potato processing industry. Am J Ind Med. 1998 Apr;33(4):384–391. doi: 10.1002/(sici)1097-0274(199804)33:4<384::aid-ajim9>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]

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