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. 1993 Jun;217(6):634–643. doi: 10.1097/00000658-199306000-00005

Elemental diet-induced bacterial translocation can be hormonally modulated.

Y Haskel 1, D Xu 1, Q Lu 1, E Deitch 1
PMCID: PMC1242867  PMID: 8099475

Abstract

BACKGROUND: The authors have previously documented that feeding mice an elemental diet resulted in bacterial translocation (BT) that could be prevented by the provision of dietary fiber. To test whether the protective effect of fiber was related to the stimulation of trophic gut hormones, the effects of sandostatin and bombesin were tested. METHODS: Mice fed either chow or the elemental diet were stratified into several groups and the ability of bombesin (10 micrograms/kg, tid) or sandostatin (100 micrograms/kg bid) to modulate BT was examined. After 14 days, mice were sacrificed and BT, cecal bacterial population levels, mucosal protein, and small bowel weight was measured. Segments of the ileum and jejunum were examined histologically. RESULTS: Incidence of elemental diet-induced BT (75%) was reduced by fiber (9%) or the administration of bombesin (13%) (p < 0.01). Although sandostatin did not promote BT in chow-fed mice, it reversed the protective effect of fiber on BT (75%) (p < 0.01). CONCLUSION: Elemental diet-induced bacterial translocation can be modulated hormonally and the beneficial effects of fiber on diet-induced BT appears to be hormonally mediated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. W., MacMillan B. G., Stinnett J. D., Ogle C. K., Bozian R. C., Fischer J. E., Oakes J. B., Morris M. J., Krummel R. Beneficial effects of aggressive protein feeding in severely burned children. Ann Surg. 1980;192(4):505–517. doi: 10.1097/00000658-198010000-00009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alverdy J. C., Aoys E., Moss G. S. Total parenteral nutrition promotes bacterial translocation from the gut. Surgery. 1988 Aug;104(2):185–190. [PubMed] [Google Scholar]
  3. Alverdy J., Chi H. S., Sheldon G. F. The effect of parenteral nutrition on gastrointestinal immunity. The importance of enteral stimulation. Ann Surg. 1985 Dec;202(6):681–684. doi: 10.1097/00000658-198512000-00003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bass B. L., Fischer B. A., Richardson C., Harmon J. W. Somatostatin analogue treatment inhibits post-resectional adaptation of the small bowel in rats. Am J Surg. 1991 Jan;161(1):107–112. doi: 10.1016/0002-9610(91)90369-o. [DOI] [PubMed] [Google Scholar]
  5. Berg R. D., Garlington A. W. Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect Immun. 1979 Feb;23(2):403–411. doi: 10.1128/iai.23.2.403-411.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  7. Burke D. J., Alverdy J. C., Aoys E., Moss G. S. Glutamine-supplemented total parenteral nutrition improves gut immune function. Arch Surg. 1989 Dec;124(12):1396–1399. doi: 10.1001/archsurg.1989.01410120042009. [DOI] [PubMed] [Google Scholar]
  8. Chandra R. K. Nutrition, immunity, and infection: present knowledge and future directions. Lancet. 1983 Mar 26;1(8326 Pt 1):688–691. doi: 10.1016/s0140-6736(83)91980-3. [DOI] [PubMed] [Google Scholar]
  9. Chiu C. J., McArdle A. H., Brown R., Scott H. J., Gurd F. N. Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg. 1970 Oct;101(4):478–483. doi: 10.1001/archsurg.1970.01340280030009. [DOI] [PubMed] [Google Scholar]
  10. Debas H. T., Mulvihill S. J. Neuroendocrine design of the gut. Am J Surg. 1991 Feb;161(2):243–249. doi: 10.1016/0002-9610(91)91139-a. [DOI] [PubMed] [Google Scholar]
  11. Deitch E. A., Ma W. J., Ma L., Berg R. D., Specian R. D. Protein malnutrition predisposes to inflammatory-induced gut-origin septic states. Ann Surg. 1990 May;211(5):560–568. doi: 10.1097/00000658-199005000-00006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Deitch E. A., Maejima K., Berg R. Effect of oral antibiotics and bacterial overgrowth on the translocation of the GI tract microflora in burned rats. J Trauma. 1985 May;25(5):385–392. doi: 10.1097/00005373-198505000-00002. [DOI] [PubMed] [Google Scholar]
  13. Deitch E. A. Multiple organ failure. Pathophysiology and potential future therapy. Ann Surg. 1992 Aug;216(2):117–134. doi: 10.1097/00000658-199208000-00002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Deitch E. A., Winterton J., Li M., Berg R. The gut as a portal of entry for bacteremia. Role of protein malnutrition. Ann Surg. 1987 Jun;205(6):681–692. doi: 10.1097/00000658-198706000-00010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Evers B. M., Izukura M., Townsend C. M., Jr, Uchida T., Thompson J. C. Differential effects of gut hormones on pancreatic and intestinal growth during administration of an elemental diet. Ann Surg. 1990 May;211(5):630–638. [PMC free article] [PubMed] [Google Scholar]
  16. Evers B. M., Izukura M., Townsend C. M., Jr, Uchida T., Thompson J. C. Neurotensin prevents intestinal mucosal hypoplasia in rats fed an elemental diet. Dig Dis Sci. 1992 Mar;37(3):426–431. doi: 10.1007/BF01307738. [DOI] [PubMed] [Google Scholar]
  17. Fong Y. M., Marano M. A., Moldawer L. L., Wei H., Calvano S. E., Kenney J. S., Allison A. C., Cerami A., Shires G. T., Lowry S. F. The acute splanchnic and peripheral tissue metabolic response to endotoxin in humans. J Clin Invest. 1990 Jun;85(6):1896–1904. doi: 10.1172/JCI114651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Goodlad R. A., Lenton W., Ghatei M. A., Adrian T. E., Bloom S. R., Wright N. A. Proliferative effects of 'fibre' on the intestinal epithelium: relationship to gastrin, enteroglucagon and PYY. Gut. 1987;28 (Suppl):221–226. doi: 10.1136/gut.28.suppl.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Goodlad R. A., Wright N. A. Effects of addition of kaolin or cellulose to an elemental diet on intestinal cell proliferation in the mouse. Br J Nutr. 1983 Jul;50(1):91–98. doi: 10.1079/bjn19830075. [DOI] [PubMed] [Google Scholar]
  20. Hosoda N., Nishi M., Nakagawa M., Hiramatsu Y., Hioki K., Yamamoto M. Structural and functional alterations in the gut of parenterally or enterally fed rats. J Surg Res. 1989 Aug;47(2):129–133. doi: 10.1016/0022-4804(89)90076-0. [DOI] [PubMed] [Google Scholar]
  21. Jin G. F., Guo Y. S., Houston C. W. Bombesin: an activator of specific Aeromonas antibody secretion in rat intestine. Dig Dis Sci. 1989 Nov;34(11):1708–1712. doi: 10.1007/BF01540048. [DOI] [PubMed] [Google Scholar]
  22. Kudsk K. A., Croce M. A., Fabian T. C., Minard G., Tolley E. A., Poret H. A., Kuhl M. R., Brown R. O. Enteral versus parenteral feeding. Effects on septic morbidity after blunt and penetrating abdominal trauma. Ann Surg. 1992 May;215(5):503–513. doi: 10.1097/00000658-199205000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kudsk K. A., Stone J. M., Carpenter G., Sheldon G. F. Enteral and parenteral feeding influences mortality after hemoglobin-E. coli peritonitis in normal rats. J Trauma. 1983 Jul;23(7):605–609. doi: 10.1097/00005373-198307000-00010. [DOI] [PubMed] [Google Scholar]
  24. Lim G. M., Sheldon G. F., Alverdy J. Biliary secretory IgA levels in rats with protein-calorie malnutrition. Ann Surg. 1988 May;207(5):635–640. doi: 10.1097/00000658-198805000-00018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Lucey M. R., Yamada T. Biochemistry and physiology of gastrointestinal somatostatin. Dig Dis Sci. 1989 Mar;34(3 Suppl):5S–13S. doi: 10.1007/BF01536041. [DOI] [PubMed] [Google Scholar]
  26. Mochizuki H., Trocki O., Dominioni L., Brackett K. A., Joffe S. N., Alexander J. W. Mechanism of prevention of postburn hypermetabolism and catabolism by early enteral feeding. Ann Surg. 1984 Sep;200(3):297–310. doi: 10.1097/00000658-198409000-00007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Moore F. A., Moore E. E., Jones T. N., McCroskey B. L., Peterson V. M. TEN versus TPN following major abdominal trauma--reduced septic morbidity. J Trauma. 1989 Jul;29(7):916–923. doi: 10.1097/00005373-198907000-00003. [DOI] [PubMed] [Google Scholar]
  28. Mulholland M. W., Delaney J. P. Effects of low amine diet on gastric endocrine cell proliferation in the rat. J Surg Res. 1988 Aug;45(2):215–221. doi: 10.1016/0022-4804(88)90067-4. [DOI] [PubMed] [Google Scholar]
  29. Radulovic S. S., Milovanovic S. R., Cai R. Z., Schally A. V. The binding of bombesin and somatostatin and their analogs to human colon cancers. Proc Soc Exp Biol Med. 1992 Jul;200(3):394–401. doi: 10.3181/00379727-200-43447. [DOI] [PubMed] [Google Scholar]
  30. Spaeth G., Berg R. D., Specian R. D., Deitch E. A. Food without fiber promotes bacterial translocation from the gut. Surgery. 1990 Aug;108(2):240–247. [PubMed] [Google Scholar]
  31. Spaeth G., Specian R. D., Berg R. D., Deitch E. A. Bulk prevents bacterial translocation induced by the oral administration of total parenteral nutrition solution. JPEN J Parenter Enteral Nutr. 1990 Sep-Oct;14(5):442–447. doi: 10.1177/0148607190014005442. [DOI] [PubMed] [Google Scholar]
  32. Tannock G. W., Savage D. C. Influences of dietary and environmental stress on microbial populations in the murine gastrointestinal tract. Infect Immun. 1974 Mar;9(3):591–598. doi: 10.1128/iai.9.3.591-598.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Thompson J. C. Humoral control of gut function. Am J Surg. 1991 Jan;161(1):6–18. doi: 10.1016/0002-9610(91)90354-g. [DOI] [PubMed] [Google Scholar]
  34. Townsend C. M., Jr, Beauchamp R. D., Singh P., Thompson J. C. Growth factors and intestinal neoplasms. Am J Surg. 1988 Mar;155(3):526–536. doi: 10.1016/s0002-9610(88)80128-4. [DOI] [PubMed] [Google Scholar]
  35. Vahouny G. V., Le T., Ifrim I., Satchithanandam S., Cassidy M. M. Stimulation of intestinal cytokinetics and mucin turnover in rats fed wheat bran or cellulose. Am J Clin Nutr. 1985 May;41(5):895–900. doi: 10.1093/ajcn/41.5.895. [DOI] [PubMed] [Google Scholar]
  36. Wilmore D. W., Smith R. J., O'Dwyer S. T., Jacobs D. O., Ziegler T. R., Wang X. D. The gut: a central organ after surgical stress. Surgery. 1988 Nov;104(5):917–923. [PubMed] [Google Scholar]

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