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. 1961 Sep;80(3):578–584. doi: 10.1042/bj0800578

The isolation of the α-toxin of Clostridium welchii type A by zone electrophoresis in vertical columns

J Stephen 1
PMCID: PMC1243271  PMID: 16748926

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAILIE M. J., MORTON R. K. Comparative properties of microsomes from cow's milk and from mammary gland. 2. Chemical composition. Biochem J. 1958 May;69(1):44–53. doi: 10.1042/bj0690044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BATTY I., BULLEN J. J. The effect of Clostridium welchii type D culture filtrates on the permeability of the mouse intestine. J Pathol Bacteriol. 1956 Apr;71(2):311–323. doi: 10.1002/path.1700710206. [DOI] [PubMed] [Google Scholar]
  3. BIDWELL E. Proteolytic enzymes of Clostridium welchii. Biochem J. 1950 May;46(5):589–598. doi: 10.1042/bj0460589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BULLEN J. J., THURLBOURN C., BROWN P. A large high-pressure ultrafilter. Nature. 1954 Feb 6;173(4397):254–255. doi: 10.1038/173254a0. [DOI] [PubMed] [Google Scholar]
  5. CONCHIE J., FINDLAY J., LEVVY G. A. Mammalian glycosidases; distribution in the body. Biochem J. 1959 Feb;71(2):318–325. doi: 10.1042/bj0710318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FLODIN P., KUPKE D. W. Zone electrophoresis on cellulose columns. Biochim Biophys Acta. 1956 Aug;21(2):368–376. doi: 10.1016/0006-3002(56)90021-x. [DOI] [PubMed] [Google Scholar]
  7. GARBERS C. F., JOUBERT F. J. Effect of the borate ion in buffers on the electrophoresis of rat serum. Nature. 1958 Aug 23;182(4634):530–531. doi: 10.1038/182530a0. [DOI] [PubMed] [Google Scholar]
  8. GRABAR P., WILLIAMS C. A., Jr Méthode immuno-électrophorétique d'analyse de mélanges de substances antigéniques. Biochim Biophys Acta. 1955 May;17(1):67–74. doi: 10.1016/0006-3002(55)90320-6. [DOI] [PubMed] [Google Scholar]
  9. GRABAR P., WILLIAMS C. A. Méthode permettant l'étude conjuguée des proprietés électrophorétiques et immunochimiques d'un mélange de protéines; application au sérum sanguin. Biochim Biophys Acta. 1953 Jan;10(1):193–194. doi: 10.1016/0006-3002(53)90233-9. [DOI] [PubMed] [Google Scholar]
  10. Gale E. F., Van Heyningen W. E. The effect of the pH and the presence of glucose during growth on the production of alpha and theta toxins and hyaluronidase by Clostridium welchii. Biochem J. 1942 Sep;36(7-9):624–630. doi: 10.1042/bj0360624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HABERMANN E. Uber die Giftkomponenten des Gasbranderregers Clostridium Welchii Ty. A. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1959;235(6):513–534. [PubMed] [Google Scholar]
  12. HOWIE J. W., DUNCAN I. B., MACKIE L. M. Growth of Clostridium welchii in the stomach after partial gastrectomy. Lancet. 1953 Nov 14;265(6794):1018–1021. doi: 10.1016/s0140-6736(53)91309-9. [DOI] [PubMed] [Google Scholar]
  13. KOHN J. A simple method for the concentration of fluids containing protein. Nature. 1959 Apr 11;183(4667):1055–1055. doi: 10.1038/1831055a0. [DOI] [PubMed] [Google Scholar]
  14. KUSHNER D. J. An evaluation of the egg-yolk reaction as a test for lecithinase activity. J Bacteriol. 1957 Mar;73(3):297–302. doi: 10.1128/jb.73.3.297-302.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. MACFARLANE M. G. The biochemistry of bacterial toxins; the lecithinase activity of Clostridium haemolyticum toxin. Biochem J. 1950 Sep;47(3):267–270. doi: 10.1042/bj0470267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Macfarlane M. G., Knight B. C. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins. Biochem J. 1941 Sep;35(8-9):884–902. doi: 10.1042/bj0350884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ROTH F. B., PILLEMER L. The separation of alpha toxin (lecithinase) from filtrates of Clostridium welchii. J Immunol. 1953 Jun;70(6):533–537. [PubMed] [Google Scholar]
  19. Van Heyningen W. E., Bidwell E. The biochemistry of the gas gangrene toxins: 4. The reaction between the alpha-toxin (lecithinase) of Clostridium welchii and its antitoxin. Biochem J. 1948;42(1):130–140. [PMC free article] [PubMed] [Google Scholar]
  20. Van Heyningen W. E. The biochemistry of the gas gangrene toxins: Estimation of the alpha toxin of Cl. welchii, type A. Biochem J. 1941 Nov;35(10-11):1246–1256. doi: 10.1042/bj0351246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van Heyningen W. E. The biochemistry of the gas gangrene toxins: Partial purification of the toxins of Cl. welchii, type A. Separation of alpha and theta toxins. Biochem J. 1941 Nov;35(10-11):1257–1269. doi: 10.1042/bj0351257. [DOI] [PMC free article] [PubMed] [Google Scholar]

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