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. 1961 Sep;80(3):597–605. doi: 10.1042/bj0800597

Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 2. The isolation, crystallization and chemical characterization of mouse encephalomyocarditis virus

P Faulkner 1, E M Martin 1, S Sved 1, R C Valentine 1, T S Work 1
PMCID: PMC1243273  PMID: 13698341

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Selected References

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  1. BOMAN H. G. Chromatography of prostatic phosphatase. Biochim Biophys Acta. 1955 Feb;16(2):245–253. doi: 10.1016/0006-3002(55)90210-9. [DOI] [PubMed] [Google Scholar]
  2. BREESE S. S., Jr, TRAUTMAN R. Examination of spray-droplet particle counting as a measure of virus concentration. Virology. 1960 Jan;10:57–72. doi: 10.1016/0042-6822(60)90006-4. [DOI] [PubMed] [Google Scholar]
  3. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  4. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FAULKNER P., MARTIN E. M., SVED S., WORK T. S. Crystallization of purified mouse encephalomyocarditis virus particles. Nature. 1960 Jun 11;186:908–909. doi: 10.1038/186908a0. [DOI] [PubMed] [Google Scholar]
  6. GESSLER A. E., BENDER C. E., PARKINSON M. C. A new and rapid method for isolating viruses by selective fluorocarbon deproteinization. Trans N Y Acad Sci. 1956 Jun;18(8):701–703. doi: 10.1111/j.2164-0947.1956.tb00497.x. [DOI] [PubMed] [Google Scholar]
  7. HOSKINS J. M., SANDERS F. K. Propagation of mouse encephalomyocarditis virus in ascites tumour cells maintained in vitro. Br J Exp Pathol. 1957 Jun;38(3):268–272. [PMC free article] [PubMed] [Google Scholar]
  8. HUMMELER K., HAMPARIAN V. Removal of anticomplementary activity and host antigens from viral preparations by fluorocarbon. Science. 1957 Mar 22;125(3247):547–548. doi: 10.1126/science.125.3247.547. [DOI] [PubMed] [Google Scholar]
  9. HURLBERT R. B., SCHMITZ H., BRUMM A. F., POTTER V. R. Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides. J Biol Chem. 1954 Jul;209(1):23–39. [PubMed] [Google Scholar]
  10. LEVINTOW L., DARNELL J. E., Jr A simplified procedure for purification of large amounts of poliovirus: characterization and amino acid analysis of Type 1 poliovirus. J Biol Chem. 1960 Jan;235:70–73. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. LURIA S. E., WILLIAMS R. C., BACKUS R. C. Electron micrographic counts of bacteriophage particles. J Bacteriol. 1951 Feb;61(2):179–188. doi: 10.1128/jb.61.2.179-188.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MANSON L. A., ROTHSTEIN E. L., RAKE G. W. Purification of poliovirus with fluorocarbon. Science. 1957 Mar 22;125(3247):546–547. doi: 10.1126/science.125.3247.546-a. [DOI] [PubMed] [Google Scholar]
  14. MARKHAM R. Chromatography of nucleotides and related substances. Br Med Bull. 1954;10(3):214–217. doi: 10.1093/oxfordjournals.bmb.a069424. [DOI] [PubMed] [Google Scholar]
  15. MARTIN E. M., MALEC J., COOTE J. L., WORK T. S. Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 3. Methods for the disruption of Krebs II mouse-ascites-tumour cells. Biochem J. 1961 Sep;80:606–611. doi: 10.1042/bj0800606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MARTIN E. M., MALEC J., SVED S., WORK T. S. Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 1. Encephalomyocarditis virus in Krebs II mouse-ascites-tumour cells. Biochem J. 1961 Sep;80:585–597. doi: 10.1042/bj0800585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MARTIN E. M., MORTON R. K. The chemical composition of microsomes and mitochondria from silver beet. Biochem J. 1956 Oct;64(2):221–235. doi: 10.1042/bj0640221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PEREIRA M. S., PEREIRA H. G., ALLISON A. C. Use of gel diffusion precipitation test in the diagnosis of adenovirus infections. Lancet. 1959 Mar 14;1(7072):551–552. doi: 10.1016/s0140-6736(59)92311-6. [DOI] [PubMed] [Google Scholar]
  19. PORTERFIELD J. S., ROWE C. E. Hemagglutination with arthropod-borne viruses and its inhibition by certain phospholipids. Virology. 1960 Aug;11:765–770. doi: 10.1016/0042-6822(60)90119-7. [DOI] [PubMed] [Google Scholar]
  20. SANDERS F. K., SELLERS R. F., PAY T. W. Discussion: recent advances in the use of tissue culture methods in virus research. Proc R Soc Med. 1957 Oct;50(10):911–922. [PMC free article] [PubMed] [Google Scholar]
  21. SCHAFFER F. L., MOORE H. F., SCHWERDT C. E. Base composition of the ribonucleic acids of the three types of poliovirus. Virology. 1960 Apr;10:530–537. doi: 10.1016/0042-6822(60)90134-3. [DOI] [PubMed] [Google Scholar]
  22. SCHAFFER F. L., SCHWERDT C. E. Purification of poliomyelitis viruses propagated in tissue culture. Virology. 1956 Oct;2(5):665–678. doi: 10.1016/0042-6822(56)90046-0. [DOI] [PubMed] [Google Scholar]
  23. SHARP D. G. Purification and properties of animal viruses. Adv Virus Res. 1953;1:277–313. doi: 10.1016/s0065-3527(08)60466-2. [DOI] [PubMed] [Google Scholar]
  24. SMITH J. D., MARKHAM R. Chromatographic studies on nucleic acids; the quantitative analysis of ribonucleic acids. Biochem J. 1950 May;46(5):509–513. doi: 10.1042/bj0460509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. TAVERNE J., MARSHALL J. H., FULTON F. The purification and concentration of viruses and virus soluble antigens on calcium phosphate. J Gen Microbiol. 1958 Dec;19(3):451–461. doi: 10.1099/00221287-19-3-451. [DOI] [PubMed] [Google Scholar]
  26. VALENTINE R. C. The shape of protein molecules suggested by electron microscopy. Nature. 1959 Dec 12;184:1838–1841. doi: 10.1038/1841838a0. [DOI] [PubMed] [Google Scholar]
  27. WEIL M. L., WARREN J., BREESE S. S., Jr, RUSS S. B., JEFFRIES H. Separation of encephalomyocarditis virus from tissue components by means of protamine precipitation and enzymic digestion. J Bacteriol. 1952 Jan;63(1):99–105. doi: 10.1128/jb.63.1.99-105.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]

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