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. 1981;319:53–64. doi: 10.1113/jphysiol.1981.sp013891

Effect of glucose and hyperosmolality on the electrical characteristics of dog colon mucosa in vivo.

S Gazitúa
PMCID: PMC1243821  PMID: 7320926

Abstract

1. A method has been developed to maintain the electrical characteristics of dog colon mucosa stable over prolonged periods in vivo. This was achieved by paying special attention to the constancy of the volume of the organ and its intraluminal pressure during perfusion. 2. Intraluminal glucose elicits an increase in transmural potential and short-circuit current; this has been attributed to the presence of a Na-glucose co-transport process in this tissue. 3. Na replacement by K in the perfusate caused an increase in the transmural potential, whereas mannitol substitution evoked an inversion. These observations are probably the result of diffusion potentials. In the mannitol solution, glucose effectively abolished the negative transmural potential, whereas in the K solution, the effect of the sugar was smaller than in the control. 4. Perfusion of a hyperosmotic solution containing mannitol resulted in the development of streaming potentials across the mucosa. Their magnitude was reduced by addition of 2,4-dinitrophenol or by removal of Na from the perfusate. They were counteracted by replacement of mannitol by glucose. The streaming potentials showed no sign of saturability, reflecting a pronounced hydraulic conductivity of the epithelium. 5. The rapidity of onset of the potential response to glucose was enhanced by increasing the perfusion rate, but the response to hyperosmolality was unaffected. 6. Streaming potentials, diffusion potentials and glucose-evoked potentials are generally considered to be features of the small intestinal mucosa; their existence in the dog colon indicates that this epithelium has more in common with small gut than with the majority of mammalian colons.

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Selected References

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  1. Armstrong W. M., Byrd B. J., Cohen E. S., Cohen S. J., Hamang P. H., Myers C. J. Osmotically induced electrical changes in isolated bullfrog small intestine. Biochim Biophys Acta. 1975 Aug 5;401(1):137–151. doi: 10.1016/0005-2736(75)90348-x. [DOI] [PubMed] [Google Scholar]
  2. BARRY R. J., DIKSTEIN S., MATTHEWS J., SMYTH D. H., WRIGHT E. M. ELECTRICAL POTENTIALS ASSOCIATED WITH INTESTINAL SUGAR TRANSFER. J Physiol. 1964 Jun;171:316–338. doi: 10.1113/jphysiol.1964.sp007379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crane R. K., Forstner G., Eichholz A. Studies on the mechanism of the intestinal absorption of sugars. X. An effect of Na+ concentration on the apparent Michaelis constants for intestinal sugar transport, in vitro. Biochim Biophys Acta. 1965 Nov 29;109(2):467–477. doi: 10.1016/0926-6585(65)90172-x. [DOI] [PubMed] [Google Scholar]
  4. Diamond J. M., Harrison S. C. The effect of membrane fixed charges on diffusion potentials and streaming potentials. J Physiol. 1966 Mar;183(1):37–57. doi: 10.1113/jphysiol.1966.sp007850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edmonds C. J., Marriott J. Sodium transport and short-circuit current in rat colon in vivo and the effect of aldosterone. J Physiol. 1970 Nov;210(4):1021–1039. doi: 10.1113/jphysiol.1970.sp009255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Garcia-Diaz J. F., Corcia A. Electrical changes in isolated rat jejunum induced by hypertonicity. Biochim Biophys Acta. 1977 Jul 14;468(2):177–187. doi: 10.1016/0005-2736(77)90112-2. [DOI] [PubMed] [Google Scholar]
  7. Levin R. J. Transmural potentials across the small and large intestine of the bullfrog, Rana catesbeiana. Proc Soc Exp Biol Med. 1966 Apr;121(4):1033–1038. doi: 10.3181/00379727-121-30957. [DOI] [PubMed] [Google Scholar]
  8. Loeschke K., Bentzel C. J., Csáky T. Z. Asymmetry of osmotic flow in frog intestine: functional and structural correlation. Am J Physiol. 1970 Jun;218(6):1723–1731. doi: 10.1152/ajplegacy.1970.218.6.1723. [DOI] [PubMed] [Google Scholar]
  9. Mateu L., Robinson J. W. Effect of different bathing media on the short-circuit current across the intestine of the rat and guinea-pig. Experientia. 1976 Jan 15;32(1):62–64. doi: 10.1007/BF01932624. [DOI] [PubMed] [Google Scholar]
  10. Rey F., Drillet F., Schmitz J., Rey J. Influence of flow rate on the kinetics of the intestinal absorption of glucose and lysine in children. Gastroenterology. 1974 Jan;66(1):79–85. [PubMed] [Google Scholar]
  11. Robinson J. W., Luisier A. L., Mirkovitch V. Transport of amino-acids and sugars by the dog colonic mucosa. Pflugers Arch. 1973;345(4):317–326. doi: 10.1007/BF00585850. [DOI] [PubMed] [Google Scholar]
  12. SCHULTZ S. G., ZALUSKY R. ION TRANSPORT IN ISOLATED RABBIT ILEUM. I. SHORT-CIRCUIT CURRENT AND NA FLUXES. J Gen Physiol. 1964 Jan;47:567–584. doi: 10.1085/jgp.47.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smyth D. H., Wright E. M. Streaming potentials in the rat small intestine. J Physiol. 1966 Feb;182(3):591–602. doi: 10.1113/jphysiol.1966.sp007839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilson F. A., Dietschy J. M. The intestinal unstirred layer: its surface area and effect on active transport kinetics. Biochim Biophys Acta. 1974 Aug 21;363(1):112–126. doi: 10.1016/0005-2736(74)90010-8. [DOI] [PubMed] [Google Scholar]
  15. Winne D., Kopf S., Ulmer M. L. Role of unstirred layer in intestinal absorption of phenylalanine in vivo. Biochim Biophys Acta. 1979 Jan 5;550(1):120–130. doi: 10.1016/0005-2736(79)90120-2. [DOI] [PubMed] [Google Scholar]
  16. Winne D. Unstirred layer, source of biased Michaelis constant in membrane transport. Biochim Biophys Acta. 1973 Feb 27;298(1):27–31. doi: 10.1016/0005-2736(73)90005-9. [DOI] [PubMed] [Google Scholar]

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