Corrigendum. Proposal of Lactobacillus amylovorus subsp. animalis subsp. nov. and an emended description of Lactobacillus amylovorus

In the published version of the article, we proposed the name Lactobacillus amylovorus subsp. animalis. This name is illegitimate, as it contravenes Rule 12(b) of the International Code of Nomenclature of Prokaryotes [1]. As a replacement name, we propose Lactobacillus amylovorus subsp. animalium. The protologue is then as follows:

Lactobacillus amylovorus subsp. animalium (a.ni.ma′li.um. L. gen. pl. n. animalium, of animals).

The bacterium is Gram-stain-positive, nonmotile, non-spore-forming, catalase-negative, cytochrome oxidase-negative, facultatively anaerobic, homofermentative and rod-shaped. The bacteria are 0.5–0.7 µm in width and 3.0–7.0 µm in length. Colonies grown on de Man, Rogosa, and Sharpe (MRS; Difco, Detroit, MI, USA) agar plates at 37 °C for 3 days under anaerobic conditions are 1.0–2.0 mm in diameter and milky white, round and glossy in appearance. This strain does not produce any gas from glucose; both d- and l-lactate are produced as end products of glucose (d/l, 40 : 60). Growth occurs at a temperature of 30–45 °C (optimum 37 °C), pH of 5.0–8.0 (optimum pH 6.0) and NaCl concentration of 1.0–3.0% (w/v). In the API 50 CH test system (incubation at 37 °C for 2 days), acids are produced from d-glucose, d-fructose, N-acetyl-glucosamine, aesculin ferric citrate, d-maltose, d-lactose, d-sucrose and starch, but not from glycerol, erythritol, d-arabinose, l-arabinose, d-ribose, d-xylose, l-xylose, d-adonitol, methyl-β-d-xylopyranoside, l-sorbose, l-rhamnose, dulcitol, inositol, d-mannitol, d-sorbitol, methyl-α-d-mannopyranoside, methyl-α-d-glucopyranoside, amygdalin, arbutin, salicin, d-melibiose, inulin, d-melezitose, glycogen, xylitol, gentiobiose, d-turanose, d-lyxose, d-tagatose, d-fucose, l-fucose, d-arabitol, l-arabitol, gluconate, 2-keto-gluconate and 5-keto-gluconate. Variable reactions have been observed for acid production from d-galactose, d-mannose, d-cellobiose, d-trehalose and d-raffinose. For this proposed subspecies, aesculin citrate is the only β-glucoside that supports acid formation, but citrate is also a substrate for the metabolism of many lactobacilli. In the API ZYM test system, positive reactions are obtained from esterase (C4), leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β-galactosidase, α-glucosidase and β-glucosidase. Negative reactions are obtained from alkaline phosphatase, lipase (C14), trypsin, α-chymotrypsin, β-glucuronidase, N-acetyl-β-glucosaminidase, α-mannosidase and α-fucosidase. The reactions of the following enzymes vary: esterase lipase (C8) and α-galactosidase. Dextran is not produced from sucrose, and ammonia is not produced from arginine. C_{16 : 0}, C_{18 : 1} ω9c, C₁₉ cyclopropane 9,10 and summed feature 10 are the major fatty acids in strain BF125^T. The genome size of the type strain is 1.98 Mbp, and the mol% G+C content of the DNA is 37.8 (whole-genome analysis). The type strain BF125^T (= MAFF 212522^T = DSM 115528^T) is isolated from bovine faeces. At least five additional strains [BF186, TKL145, Bifido-178-WT-3C (= DSM 107288), S60, AF08-3, and DSM 16698] are included in this subspecies. The GenBank/The European Molecular Biology Laboratory (EMBL) /DNA Data Bank of Japan (DDBJ) accession number for the 16S rRNA gene sequence of strain BF125^T is LC771959. The International Nucleotide Sequence Database Collaboration (INSDC) accession numbers for the draft genome sequence of strain BF125^T are BTFR01000001−BTFR01000033.

We would like to thank Mr Meng-Syun Li (National Chung Hsing University) and Dr Aharon Oren (The Hebrew University of Jerusalem) for their contribution to this corrigendum. The authors apologize for any inconvenience caused.