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. 1981 Jul;316:33–46. doi: 10.1113/jphysiol.1981.sp013770

The effect of tetraethylammonium chloride on potassium permeability in the smooth muscle cell membrane of canine trachea.

Y Imaizumi, M Watanabe
PMCID: PMC1248134  PMID: 7320871

Abstract

1. The effect of tetraethylammonium ions (TEA) on potassium or rubidium permeability was studied in canine tracheal smooth muscle. 2. TEA (15-30 mM) markedly increased the rate of 42K- and 86Rb-efflux in normal Krebs solution. This increase is probably due mainly to the occurrence of electrical activity such as spike potentials and only partially to depolarization. 3. The rate coefficients of 42K- and 86Rb-efflux from depolarized tracheal smooth muscle bathed in a medium with elevated potassium (50-100 mM) were so large that the coefficients did not remain constant. When chloride ions in the medium were replaced with larger anions such as acetate, propionate or benzoate, the rate of 86Rb-efflux remained constant even in high-potassium solution (70 mM). 4. TEA caused a remarkable blockade of 86Rb-efflux in depolarized tracheal smooth muscle. The maximum decrease in the rate coefficient by TEA was approximately 52.5% of the control value in high-potassium-acetate solution. The dissociation constant for the interaction between TEA and its sites of action on the cell membrane was about 0.93 mM. 5. Spontaneous activity was, however, elicited only when TEA was added at a concentration of 10-30 mM and the membrane was depolarized more than 15-20 mV. It is assumed that TEA blocks not only the voltage-sensitive potassium conductance but also the conductance in the resting state, and that the latter may be possibly less sensitive to TEA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADRIAN R. H. THE RUBIDIUM AND POTASSIUM PERMEABILITY OF FROG MUSCLE MEMBRANE. J Physiol. 1964 Dec;175:134–159. doi: 10.1113/jphysiol.1964.sp007508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ARMSTRONG C. M., BINSTOCK L. ANOMALOUS RECTIFICATION IN THE SQUID GIANT AXON INJECTED WITH TETRAETHYLAMMONIUM CHLORIDE. J Gen Physiol. 1965 May;48:859–872. doi: 10.1085/jgp.48.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casteels R., Kitamura K., Kuriyama H., Suzuki H. The membrane properties of the smooth muscle cells of the rabbit main pulmonary artery. J Physiol. 1977 Sep;271(1):41–61. doi: 10.1113/jphysiol.1977.sp011989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Droogmans G., Raeymaekers L., Casteels R. Electro- and pharmacomechanical coupling in the smooth muscle cells of the rabbit ear artery. J Gen Physiol. 1977 Aug;70(2):129–148. doi: 10.1085/jgp.70.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fleckenstein A. Specific pharmacology of calcium in myocardium, cardiac pacemakers, and vascular smooth muscle. Annu Rev Pharmacol Toxicol. 1977;17:149–166. doi: 10.1146/annurev.pa.17.040177.001053. [DOI] [PubMed] [Google Scholar]
  6. Haeusler G., Thorens S. Effects of tetraethylammonium chloride on contractile, membrane and cable properties of rabbit artery muscle. J Physiol. 1980 Jun;303:203–224. doi: 10.1113/jphysiol.1980.sp013281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harder D. R., Sperelakis N. Action potentials induced in guinea pig arterial smooth muscle by tetraethylammonium. Am J Physiol. 1979 Jul;237(1):C75–C80. doi: 10.1152/ajpcell.1979.237.1.C75. [DOI] [PubMed] [Google Scholar]
  8. Henquin J. C. Tetraethylammonium potentiation of insulin release and inhibition of rubidium efflux in pancreatic islets. Biochem Biophys Res Commun. 1977 Jul 25;77(2):551–556. doi: 10.1016/s0006-291x(77)80014-4. [DOI] [PubMed] [Google Scholar]
  9. Hille B. The selective inhibition of delayed potassium currents in nerve by tetraethylammonium ion. J Gen Physiol. 1967 May;50(5):1287–1302. doi: 10.1085/jgp.50.5.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inomata H., Kao C. Y. Ionic currents in the guinea-pig taenia coli. J Physiol. 1976 Feb;255(2):347–378. doi: 10.1113/jphysiol.1976.sp011284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ito Y., Kuriyama H., Sakamoto Y. Effects of tetraethylammonium chloride on the membrane activity of guinea-pig stomach smooth muscle. J Physiol. 1970 Dec;211(2):445–460. doi: 10.1113/jphysiol.1970.sp009286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kirkpatrick C. T. Excitation and contraction in bovine tracheal smooth muscle. J Physiol. 1975 Jan;244(2):263–281. doi: 10.1113/jphysiol.1975.sp010796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kroeger E. A., Stephens N. L. Effect of tetraethylammonium on tonic airway smooth muscle: initiation of phasic electrical activity. Am J Physiol. 1975 Feb;228(2):633–636. doi: 10.1152/ajplegacy.1975.228.2.633. [DOI] [PubMed] [Google Scholar]
  14. Mironneau J., Savineau J. P. Effects of calcium ions on outward membrane currents in rat uterine smooth muscle. J Physiol. 1980 May;302:411–425. doi: 10.1113/jphysiol.1980.sp013253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ochi R., Nishiye H. Effect of intracellular tetraethylammonium ion on action potential in the guinea-pig's myocardium. Pflugers Arch. 1974 May 6;348(4):305–316. doi: 10.1007/BF00589220. [DOI] [PubMed] [Google Scholar]
  16. Osa T., Kuriyama H. The membrane properties and decremental conduction of excitation in the fundus of the guinea-pig stomach. Jpn J Physiol. 1970 Dec 15;20(6):626–639. doi: 10.2170/jjphysiol.20.626. [DOI] [PubMed] [Google Scholar]
  17. PERSOFF D. A. A comparison of methods for measuring efflux of labelled potassium from contracting rabbit atria. J Physiol. 1960 Jul;152:354–366. doi: 10.1113/jphysiol.1960.sp006492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Parod R. J., Putney J. W., Jr An alpha-adrenergic receptor mechanism controlling potassium permeability in the rat lacrimal gland acinar cell. J Physiol. 1978 Aug;281:359–369. doi: 10.1113/jphysiol.1978.sp012427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parod R. J., Putney J. W., Jr The role of calcium in the receptor mediated control of potassium permeability in the rat lacrimal gland. J Physiol. 1978 Aug;281:371–381. doi: 10.1113/jphysiol.1978.sp012428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Putney J. W., Jr Biphasic modulation of potassium release in rat parotid gland by carbachol and phenylephrine. J Pharmacol Exp Ther. 1976 Aug;198(2):375–384. [PubMed] [Google Scholar]
  21. Putney J. W., Jr, Parod R. J., Marier S. H. Control by calcium of protein discharge and membrane permeability to potassium in the rat lacrimal gland. Life Sci. 1977 Jun 1;20(11):1905–1911. doi: 10.1016/0024-3205(77)90227-2. [DOI] [PubMed] [Google Scholar]
  22. Sjodin R. A. Some Cation Interactions in Muscle. J Gen Physiol. 1961 May 1;44(5):929–962. doi: 10.1085/jgp.44.5.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stanfield P. R. The differential effects of tetraethylammonium and zinc ions on the resting conductance of frog skeletal muscle. J Physiol. 1970 Jul;209(1):231–256. doi: 10.1113/jphysiol.1970.sp009164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stanfield P. R. The effect of the tetraethylammonium ion on the delayed currents of frog skeletal muscle. J Physiol. 1970 Jul;209(1):209–229. doi: 10.1113/jphysiol.1970.sp009163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Suzuki H., Morita K., Kuriyama H. Innervation and properties of the smooth muscle of the dog trachea. Jpn J Physiol. 1976;26(3):303–320. doi: 10.2170/jjphysiol.26.303. [DOI] [PubMed] [Google Scholar]
  26. Tomita T., Yamamoto T. Effects of removing the external potassium on the smooth muscle of guinea-pig taenia coli. J Physiol. 1971 Feb;212(3):851–868. doi: 10.1113/jphysiol.1971.sp009360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vierhaus J., Ulbricht W. Rate of action of tetraethylammonium ions on the duration of action potentials in single Ranvier nodes. Pflugers Arch. 1971;326(1):88–100. doi: 10.1007/BF00586796. [DOI] [PubMed] [Google Scholar]
  28. Watanabe M., Imaizumi Y., Kasuya Y. The characteristics of tetraethylammonium-induced rhythmic contractions in canine tracheal smooth muscle: effect of Na-pump inhibition. Can J Physiol Pharmacol. 1979 Oct;57(10):1148–1156. doi: 10.1139/y79-170. [DOI] [PubMed] [Google Scholar]

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