In the Fig. 3 of ‘Red blood cell membrane-camouflaged nanoparticles loaded with AIEgen and Poly(I:C) for enhanced tumoral photodynamic-immunotherapy’ (National Science Review, Volume 8, Issue 6, 2021, nwab039, https://doi.org/10.1093/nsr/nwab039), the image showing M@P-pretreated PBMCs co-cultured with B16-F10 was provided incorrectly (Fig. 3h). The corrected version of Fig. 3 is presented below.
Figure 3.
Poly(I:C) promotes tumor cell death and simultaneously activates anti-tumor immunity. (a, b) Viability of (a) B16-F10 cells and (b) RAW 264.7 cells treated with PBS, M (red blood cell membrane), A (P2-PPh3), P (Poly(I:C)), M@P (NP without P2-PPh3), M@A (NP without Poly(I:C)) and M@AP by CCK-8 kit. (c) The expression levels of PCNA, BAX, c-Caspase3, PARP and GAPDH in B16-F10 cells treated with M@AP and controls detected by western blot. (d, e) The mRNA levels of immune factors in (d) B16-F10 and (e) PBMCs treated with M@AP and controls detected by qRT-PCR. (f, g) The protein levels of immune factors in (f) B16-F10 and (g) PBMCs treated with M@AP and controls detected by ELISA. (h) Upper: EGFP-B16-F10 cells were pretreated with PBS, M, A, P, M@P, M@A or M@AP, and then co-cultured with untreated PBMCs for 18 h. Lower: PBMCs were pretreated with PBS, M, A, P, M@P, M@A or M@AP, and then co-cultured with untreated EGFP-B16-F10 for 18 h. Dead EGFP-B16-F10 cells were shown in orange (merged green EGFP and red PI signals). Scale bar: 100 μm. The data were reported as mean ± SD and analyzed by two-sided Student's t-test (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, n.s. not significant.
Contributor Information
Jun Dai, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032, China.
Meng Wu, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032, China.
Quan Wang, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
Siyang Ding, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.
Xiaoqi Dong, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
Liru Xue, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032, China.
Qingqing Zhu, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032, China.
Jian Zhou, College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.
Fan Xia, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
Shixuan Wang, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032, China.
Yuning Hong, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.