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. 1981 Dec;321:49–64. doi: 10.1113/jphysiol.1981.sp013971

Stromal lactate accumulation can account for corneal oedema osmotically following epithelial hypoxia in the rabbit.

S D Klyce
PMCID: PMC1249613  PMID: 7338822

Abstract

1. The mechanism underlying stromal oedema subsequent to epithelial hypoxia was investigated in isolated rabbit corneas. 2. Stromas swelled about 20 micrometer following a 1 hr period of tear side hypoxia in both whole corneal isolates and in preparations in which fluid movement across the endothelium was blocked with silicone oil. In the experiments using whole corneas, stromal thickness was independent of tear side oxygen tension as long as aqueous humour PO2 was greater than 40 mmHg. 3. Neither epithelial thickness nor epithelial electrical resistance, a measure of total ion permeability, was significantly affected by blocking respiration. 4. A 10 degrees C reduction in corneal temperature markedly reduced the rate of hypoxic swelling, suggesting the involvement of a metabolism-dependent hydrating process and implicating the stromal accumulation of a catabolyte. 5. When CN- was used to mimic the hypoxic effect in isolated whole corneas, the passive 36Cl unidirectional flux was unaffected, but lactate production rate and stromal [lactate] more than doubled. 6. These measurements were used with a mathematical model for corneal hydration dynamics to examine the causes of hypoxic oedema. The principal conclusions were: epithelial hypoxia enhances epithelial lactate production and release to the stroma; this process causes an increase in stromal lactate concentration and a decrease in stromal NaCl concentration (primarily through dilution); stromal lactate accumulation exceeds in osmotic load and dilutional effect on [NaCl], producing stromal oedema. Whereas hypoxia produces corneal metabolic acidosis, effects on endothelial permeability of HCO3- transport need not be postulated to explain the stromal oedema that results from hypoxia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barr R. E., Silver I. A. Effects of corneal environment on oxygen tension in the anterior chambers of rabbits. Invest Ophthalmol. 1973 Feb;12(2):140–144. [PubMed] [Google Scholar]
  2. Dohlman C. H. The function of the corneal epithelium in health and disease. The Jonas S. Friedenwald Memorial Lecture. Invest Ophthalmol. 1971 Jun;10(6):383–407. [PubMed] [Google Scholar]
  3. Fatt I. Steady-state distribution of oxygen and carbon dioxide in the in vivo cornea. II. The open eye in nitrogen and the covered eye. Exp Eye Res. 1968 Jul;7(3):413–430. doi: 10.1016/s0014-4835(68)80056-9. [DOI] [PubMed] [Google Scholar]
  4. Gamm E. Das Säure-Basen-Gleichgewicht des Kammerwassers in Abhängigkeit von Kohlendioxid- und Sauerstoff-Diffusion durch die Cornea. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;214(2):101–105. doi: 10.1007/BF00572788. [DOI] [PubMed] [Google Scholar]
  5. HEDBYS B. O., DOHLMAN C. H. A new method for the determination of the swelling pressure of the corneal stroma in vitro. Exp Eye Res. 1963 Apr;2:122–129. doi: 10.1016/s0014-4835(63)80003-2. [DOI] [PubMed] [Google Scholar]
  6. Hedbys B. O., Mishima S. The thickness-hydration relationship of the cornea. Exp Eye Res. 1966 Jul;5(3):221–228. doi: 10.1016/s0014-4835(66)80010-6. [DOI] [PubMed] [Google Scholar]
  7. Hodson S., Miller F. The bicarbonate ion pump in the endothelium which regulates the hydration of rabbit cornea. J Physiol. 1976 Dec;263(3):563–577. doi: 10.1113/jphysiol.1976.sp011645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klyce S. D. Electrical profiles in the corneal epithelium. J Physiol. 1972 Oct;226(2):407–429. doi: 10.1113/jphysiol.1972.sp009991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klyce S. D. Enhancing fluid secretion by the corneal epithelium. Invest Ophthalmol Vis Sci. 1977 Oct;16(10):968–973. [PubMed] [Google Scholar]
  10. Klyce S. D., Maurice D. M. Automatic recording of corneal thickness in vitro. Invest Ophthalmol. 1976 Jul;15(7):550–553. [PubMed] [Google Scholar]
  11. Klyce S. D., Neufeld A. H., Zadunaisky J. A. The activation of chloride transport by epinephrine and Db cyclic-AMP in the cornea of the rabbit. Invest Ophthalmol. 1973 Feb;12(2):127–139. [PubMed] [Google Scholar]
  12. Klyce S. D., Russell S. R. Numerical solution of coupled transport equations applied to corneal hydration dynamics. J Physiol. 1979 Jul;292:107–134. doi: 10.1113/jphysiol.1979.sp012841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klyce S. D., Russell S. R. System for monitoring the thickness of transparent layered structures. Rev Sci Instrum. 1978 Sep;49(9):1318–1321. doi: 10.1063/1.1135575. [DOI] [PubMed] [Google Scholar]
  14. Klyce S. D. Transport of Na, Cl, and water by the rabbit corneal epithelium at resting potential. Am J Physiol. 1975 May;228(5):1446–1452. doi: 10.1152/ajplegacy.1975.228.5.1446. [DOI] [PubMed] [Google Scholar]
  15. LANGHAM M. E. Glycolysis in the cornea of the rabbit. J Physiol. 1954 Nov 29;126(2):396–403. doi: 10.1113/jphysiol.1954.sp005217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lambert S. R., Klyce S. D. The origins of Sattler's veil. Am J Ophthalmol. 1981 Jan;91(1):51–56. doi: 10.1016/0002-9394(81)90348-2. [DOI] [PubMed] [Google Scholar]
  17. Maurice D. M. Cellular membrane activity in the corneal endothelium of the intact eye. Experientia. 1968 Nov 15;24(11):1094–1095. doi: 10.1007/BF02147776. [DOI] [PubMed] [Google Scholar]
  18. Polse K. A., Mandell R. B. Critical oxygen tension at the corneal surface. Arch Ophthalmol. 1970 Oct;84(4):505–508. doi: 10.1001/archopht.1970.00990040507021. [DOI] [PubMed] [Google Scholar]
  19. Polse K. A., Mandell R. B. Hyperbaric oxygen effect on corneal edema caused by a contact lens. Am J Optom Arch Am Acad Optom. 1971 Mar;48(3):197–200. doi: 10.1097/00006324-197103000-00001. [DOI] [PubMed] [Google Scholar]
  20. Riley M. V. Aerobic glycolysis in the ox cornea. Exp Eye Res. 1969 Apr;8(2):201–204. doi: 10.1016/s0014-4835(69)80032-1. [DOI] [PubMed] [Google Scholar]
  21. Riley M. V. Glucose and oxygen utilization by the rabbit cornea. Exp Eye Res. 1969 Apr;8(2):193–200. doi: 10.1016/s0014-4835(69)80031-x. [DOI] [PubMed] [Google Scholar]
  22. SMELSER G. K., CHEN D. K. Physiological changes in cornea induced by contact lenses. AMA Arch Ophthalmol. 1955 May;53(5):676–679. doi: 10.1001/archopht.1955.00930010684009. [DOI] [PubMed] [Google Scholar]
  23. Thoft R. A., Friend J. Biochmical aspects of contact lens wear. Am J Ophthalmol. 1975 Jul;80(1):139–145. doi: 10.1016/0002-9394(75)90884-3. [DOI] [PubMed] [Google Scholar]
  24. Uniacke C. A., Augsburger A., Hill R. M. Epithelial swelling with oxygen insufficiency. Am J Optom Arch Am Acad Optom. 1971 Jul;48(7):565–568. doi: 10.1097/00006324-197107000-00004. [DOI] [PubMed] [Google Scholar]
  25. Weissman B. A., Fatt I., Rasson J. Diffusion of oxygen in human corneas in vivo. Invest Ophthalmol Vis Sci. 1981 Jan;20(1):123–125. [PubMed] [Google Scholar]
  26. Wilson G. S., Fatt I., Freeman R. D. Thickness changes in the stroma of an excised cornea during anoxia. Exp Eye Res. 1973 Oct 24;17(2):165–171. doi: 10.1016/0014-4835(73)90206-6. [DOI] [PubMed] [Google Scholar]
  27. Zantos S. G., Holden B. A. Transient endothelial changes soon after wearing soft contact lenses. Am J Optom Physiol Opt. 1977 Dec;54(12):856–858. doi: 10.1097/00006324-197712000-00010. [DOI] [PubMed] [Google Scholar]

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