Abstract
The stability of plasma glutathione peroxidase under different temperatures (4 degrees C vs. -15 degrees C), various durations of storage (0, 1, 2, 3, 7, 14, 28 and 56 d), and storage under inert gas (nitrogen (N2)) vs air is described. The glutathione peroxidase activity of swine plasma decreased consistently with storage at either 4 degrees C or -15 degrees C 1-56 d after collection, and differed (P less than or equal to 0.01) from the initial values. Storage under N2 at -15 degrees C slowed the rate of enzyme activity decrease but did not maintain the initial activity. For absolute measurements, it is suggested that swine plasma glutathione peroxidase activity be measured immediately after separation from the blood cells or be assayed within 24 h in plasma samples stored at -15 degrees C with air space displaced by N2. If relative treatment differences in enzyme activity are satisfactory, then assays can be conducted after controlled periods of storage.
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Selected References
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- Chow C. K., Tappel A. L. Response of glutathione peroxidase to dietary selenium in rats. J Nutr. 1974 Apr;104(4):444–451. doi: 10.1093/jn/104.4.444. [DOI] [PubMed] [Google Scholar]
- Koller L. D., South P. J., Exon J. H., Whitbeck G. A., Maas J. Comparison of selenium levels and glutathione peroxidase activity in bovine whole blood. Can J Comp Med. 1984 Oct;48(4):431–433. [PMC free article] [PubMed] [Google Scholar]
- Lawrence R. A., Sunde R. A., Schwartz G. L., Hoekstra W. G. Glutathione peroxidase activity in rat lens and other tissues in relation to dietary selenium intake. Exp Eye Res. 1974 Jun;18(6):563–569. doi: 10.1016/0014-4835(74)90062-1. [DOI] [PubMed] [Google Scholar]
- Levander O. A., DeLoach D. P., Morris V. C., Moser P. B. Platelet glutathione peroxidase activity as an index of selenium status in rats. J Nutr. 1983 Jan;113(1):55–63. doi: 10.1093/jn/113.1.55. [DOI] [PubMed] [Google Scholar]
- McGOVERN J. J., JONES A. R., STEINBERG A. G. The hematocrit of capillary blood. N Engl J Med. 1955 Aug 25;253(8):308–312. doi: 10.1056/NEJM195508252530802. [DOI] [PubMed] [Google Scholar]
- Paglia D. E., Valentine W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967 Jul;70(1):158–169. [PubMed] [Google Scholar]
- Rotruck J. T., Pope A. L., Ganther H. E., Swanson A. B., Hafeman D. G., Hoekstra W. G. Selenium: biochemical role as a component of glutathione peroxidase. Science. 1973 Feb 9;179(4073):588–590. doi: 10.1126/science.179.4073.588. [DOI] [PubMed] [Google Scholar]
- Scholz R. W., Hutchinson L. J. Distribution of glutathione peroxidase activity and selenium in the blood of dairy cows. Am J Vet Res. 1979 Feb;40(2):245–249. [PubMed] [Google Scholar]
- Sheppard A. D., Millar K. R. Stability of glutathione peroxidase in ovine blood samples under various storage conditions and the response of this enzyme to different methods of selenium supplementation. N Z Vet J. 1981 May;29(5):77–80. doi: 10.1080/00480169.1981.34805. [DOI] [PubMed] [Google Scholar]
- Smith P. J., Tappel A. L., Chow C. K. Glutathione peroxidase activity as a function of dietary selenomethionine. Nature. 1974 Feb 8;247(5440):392–393. doi: 10.1038/247392a0. [DOI] [PubMed] [Google Scholar]
- Thompson K. G., Fraser A. J., Harrop B. M., Kirk J. A. Glutathione peroxidase activity in bovine serum and erythrocytes in relation to selenium concentrations of blood, serum and liver. Res Vet Sci. 1980 May;28(3):321–324. [PubMed] [Google Scholar]