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. 1987 Jan;51(1):126–134.

Changes in N-acetyl-B-D-glucosaminidase and B-glucuronidase activities in milk during bovine mastitis.

H Nagahata, S Saito, H Noda
PMCID: PMC1255286  PMID: 3567747

Abstract

To determine the N-acetyl-B-D-glucosaminidase (NAGase) and B-glucuronidase (B-Gase) activities in mastitic milk, basic enzyme assay conditions, distribution of NAGase and B-Gase, comparison of their activities with California Mastitis Test scores, and the effects of the milking process on their enzyme activities were examined. The mean NAGase and B-Gase activities in milk macrophages were about threefold higher than those of milk and blood polymorphonuclear cells. Very little NAGase activity appeared to be associated with blood mononuclear cells, whereas a relatively higher B-Gase activity was observed. California Mastitis Test scores of each group (1 to 5) appeared to be well correlated (r = 0.86 for NAGase and 0.92 for B-Gase) with the levels of NAGase and B-Gase activity. The milking process was least effective in the normal milk, but some variations of enzyme activities during milking in mastitic milk were found. Changes in NAGase and B-Gase activities in quarter milk were well monitored during the course of clinical mastitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bakken G., Thorburn M. Seriousness and stability of subclinical mastitis assessed by quarter milk serum albumin. Acta Vet Scand. 1985;26(2):273–285. doi: 10.1186/BF03546557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fleet I. R., Linzell J. L., Peaker M. The use of an autoanalyzer for the rapid analysis of milk constituents affected by subclinical mastitis. Br Vet J. 1972 Jun;128(6):297–300. doi: 10.1016/s0007-1935(17)36934-8. [DOI] [PubMed] [Google Scholar]
  3. Healy P. J. Lysosomal hydrolase activity in leucocytes from cattle, sheep, goats, horses and pigs. Res Vet Sci. 1982 Nov;33(3):275–279. [PubMed] [Google Scholar]
  4. Kiermeier F., Güll J. Uber das Vorkommen der beta-Glucuronidase in Kuhmilch. Naturwissenschaften. 1966 Dec;53(23):613–614. doi: 10.1007/BF00632280. [DOI] [PubMed] [Google Scholar]
  5. Kitchen B. J. Enzymic methods for estimation of the somatic cell count in bovine milk. 1. Development of assay techniques and a study of their usefulness in evaluating the somatic cell content of milk. J Dairy Res. 1976 Jun;43(2):251–258. doi: 10.1017/s0022029900015806. [DOI] [PubMed] [Google Scholar]
  6. Kitchen B. J., Kwee W. S., Middleton G., Andrews R. J. Relationship between the level of N-acetyl-beta-D-glucosaminidase (NAGase) in bovine milk and the presence of mastitis pathogens. J Dairy Res. 1984 Feb;51(1):11–16. doi: 10.1017/s0022029900023268. [DOI] [PubMed] [Google Scholar]
  7. Kitchen B. J., Middleton G. Enzymic methods for the estimation of the somatic cell count in bovine milk. II. N-Acetyl-beta-D-glucosaminidase test for routine estimation of the somatic cell count in milk. J Dairy Res. 1976 Oct;43(3):491–494. doi: 10.1017/s0022029900016071. [DOI] [PubMed] [Google Scholar]
  8. Kitchen B. J., Middleton G., Salmon M. Bovine milk N-acetyl-beta-D-glucosaminidase and its significance in the detection of abnormal udder secretions. J Dairy Res. 1978 Feb;45(1):15–20. doi: 10.1017/s0022029900016149. [DOI] [PubMed] [Google Scholar]
  9. Mellors A. Beta-N-acetylglucosaminase in bovine milk. Can J Biochem. 1968 May;46(5):451–455. doi: 10.1139/o68-068. [DOI] [PubMed] [Google Scholar]
  10. Nagahata H., Noda H., Kudo T. Optimum density of the separating fluid for centrifugal separation of lymphocytes from bovine peripheral blood. Nihon Juigaku Zasshi. 1982 Dec;44(6):957–960. doi: 10.1292/jvms1939.44.957. [DOI] [PubMed] [Google Scholar]
  11. Obara Y., Komatsu M. Relationship between N-acetyl-beta-D-glucosaminidase activity and cell count, lactose, chloride, or lactoferrin in cow milk. J Dairy Sci. 1984 May;67(5):1043–1046. doi: 10.3168/jds.S0022-0302(84)81404-6. [DOI] [PubMed] [Google Scholar]
  12. Obara Y., Nakano T., Kume T., Ohshima M. Mutual relationship between milk components and lysosome enzymatic activity in abnormal milk. Nihon Juigaku Zasshi. 1983 Apr;45(2):203–208. doi: 10.1292/jvms1939.45.203. [DOI] [PubMed] [Google Scholar]
  13. Sandholm M., Honkanen-Buzalski T., Kangasniemi R. Milk trypsin-inhibitor capacity as an indicator of bovine mastitis--a novel principle which can be automated. J Dairy Res. 1984 Feb;51(1):1–9. doi: 10.1017/s0022029900023256. [DOI] [PubMed] [Google Scholar]

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