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. 1987 Apr;51(2):157–161.

Serum susceptibility of bovine pasteurellas.

K A Blau, A C Ward, D J Prieur, L B Corbeil
PMCID: PMC1255295  PMID: 3300919

Abstract

In this study, the serum sensitivity of 23 P. haemolytica isolates and 18 P. multocida isolates was determined by incubating dilutions of bacteria with equal volumes of fresh or heat-inactivated bovine serum for one, two, or three hours. Clinical isolates of both Pasteurella species were resistant to serum, whereas isolates from asymptomatic cattle varied in serum susceptibility. The classical pathway of complement appeared to be the principal means of complement mediated killing as detected by incubation in the presence or absence of EGTA-MgCl2. Lyzozyme and iron saturation of serum did not greatly affect serum susceptibility with either of the Pasteurella species.

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Selected References

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  1. Binns M. M., Mayden J., Levine R. P. Further characterization of complement resistance conferred on Escherichia coli by the plasmid genes traT of R100 and iss of ColV,I-K94. Infect Immun. 1982 Feb;35(2):654–659. doi: 10.1128/iai.35.2.654-659.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carter G. R. Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Adv Vet Sci. 1967;11:321–379. [PubMed] [Google Scholar]
  3. Corbeil L. B., Blau K., Prieur D. J., Ward A. C. Serum susceptibility of Haemophilus somnus from bovine clinical cases and carriers. J Clin Microbiol. 1985 Aug;22(2):192–198. doi: 10.1128/jcm.22.2.192-198.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Corbeil L. B., Wunderlich A. C., Ito J. I., McCutchan J. A. Plaque assay for measuring serum bactericidal activity against gonococci. J Clin Microbiol. 1978 Nov;8(5):618–620. doi: 10.1128/jcm.8.5.618-620.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Durack D. T., Beeson P. B. Protective role of complement in experimental Escherichia coli endocarditis. Infect Immun. 1977 Apr;16(1):213–217. doi: 10.1128/iai.16.1.213-217.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eisenstein B. I., Lee T. J., Sparling P. F. Penicillin sensitivity and serum resistance are independent attributes of strains of Neisseria gonorrhoeae causing disseminated gonococcal infection. Infect Immun. 1977 Mar;15(3):834–841. doi: 10.1128/iai.15.3.834-841.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frank G. H., Smith P. C. Prevalence of Pasteurella haemolytica in transported calves. Am J Vet Res. 1983 Jun;44(6):981–985. [PubMed] [Google Scholar]
  8. Frank G. H., Wessman G. E. Rapid plate agglutination procedure for serotyping Pasteurella haemolytica. J Clin Microbiol. 1978 Feb;7(2):142–145. doi: 10.1128/jcm.7.2.142-145.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jensen R., Pierson R. E., Braddy P. M., Saari D. A., Lauerman L. H., England J. J., Keyvanfar H., Collier J. R., Horton D. P., McChesney A. E. Shipping fever pneumonia in yearling feedlot cattle. J Am Vet Med Assoc. 1976 Sep 1;169(5):500–506. [PubMed] [Google Scholar]
  10. MacDonald J. T., Maheswaran S. K., Opuda-Asibo J., Townsend E. L., Thies E. S. Susceptibility of Pasteurella haemolytica to the bactericidal effects of serum, nasal secretions and bronchoalveolar washings from cattle. Vet Microbiol. 1983 Nov;8(6):585–599. doi: 10.1016/0378-1135(83)90007-x. [DOI] [PubMed] [Google Scholar]
  11. McCutchan J. A., Levine S., Braude A. I. Influence of colony type on susceptibility of gonococci to killing by human serum. J Immunol. 1976 Jun;116(6):1652–1655. [PubMed] [Google Scholar]
  12. Osserman E. F., Lawlor D. P. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966 Nov 1;124(5):921–952. doi: 10.1084/jem.124.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Payne S. M., Holmes K. K., Finkelstein R. A. Role of iron in disseminated gonococcal infections. Infect Immun. 1978 May;20(2):573–574. doi: 10.1128/iai.20.2.573-574.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Prieur D. J., Cámara V. M. Inheritance of lysozyme deficiency in rabbits. J Hered. 1979 May-Jun;70(3):181–184. doi: 10.1093/oxfordjournals.jhered.a109230. [DOI] [PubMed] [Google Scholar]
  15. Prieur D. J., Olson H. M., Young D. M. Lysozyme deficiency-an inherited disorder of rabbits. Am J Pathol. 1974 Nov;77(2):283–298. [PMC free article] [PubMed] [Google Scholar]
  16. Rice P. A., McCormack W. M., Kasper D. L. Natural serum bactericidal activity against Neisseria gonorrhoeae isolates from disseminated, locally invasive, and uncomplicated disease. J Immunol. 1980 May;124(5):2105–2109. [PubMed] [Google Scholar]
  17. Rimler R. B., Brogden K. A. Pasteurella multocida isolated from rabbits and swine: serologic types and toxin production. Am J Vet Res. 1986 Apr;47(4):730–737. [PubMed] [Google Scholar]
  18. Schoolnik G. K., Buchanan T. M., Holmes K. K. Gonococci causing disseminated gonococcal infection are resistant to the bactericidal action of normal human sera. J Clin Invest. 1976 Nov;58(5):1163–1173. doi: 10.1172/JCI108569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shafer W. M., Guymon L. F., Sparling P. F. Identification of a new genetic site (sac-3+) in Neisseria gonorrhoeae that affects sensitivity to normal human serum. Infect Immun. 1982 Mar;35(3):764–769. doi: 10.1128/iai.35.3.764-769.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sutton A., Schneerson R., Kendall-Morris S., Robbins J. B. Differential complement resistance mediates virulence of Haemophilus influenzae type b. Infect Immun. 1982 Jan;35(1):95–104. doi: 10.1128/iai.35.1.95-104.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor P. W. Bactericidal and bacteriolytic activity of serum against gram-negative bacteria. Microbiol Rev. 1983 Mar;47(1):46–83. doi: 10.1128/mr.47.1.46-83.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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