Abstract
Sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60Co source. Surviving test organisms were quantitatively determined by selective and enrichment techniques. The experiment was modeled as a quantal assay in which probit analysis was applied to obtain D10 values. The D10 value represents the irradiating dose required to reduce the population by 90%. The D10 value ranged from 13.4 krad for Campylobacter fetus to 156.6 krad for Streptococcus faecalis in animal sewage. However, the D10 value for the laboratory effluent was generally lower. Based on the estimated D10 values, the rating of the test organisms in decreasing order of radiosensitivity appeared as follows: Brucella abortus, Campylobacter fetus subsp. fetus, Campylobacter jejuni, Campylobacter coli, Campylobacter laridis, Mycobacterium fortuitum, Aspergillus fumigatus, Salmonella muenster, Candida albicans, Clostridium difficile and Streptococcus faecalis. If the D5 and D1 values were utilized, this listing would be only slightly altered.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brunborg G., Resnick M. A., Williamson D. H. Cell-cycle-specific repair of DNA double strand breaks in Saccharomyces cerevisiae. Radiat Res. 1980 Jun;82(3):547–558. [PubMed] [Google Scholar]
- Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol. 1979 Sep;8(3):737–765. [PubMed] [Google Scholar]
- Dudley D. J., Guentzel M. N., Ibarra M. J., Moore B. E., Sagik B. P. Enumeration of potentially pathogenic bacteria from sewage sludges. Appl Environ Microbiol. 1980 Jan;39(1):118–126. doi: 10.1128/aem.39.1.118-126.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Etzel J. E., Born G. S., Stein J., Helbing T. J., Baney G. Sewage sludge conditioning and disinfection by gamma irradiation. Am J Public Health Nations Health. 1969 Nov;59(11):2067–2076. doi: 10.2105/ajph.59.11.2067. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garcia M. M., Lior H., Stewart R. B., Ruckerbauer G. M., Trudel J. R., Skljarevski A. Isolation, characterization, and serotyping of Campylobacter jejuni and Campylobacter coli from slaughter cattle. Appl Environ Microbiol. 1985 Mar;49(3):667–672. doi: 10.1128/aem.49.3.667-672.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosef O. Isolation of Campylobacter fetus subsp. jejuni from the gallbladder of normal slaughter pigs, using an enrichment procedure. Acta Vet Scand. 1981;22(1):149–151. doi: 10.1186/BF03547219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruckerbauer G. M., Malkin K., Mitchell D., Boulanger P. Vibriosis: demonstration of Vibrio fetus and Vibrio bubulus organisms in preputial fluid by immunofluorescence and cultural techniques. Can J Comp Med. 1974 Jul;38(3):321–327. [PMC free article] [PubMed] [Google Scholar]
- Thomas F. C., Ouwerkerk T., McKercher P. Inactivation by gamma irradiation of animal viruses in simulated laboratory effluent. Appl Environ Microbiol. 1982 May;43(5):1051–1056. doi: 10.1128/aem.43.5.1051-1056.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward R. L., Yeager J. G., Ashley C. S. Response of bacteria in wastewater sludge to moisture loss by evaporation and effect of moisture content on bacterial inactivation by ionizing radiation. Appl Environ Microbiol. 1981 May;41(5):1123–1127. doi: 10.1128/aem.41.5.1123-1127.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wizigmann I. Vergleichende bakteriologische Untersuchungen bei der Behandlung von Klärschlamm mit Gammastrahlen. Zentralbl Bakteriol B. 1978 Dec;167(5-6):546–562. [PubMed] [Google Scholar]