Abstract
The relative importance of various enteric viruses associated with diarrhea of turkey poults was investigated by an evaluation of specimens received since 1982. Specimens originated from one to eight week old turkey poults, with mild to severe diarrhea, from 114 flocks in 42 commercial operations located in southern Quebec. The acute phase of enteritis occurred usually in poults between two and four weeks of age. Clarified intestinal contents were examined by direct electron microscopy and enzyme immunoassays. Enzyme-linked immunosorbent assays were performed with antisera to bovine rotavirus group antigen, avian reovirus types 1 to 5, and the prototype strain of the turkey enteric coronavirus. The presence of viruses could be demonstrated by electron microscopy in 55.3% of the specimens, and at least five different viruses were incriminated either alone or in combination. The coronavirus was by far the most common enteric virus with a prevalence of 47.5%. By enzyme-linked immunosorbent assay, rotavirus, reovirus and turkey coronavirus were detected in 14.5%, 18.1% and 61.4% of the specimens, respectively. By electron microscopy, 56.6% of these cases were positive for at least one virus.
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- Archambault D., Roy R. S., Dea S., Elazhary M. A. Comparative study of bovine rotavirus isolates by plaque assay. Can J Comp Med. 1984 Jul;48(3):286–289. [PMC free article] [PubMed] [Google Scholar]
- Bellamy K., Gardner P. S., Hambling M. H., Rice S., Bradburne A. F. Enzyme-linked immunosorbent assay for the detection of human rotavirus in stools. J Virol Methods. 1983 Aug;7(2):65–72. doi: 10.1016/0166-0934(83)90093-9. [DOI] [PubMed] [Google Scholar]
- Dea S., Marsolais G., Beaubien J., Ruppanner R. Coronaviruses associated with outbreaks of transmissible enteritis of turkeys in Quebec: hemagglutination properties and cell cultivation. Avian Dis. 1986 Apr-Jun;30(2):319–326. [PubMed] [Google Scholar]
- Dea S., Roy R. S., Begin M. E. Counterimmunoelectroosmophoresis for detection of neonatal calf diarrhea coronavirus: methodology and comparison with electron microscopy. J Clin Microbiol. 1979 Aug;10(2):240–244. doi: 10.1128/jcm.10.2.240-244.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dea S., Roy R. S., Elazhary M. A. Virus-like particles, 45 to 65 nm, in intestinal contents of neonatal calves. Can J Comp Med. 1983 Jan;47(1):88–91. [PMC free article] [PubMed] [Google Scholar]
- Deshmukh D. R., Larsen C. T., Pomeroy B. S. Survival of bluecomb agent in embryonating turkey eggs and cell cultures. Am J Vet Res. 1973 May;34(5):673–675. [PubMed] [Google Scholar]
- Deshmukh D. R., Pomeroy B. S. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 1969 May;13(2):239–243. [PubMed] [Google Scholar]
- King D. J. Comments on the etiology and immunity of transmissible (coronaviral) enteritis of turkeys (bluecomb). Am J Vet Res. 1975 Apr;36(4 Pt 2):555–556. [PubMed] [Google Scholar]
- Lapierre J., Marsolais G., Pilon P., Descôteaux J. P. Preliminary report on the observation of a coronavirus in the intestine of the laboratory rabbit. Can J Microbiol. 1980 Oct;26(10):1204–1208. doi: 10.1139/m80-201. [DOI] [PubMed] [Google Scholar]
- McNulty M. S., Allan G. M., Todd D., McFerran J. B. Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Arch Virol. 1979;61(1-2):13–21. doi: 10.1007/BF01320587. [DOI] [PubMed] [Google Scholar]
- McNulty M. S., Curran W. L., McFerran J. B. Detection of astroviruses in turkey faeces by direct electron microscopy. Vet Rec. 1980 Jun 28;106(26):561–561. doi: 10.1136/vr.106.26.561. [DOI] [PubMed] [Google Scholar]
- Nersessian B. N., Goodwin M. A., Kleven S. H., Pesti D. Studies on orthoreoviruses isolated from young turkeys. I. Isolation and characterization. Avian Dis. 1985 Jul-Sep;29(3):755–767. [PubMed] [Google Scholar]
- Nersessian B. N., Goodwin M. A., Page R. K., Kleven S. H. Studies on orthoreoviruses isolated from young turkeys. II. Virus distribution in organs and serological response of poults inoculated orally. Avian Dis. 1985 Oct-Dec;29(4):963–969. [PubMed] [Google Scholar]
- Panigrahy B., Naqi S. A., Hall C. F. Isolation and characterization of viruses associated with transmissible enteritis (bluecomb) of turkeys. Avian Dis. 1973 Apr-Jun;17(2):430–438. [PubMed] [Google Scholar]
- Pomeroy K. A., Patel B. L., Larsen C. T., Pomeroy B. S. Combined immunofluorescence and transmission electron microscopic studies of sequential intestinal samples from turkey embryos and poults infected with turkey enteritis coronavirus. Am J Vet Res. 1978 Aug;39(8):1348–1354. [PubMed] [Google Scholar]
- Saif L. J., Saif Y. M., Theil K. W. Enteric viruses in diarrheic turkey poults. Avian Dis. 1985 Jul-Sep;29(3):798–811. [PubMed] [Google Scholar]
- Spence L., Fauvel M., Petro R., Babiuk L. A. Comparison of rotavirus strains by hemagglutination inhibition. Can J Microbiol. 1978 Apr;24(4):353–362. doi: 10.1139/m78-059. [DOI] [PubMed] [Google Scholar]
- Trampel D. W., Kinden D. A., Solorzano R. F., Stogsdill P. L. Parvovirus-like enteropathy in Missouri turkeys. Avian Dis. 1983 Jan-Mar;27(1):49–54. [PubMed] [Google Scholar]
- Wyeth J. P., Chettle N. J., Labram J. Avian calicivirus. Vet Rec. 1981 Nov 21;109(21):477–477. doi: 10.1136/vr.109.21.477-b. [DOI] [PubMed] [Google Scholar]
- Yason C. V., Schat K. A. Isolation and characterization of avian rotaviruses. Avian Dis. 1985 Apr-Jun;29(2):499–508. [PubMed] [Google Scholar]
- Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]