Abstract
Enzyme-linked immunosorbent assay was used to detect serum antibodies to mouse thymic virus, a herpesvirus that causes thymic lesions and immunosuppression. Antibodies were detected in mice that had received single or multiple injections of the virus and were also found in mice housed in contact with the experimentally infected animals. By contrast, mice not exposed to mouse thymic virus or those inoculated with an uninfected thymus preparation remained seronegative. A serological survey of eight mouse colonies revealed one positive colony, confirmed by virus isolation. These results show that the test is sufficiently sensitive and specific to be used for routine screening of mice.
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Selected References
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- Cohen P. L., Cross S. S., Mosier D. E. Immunologic effects of neonatal infection with mouse thymic virus. J Immunol. 1975 Sep;115(3):706–710. [PubMed] [Google Scholar]
- Collins M. J., Jr, Parker J. C. Murine virus contaminants of leukemia viruses and transplantable tumors. J Natl Cancer Inst. 1972 Oct;49(4):1139–1143. [PubMed] [Google Scholar]
- Cross S. S., Morse H. C., Asofsky R. Neonatal infection with mouse thymic virus. Differential effects on T cells mediating the graft-versus-host reaction. J Immunol. 1976 Aug;117(2):635–638. [PubMed] [Google Scholar]
- Cross S. S., Parker J. C., Rowe W. P., Robbins M. L. Biology of mouse thymic virus, a herpesvirus of mice, and the antigenic relationship to mouse cytomegalovirus. Infect Immun. 1979 Dec;26(3):1186–1195. doi: 10.1128/iai.26.3.1186-1195.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lussier G., Guénette D., Descôteaux J. P. Comparison of serological tests for the detection of antibody to natural and experimental murine cytomegalovirus. Can J Vet Res. 1987 Apr;51(2):249–252. [PMC free article] [PubMed] [Google Scholar]
- ROWE W. P., CAPPS W. I. A new mouse virus causing necrosis of the thymus in newborn mice. J Exp Med. 1961 May 1;113:831–844. doi: 10.1084/jem.113.5.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
- St-Pierre Y., Potworowski E. F., Lussier G. Transmission of mouse thymic virus. J Gen Virol. 1987 Apr;68(Pt 4):1173–1176. doi: 10.1099/0022-1317-68-4-1173. [DOI] [PubMed] [Google Scholar]
- Wood B. A., Dutz W., Cross S. S. Neonatal infection with mouse thymic virus: spleen and lymph node necrosis. J Gen Virol. 1981 Nov;57(Pt 1):139–147. doi: 10.1099/0022-1317-57-1-139. [DOI] [PubMed] [Google Scholar]