There was an error in the original publication [1]. In the Materials and Methods Section, the sequence of the Pan-CoV_F-1 primer is missing a T, but it is shown correctly in the Supplementary Material.
A correction has been made to 2. Materials and Methods, 2.1 Primer Design and Optimization:
2.1. Primer Design and Optimization
We obtained the sequence of the previously published Watanabe et al. [23] primers 5′-GGTTGGGACTATCCTAAGTGTGA-3′ (Watanabe conventional_F) and 5′-CCATCATCAGATAGAATCATCATA-3′ (Watanabe conventional_R). These primers were mapped to 48 CoVs, which represented all four genera, allowing identification of mismatches. We then redesigned these primers. The primer sequences of the first PCR are: 5′-GGTTGGGAYTAYCCHAARTGYGA-3′ (Pan_CoV_F-1), 5′-CCRTCATCAGAHARWATCAT-3′ (Pan_CoV_R-1), and 5′-CCRTCATCACTHARWATCAT-3′ (Pan_CoV_R-2). The semi-nested second PCR utilizes the same reverse primers and 5′-GAYTAYCCHAARTGTGAYAGA-3′ (Pan_CoV_F-2) and 5′-GAYTAYCCHAARTGTGAYMGH-3′ (Pan_CoV_F-3) as forward primers (Table S1).
The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Footnotes
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Reference
- 1.Holbrook M.G., Anthony S.J., Navarrete-Macias I., Bestebroer T., Munster V.J., van Doremalen N. Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera. Viruses. 2021;13:599. doi: 10.3390/v13040599. [DOI] [PMC free article] [PubMed] [Google Scholar]