Erratum

Erratum

Intracellular trafficking pathway of newly synthesized CD1b molecules

V.Briken, R.M.Jackman, S.Dasgupta, S.Hoening and S.A.Porcelli

The EMBO Journal, 21, 825–834, 2001

In the above paper, the names of two cytoplasmic tail peptides were incorrectly printed in Table I. The correct version should read:

Table I. Overview of cytoplasmic tail peptide interactions with AP-2 or AP-3.

Tail	AP-2			AP-3
	ka (1/M×s)	kd (1/s)	KD (µM)	ka (1/M×s)	kd (1/s)	KD (µM)
CD1c wt	1.9 × 104	5.6 × 10–3	0.29	3.4 × 102	4.7 × 10–3	14.0
CD1b wt	1.4 × 104	4.9 × 10–3	0.35	1.2 × 103	4.2 × 10–3	3.5
CD1b Y>A	1.2 × 103	5.3 × 10–3	4.4	0.3 × 103	4.0 × 10–3	13.3
Lamp-1 wt	2.6 × 104	3.1 × 10–3	0.12	9.2 × 102	3.4 × 10–3	3.7
Lamp-1 Y>A	8.7 × 103	3.9 × 10–3	4.5	2.7 × 102	3.9 × 10–3	14.4
TfR wt	1.2 × 105	4.6 × 10–3	0.04	and	nd	nd
TfR Y,F>A	2.3 × 103	5.2 × 10–3	2.3	nd	nd	nd

The interaction of purified AP-2 and AP-3 complexes with cytoplasmic tail peptides was analyzed by SPR using a BIAcore 3000 as described in Materials and methods. The rate constants for association (k_a) and dissociation (k_d) were determined at three different concentrations of AP-2 and AP-3 for each peptide. Shown are the values measured with AP-2 and AP-3 at 500 nM. The equilibrium dissociation constant (K_D = k_d/k_a) was calculated for each adaptor concentration. Values obtained from three independent sets of measurements varied by 3–7%.

^and: not detectable.

The publisher would like to apologize for this error and any confusion caused.