Correction for Mittal et al., Mechanisms underlying allosteric modulation of antiseizure medication binding to synaptic vesicle protein 2A (SV2A)

Correction for “Mechanisms underlying allosteric modulation of antiseizure medication binding to synaptic vesicle protein 2A (SV2A),” by Anshumali Mittal, Matthew F. Martin, Laurent Provins, Adrian Hall, Marie Ledecq, Christian Wolff, Michel Gillard, Peter S. Horanyi, and Jonathan A. Coleman, which published September 2, 2025; 10.1073/pnas.2510239122 (Proc. Natl. Acad. Sci. U.S.A. 122, e2510239122).

The authors note that, due to a production error, Fig. 1 appeared incorrectly. The authors note that “the updated Fig. 1 includes chemical drawings of padsevonil and UCB2500.” The corrected figure and its legend appear below. The online version has been corrected.

Fig. 1.

UCB1244283 exerts an allosteric effect on the SV2A-LEV complex. (A) Chemical structures of SV2A-specific ligands, Levetiracetam (LEV) [2S-(2-oxo-1-pyrrolidinyl) butanamide], Brivaracetam (BRV) [(2S)-2-((4R)-2-oxo-4-propylpyrrolidin-1-yl)butanamide], Padsevonil (PSL) [(4R)-4-(2-chloro-2,2-difluoroethyl)-1-[[2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl]pyrrolidin-2-one], UCB2500 [(4S)-1-[[2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl]-4-(4,4,4-trifluorobutyl)pyrrolidin-2-one], and UCB1244283 [4-(3,5-dimethylphenyl)-N-(2-methoxyphenyl)-3-methylbutanamide], chiral center is shown with red circle. (B) Thermostability of mVenus-tagged SV2AΔ64 with no ligand (red), 20 µM BRV (green), 30 µM UCB1244283 (black), BRV-UCB1244283 (blue), and LEV-UCB1244283 (cyan). 100 µM LEV was used for the LEV-UCB1244283 experiment. (C) UCB1244283 (30 µM) increases the maximum specific binding (B_max) of ³H-LEV (~4 µM) to SV2A. (D) Concentration–response curve for measuring EC₅₀ of UC1244283 (30 μM, 15 μM, 1 μM, 100 nM, 10 nM, and 1 nM) by binding of ³H-LEV (~4 μM) to SV2A. The EC₅₀ was calculated 0.7 ± 0.3 μM by nonlinear regression using a logarithmic concentration response sigmoidal curve. Data are shown as mean ± SEM (n = 7). (E) ³H-LEV saturation binding experiments for measuring LEV binding affinity to SV2A (Kd = 3.8 ± 0.8 μM) in presence of UCB1244283. Data are shown as mean ± SEM (n = 8).