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. 1991 Jul;60(1):45–52. doi: 10.1016/S0006-3495(91)82029-X

Vibrational structure of the formyl group on heme a. Implications on the properties of cytochrome c oxidase.

S W Han 1, Y C Ching 1, S L Hammes 1, D L Rousseau 1
PMCID: PMC1260037  PMID: 1653051

Abstract

Resonance Raman spectra have been recorded for heme a derivatives in which the oxygen atom of the formyl group has been isotopically labeled and for Schiff base derivatives of heme a in which the Schiff base nitrogen has been isotopically labeled. The 14N-15N isotope shift in the C = N stretching mode of the Schiff base is close to the theoretically predicted shift for an isolated C = N group for both the ferric and ferrous oxidation states and in both aqueous and nonaqueous solutions. In contrast, the 16O-18O isotope shift of the C = O stretching mode of the formyl group is significantly smaller than that predicted for an isolated C = O group and is also dependent on whether the environment is aqueous or nonaqueous. This differences between the theoretically predicted shifts and the observed shifts are attributed to coupling of the C = O stretching mode to as yet unidentified modes of the heme. The complex behavior of the C = O stretching vibration precludes the possibility of making simple interpretations of frequency shifts of this mode in cytochrome c oxidase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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