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. 1992 Mar;61(3):631–638. doi: 10.1016/S0006-3495(92)81868-4

The efficiency of malachite green, free and protein bound, as a photon-to-heat converter

Guilherme L Indig *, Daniel G Jay ‡, Joseph J Grabowski *
PMCID: PMC1260281  PMID: 19431819

Abstract

Dye assisted laser inactivation of proteins has been found to be a methodology that can achieve high selectivity. Despite the fact that the methodology is successful, knowledge of the detailed inactivation mechanism would allow full optimization of this technique. Here, pulsed-laser photoacoustic calorimetry is used to study the photophysical properties, principally the heat release behavior, of protein bound malachite green. We found that when bound to bovine serum albumin the dye is a good photon-to-heat converter, but ∼2.6% of the absorbed photon energy (λexc = 624 nm) is not released as heat in less than 10 μs. This observation suggests that a mechanism other than simple heat-induced inactivation may be the principle process; a long lived excited triplet state of malachite green (or species derived from it) is postulated to play a major role.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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