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. 1992 Apr;61(4):936–940. doi: 10.1016/S0006-3495(92)81900-8

Cryo-EM of the native structure of the calcium release channel/ryanodine receptor from sarcoplasmic reticulum.

M Radermacher 1, T Wagenknecht 1, R Grassucci 1, J Frank 1, M Inui 1, C Chadwick 1, S Fleischer 1
PMCID: PMC1260352  PMID: 1316182

Abstract

The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.

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Selected References

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