Abstract
In previous studies of middle ear muscles, the classification of fibre types by histochemical methods was particularly difficult in the bovine and porcine tensor tympani muscle, suggesting the presence of immature fibres. We therefore reexamined the tensor tympani from pigs and cattle of various ages immunohistochemically, using a panel of antimyosin antibodies, including one (anti-NE) specific for neonatal and embryonic myosins. Fibres positive to anti-NE were found in tensor tympani in both species in all ages examined; only a few of these fibres reacted exclusively with this antibody; some also contained slow myosin and the majority also contained adult fast (type IIA) myosin. Furthermore, although the remaining fibres included some of the classical types I and IIA, the majority of them showed a mismatch between their histochemical and immunohistochemical profiles. The morphological appearance of the muscle, the widespread presence of neonatal myosin (often together with another myosin in the same fibre) and the persistence of this composition from birth to adulthood, could be explained by an incomplete development of the muscle fibres, resulting in a 'muscle' much better suited to the role of a ligament.
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- Ashmore C. R., Vigneron P., Marger L., Doerr L. Simultaneous cytochemical demonstration of muscle fiber types and acetylcholinesterase in muscle fibers of dystrophic chickens. Exp Neurol. 1978 May 15;60(1):68–82. doi: 10.1016/0014-4886(78)90169-3. [DOI] [PubMed] [Google Scholar]
- Billeter R., Weber H., Lutz H., Howald H., Eppenberger H. M., Jenny E. Myosin types in human skeletal muscle fibers. Histochemistry. 1980;65(3):249–259. doi: 10.1007/BF00493174. [DOI] [PubMed] [Google Scholar]
- Butler-Browne G. S., Eriksson P. O., Laurent C., Thornell L. E. Adult human masseter muscle fibers express myosin isozymes characteristic of development. Muscle Nerve. 1988 Jun;11(6):610–620. doi: 10.1002/mus.880110614. [DOI] [PubMed] [Google Scholar]
- Gambke B., Rubinstein N. A. A monoclonal antibody to the embryonic myosin heavy chain of rat skeletal muscle. J Biol Chem. 1984 Oct 10;259(19):12092–12100. [PubMed] [Google Scholar]
- Jacoby J., Ko K., Weiss C., Rushbrook J. I. Systematic variation in myosin expression along extraocular muscle fibres of the adult rat. J Muscle Res Cell Motil. 1990 Feb;11(1):25–40. doi: 10.1007/BF01833323. [DOI] [PubMed] [Google Scholar]
- Mascarello F., Carpenè E., Veggetti A., Rowlerson A., Jenny E. The tensor tympani muscle of cat and dog contains IIM and slow-tonic fibres: an unusual combination of fibre types. J Muscle Res Cell Motil. 1982 Sep;3(3):363–374. doi: 10.1007/BF00713043. [DOI] [PubMed] [Google Scholar]
- Mascarello F., Veggetti A., Cerpenè E., Rowlerson A. An immunohistochemical study of the middle ear muscles of some carnivores and primates, with special reference to the IIM and slow-tonic fibre types. J Anat. 1983 Aug;137(Pt 1):95–108. [PMC free article] [PubMed] [Google Scholar]
- Rowlerson A., Mascarello F., Veggetti A., Carpene E. The fibre-type composition of the first branchial arch muscles in Carnivora and Primates. J Muscle Res Cell Motil. 1983 Aug;4(4):443–472. doi: 10.1007/BF00711949. [DOI] [PubMed] [Google Scholar]
- Sartore S., Mascarello F., Rowlerson A., Gorza L., Ausoni S., Vianello M., Schiaffino S. Fibre types in extraocular muscles: a new myosin isoform in the fast fibres. J Muscle Res Cell Motil. 1987 Apr;8(2):161–172. doi: 10.1007/BF01753992. [DOI] [PubMed] [Google Scholar]
- Snow D. H., Billeter R., Mascarello F., Carpenè E., Rowlerson A., Jenny E. No classical type IIB fibres in dog skeletal muscle. Histochemistry. 1982;75(1):53–65. doi: 10.1007/BF00492533. [DOI] [PubMed] [Google Scholar]
- Soussi-Yanicostas N., Barbet J. P., Laurent-Winter C., Barton P., Butler-Browne G. S. Transition of myosin isozymes during development of human masseter muscle. Persistence of developmental isoforms during postnatal stage. Development. 1990 Feb;108(2):239–249. doi: 10.1242/dev.108.2.239. [DOI] [PubMed] [Google Scholar]
- Veggetti A., Mascarello F., Carpenè E. A comparative histochemical study of fibre types in middle ear muscles. J Anat. 1982 Sep;135(Pt 2):333–352. [PMC free article] [PubMed] [Google Scholar]
- Whalen R. G., Sell S. M., Butler-Browne G. S., Schwartz K., Bouveret P., Pinset-Härstöm I. Three myosin heavy-chain isozymes appear sequentially in rat muscle development. Nature. 1981 Aug 27;292(5826):805–809. doi: 10.1038/292805a0. [DOI] [PubMed] [Google Scholar]
- van den Berge H., Wirtz P. Detailed morphology of the tensor tympani muscle of the rat. An integrated light microscopical, morphometrical, histochemical, immunohistochemical and electron microscopical study in relation to function. J Anat. 1989 Jun;164:215–228. [PMC free article] [PubMed] [Google Scholar]