Abstract
Linder's argyrophil method, recently developed to stain nervous structures, is useful in the histochemical study of amine- and/or peptide-producing neuroendocrine (APUD) cells. On sections from various organs of four animal species Linder's method worked well and rapidly stained the neuroendocrine cells yellow, red or black; it stained black nervous structures against a pale yellow background. Double staining of single sections from Bouin-fixed gastric mucosa of rabbits demonstrated the correspondence of both Linder- and Grimelius-positive cells. Rapidity of application, intensity of impregnation and reproducibility in results are the best features of Linder's method when applied to the study of the neuroendocrine system.
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