Abstract
Linder's argyrophil method, recently developed to stain nervous structures, is useful in the histochemical study of amine- and/or peptide-producing neuroendocrine (APUD) cells. On sections from various organs of four animal species Linder's method worked well and rapidly stained the neuroendocrine cells yellow, red or black; it stained black nervous structures against a pale yellow background. Double staining of single sections from Bouin-fixed gastric mucosa of rabbits demonstrated the correspondence of both Linder- and Grimelius-positive cells. Rapidity of application, intensity of impregnation and reproducibility in results are the best features of Linder's method when applied to the study of the neuroendocrine system.
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- Linder J. E. A simple and reliable method for the silver impregnation of nerves in paraffin sections of soft and mineralized tissues. J Anat. 1978 Dec;127(Pt 3):543–551. [PMC free article] [PubMed] [Google Scholar]
- MAYOR H. D., HAMPTON J. C., ROSARIO B. A simple method for removing the resin from epoxy-embedded tissue. J Biophys Biochem Cytol. 1961 Apr;9:909–910. doi: 10.1083/jcb.9.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rindi G., Buffa R., Sessa F., Tortora O., Solcia E. Chromogranin A, B and C immunoreactivities of mammalian endocrine cells. Distribution, distinction from costored hormones/prohormones and relationship with the argyrophil component of secretory granules. Histochemistry. 1986;85(1):19–28. doi: 10.1007/BF00508649. [DOI] [PubMed] [Google Scholar]
- SINGH I. A MODIFICATION OF THE MASSON-HAMPERL METHOD FOR STAINING OF ARGENTAFFIN CELLS. Anat Anz. 1964 Jul 31;115:81–82. [PubMed] [Google Scholar]