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. 1992 Dec;63(6):1493–1499. doi: 10.1016/S0006-3495(92)81734-4

Dielectric spectroscopy of plant protoplasts

Koji Asami 1, Tohru Yamaguchi 1
PMCID: PMC1262264  PMID: 19431864

Abstract

The relative permittivity and conductivity of the mesophyll protoplasts isolated from Brassica campestris leaves and Tulipa gesneriana petals were measured over a frequency range from 1kHz to 500 MHz.These protoplasts showed a broad dielectric dispersion, which was composed of three subdispersions, termed β1-, β2-, and β3-dispersion in increasing order of frequency.The three subdispersions were assigned to the Maxwell-Wagner dispersion caused by charging processes at the interfaces of the surface and internal membranes; the plasma membrane, the tonoplast, and the membranes of cytoplasmic organelles (e.g., chloroplasts, granules, etc) primarily contribute to the β1-, β2-, and β3-dispersion, respectively. The whole dielectric dispersion curve was satisfactorily interpreted in terms of a spherical cell model taking a large vacuole and cytoplasmic organelles into account. Using this model the capacitances of the plasma membranes and the tonoplasts were estimated to be 0.6-0.7 μF/cm2 and 0.9-1.0 μF/cm2, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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