Abstract
A protected catheter brush introduced by fiberoptic bronchoscopy was used to sample the tracheai and bronchial mucosa in 28 horses with small airway disease. Tracheal and bronchial brushings were examined for the presence of fungi, aerobic and anaerobic bacteria, and a cytoiogical evaluation was also done on fluid collected by the bronchoalveolar lavage (BAL) technique. Microorganisms (bacteria and fungi) were isolated more often in tracheal brushings (53.6%) than in bronchial brushings (10.7%). Anaerobic bacteria were not isolated. Results of this study indicate that fiberoptic bronchoscopy using a protected catheter brush is an easy and practical technique to obtain minimally contaminated samples for isolation of microorganisms from the lower respiratory tract of horses. However, no association was observed between isolation of high numbers of microorganisms from the bronchi and severity of small airway disease.
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Selected References
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- Bartlett J. G., Alexander J., Mayhew J., Sullivan-Sigler N., Gorbach S. L. Should fiberoptic bronchoscopy aspirates be cultured? Am Rev Respir Dis. 1976 Jul;114(1):73–78. doi: 10.1164/arrd.1976.114.1.73. [DOI] [PubMed] [Google Scholar]
- Bartlett J. G., Gorbach S. L., Finegold S. M. The bacteriology of aspiration pneumonia. Am J Med. 1974 Feb;56(2):202–207. doi: 10.1016/0002-9343(74)90598-1. [DOI] [PubMed] [Google Scholar]
- Bass J. B., Jr, Hawkins E. L., Bonner J. R., Pollock H. M. Use of a bronchoscopic protected catheter technique in the clinical evaluation of a new antibiotic. Diagn Microbiol Infect Dis. 1983 Jun;1(2):95–106. doi: 10.1016/0732-8893(83)90038-x. [DOI] [PubMed] [Google Scholar]
- Beech J. Technique of tracheobronchial aspiration in the horse. Equine Vet J. 1981 Apr;13(2):136–137. doi: 10.1111/j.2042-3306.1981.tb04144.x. [DOI] [PubMed] [Google Scholar]
- CARPENTER C. P., SHAFFER C. B. A study of the polyethylene glycols as vehicles for intramuscular and subcutaneous Injection. J Am Pharm Assoc Am Pharm Assoc. 1952 Jan;41(1):27–29. doi: 10.1002/jps.3030410112. [DOI] [PubMed] [Google Scholar]
- Chastre J., Viau F., Brun P., Pierre J., Dauge M. C., Bouchama A., Akesbi A., Gibert C. Prospective evaluation of the protected specimen brush for the diagnosis of pulmonary infections in ventilated patients. Am Rev Respir Dis. 1984 Nov;130(5):924–929. doi: 10.1164/arrd.1984.130.5.924. [DOI] [PubMed] [Google Scholar]
- Cook W. R. Chronic bronchitis and alveolar emphysema in the horse. Vet Rec. 1976 Dec 4;99(23):448–451. doi: 10.1136/vr.99.23.448. [DOI] [PubMed] [Google Scholar]
- Derksen F. J., Brown C. M., Sonea I., Darien B. J., Robinson N. E. Comparison of transtracheal aspirate and bronchoalveolar lavage cytology in 50 horses with chronic lung disease. Equine Vet J. 1989 Jan;21(1):23–26. doi: 10.1111/j.2042-3306.1989.tb02084.x. [DOI] [PubMed] [Google Scholar]
- Gerger H. Chronic pulmonary disease in the horse. Equine Vet J. 1973 Jan;5(1):26–33. [PubMed] [Google Scholar]
- Hayes D. A., McCarthy L. C., Friedman M. Evaluation of two bronchofiberscopic methods of culturing the lower respiratory tract. Am Rev Respir Dis. 1980 Aug;122(2):319–323. doi: 10.1164/arrd.1980.122.2.319. [DOI] [PubMed] [Google Scholar]
- Kahn F. W., Jones J. M. Analysis of bronchoalveolar lavage specimens from immunocompromised patients with a protocol applicable in the microbiology laboratory. J Clin Microbiol. 1988 Jun;26(6):1150–1155. doi: 10.1128/jcm.26.6.1150-1155.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAURENZI G. A., POTTER R. T., KASS E. H. Bacteriologic flora of the lower respiratory tract. N Engl J Med. 1961 Dec 28;265:1273–1278. doi: 10.1056/NEJM196112282652601. [DOI] [PubMed] [Google Scholar]
- Mair T. S. Value of tracheal aspirates in the diagnosis of chronic pulmonary diseases in the horse. Equine Vet J. 1987 Sep;19(5):463–465. doi: 10.1111/j.2042-3306.1987.tb02645.x. [DOI] [PubMed] [Google Scholar]
- Mansmann R. A., Knight H. D. Transtracheal aspiration in the horse. J Am Vet Med Assoc. 1972 Jun 1;160(11):1527–1529. [PubMed] [Google Scholar]
- Mansmann R. A., Strouss A. A. Evaluation of transtracheal aspiration in the horse. J Am Vet Med Assoc. 1976 Sep 15;169(6):631–633. [PubMed] [Google Scholar]
- Nuytten J., Muylle E., Oyaert W., van den Hende C., Vlaminck K., de Keersmaecker F. Cytology, bacteriology and phagocytic capacity of tracheo-bronchial aspirates in healthy horses and horses with chronic obstructive pulmonary disease (COPD). Zentralbl Veterinarmed A. 1983 Feb;30(2):114–120. doi: 10.1111/j.1439-0442.1983.tb00683.x. [DOI] [PubMed] [Google Scholar]
- PECORA D. V. A COMPARISON OF TRANSTRACHEAL ASPIRATION WITH OTHER METHODS OF DETERMINING THE BACTERIAL FLORA OF THE LOWER RESPIRATORY TRACT. N Engl J Med. 1963 Sep 26;269:664–666. doi: 10.1056/NEJM196309262691304. [DOI] [PubMed] [Google Scholar]
- PECORA D. V., YEGIAN D. Bacteriology of the lower respiratory tract in health and chronic diseases. N Engl J Med. 1958 Jan 9;258(2):71–74. doi: 10.1056/NEJM195801092580204. [DOI] [PubMed] [Google Scholar]
- Reynolds H. Y. Bronchoalveolar lavage. Am Rev Respir Dis. 1987 Jan;135(1):250–263. doi: 10.1164/arrd.1987.135.1.250. [DOI] [PubMed] [Google Scholar]
- Reynolds H. Y., Newball H. H. Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage. J Lab Clin Med. 1974 Oct;84(4):559–573. [PubMed] [Google Scholar]
- Sweeney C. R., Beech J., Roby K. A. Bacterial isolates from tracheobronchial aspirates of healthy horses. Am J Vet Res. 1985 Dec;46(12):2562–2565. [PubMed] [Google Scholar]
- Sweeney C. R., Sweeney R. W., 3rd, Benson C. E. Comparison of bacteria isolated from specimens obtained by use of endoscopic guarded tracheal swabbing and percutaneous tracheal aspiration in horses. J Am Vet Med Assoc. 1989 Nov 1;195(9):1225–1229. [PubMed] [Google Scholar]
- Whitwell K. E., Greet T. R. Collection and evaluation of tracheobronchial washes in the horse. Equine Vet J. 1984 Nov;16(6):499–508. doi: 10.1111/j.2042-3306.1984.tb02000.x. [DOI] [PubMed] [Google Scholar]
- Wimberley N. W., Bass J. B., Jr, Boyd B. W., Kirkpatrick M. B., Serio R. A., Pollock H. M. Use of a bronchoscopic protected catheter brush for the diagnosis of pulmonary infections. Chest. 1982 May;81(5):556–562. doi: 10.1378/chest.81.5.556. [DOI] [PubMed] [Google Scholar]
- Wimberley N., Faling L. J., Bartlett J. G. A fiberoptic bronchoscopy technique to obtain uncontaminated lower airway secretions for bacterial culture. Am Rev Respir Dis. 1979 Mar;119(3):337–343. doi: 10.1164/arrd.1979.119.3.337. [DOI] [PubMed] [Google Scholar]
