Skip to main content
NCBI home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1991 Apr;55(2):143–149.

Antiviral activity against transmissible gastroenteritis virus, and cytotoxicity, of natural porcine interferons alpha and beta.

H M Weingartl 1, J B Derbyshire 1
PMCID: PMC1263434  PMID: 1653103

Abstract

Porcine interferon (POIFN)-alpha prepared in primed peripheral blood leukocyte cultures induced with Newcastle disease virus and POIFN-beta from PK-15 cell cultures induced with polyinosinic:polycytidylic acid were partially purified by precipitation with potassium thiocyanate and anion exchange chromatography. Mean purification factors in terms of units of POIFN per mg of protein, of 37 and 12 were obtained for POIFN-alpha and POIFN-beta respectively. In yield reduction assays in swine testis and pig kidney cell cultures, POIFN-alpha and POIFN-beta had greater antiviral activity against vesicular stomatitis virus than against transmissible gastroenteritis virus (TGEV). The antiviral effects were greater at higher concentrations of interferon (IFN), and when the IFN treatments were continued postinfection. Porcine interferon-beta showed greater antiviral activity against TGEV than POIFN-alpha, but this may have been partly due to cytotoxicity. There were no major differences in the antiviral activities of crude and partially purified IFN preparations. Both types of IFN showed antiviral activity against TGEV in yield reduction assays in porcine intestinal explant and intestinal epithelial cell cultures. Crude POIFN-beta was found to be rapidly cytotoxic, especially in porcine cells, and some fractions of partially purified POIFN-beta were also cytotoxic. The cytotoxicity of POIFN-beta was partially neutralized by antibodies against human IFN-beta, but human IFN-beta was not cytotoxic for porcine or bovine cells.

Full text

PDF
143

Images in this article

144Fig. 1.
on p.144
147Fig. 2.
on p.147

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albright D. J., Whalen R. A., Blacklow N. R. Sensitivity of human foetal intestine to interferon. Nature. 1974 Jan 25;247(5438):218–220. doi: 10.1038/247218a0. [DOI] [PubMed] [Google Scholar]
  2. Billiau A., Joniau M., De Somer P. Mass production of human interferon in diploid cells stimulated by poly-I:C. J Gen Virol. 1973 Apr;19(1):1–8. doi: 10.1099/0022-1317-19-1-1. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Cantell K., Hirvonen S. Large-scale production of human leukocyte interferon containing 10(8) units per ml. J Gen Virol. 1978 Jun;39(3):541–543. doi: 10.1099/0022-1317-39-3-541. [DOI] [PubMed] [Google Scholar]
  5. Carter W. A., Davis L. R., Jr, Chadha K. C., Johnson F. H., Jr Porcine leukocyte interferon and antiviral activity in human cells. Mol Pharmacol. 1979 May;15(3):685–690. [PubMed] [Google Scholar]
  6. Charley B., Laude H. Induction of alpha interferon by transmissible gastroenteritis coronavirus: role of transmembrane glycoprotein E1. J Virol. 1988 Jan;62(1):8–11. doi: 10.1128/jvi.62.1.8-11.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Derbyshire J. B. The interferon sensitivity of selected porcine viruses. Can J Vet Res. 1989 Jan;53(1):52–55. [PMC free article] [PubMed] [Google Scholar]
  8. Familletti P. C., Rubinstein S., Pestka S. A convenient and rapid cytopathic effect inhibition assay for interferon. Methods Enzymol. 1981;78(Pt A):387–394. doi: 10.1016/0076-6879(81)78146-1. [DOI] [PubMed] [Google Scholar]
  9. La Bonnardiere C., Laude H. High interferon titer in newborn pig intestine during experimentally induced viral enteritis. Infect Immun. 1981 Apr;32(1):28–31. doi: 10.1128/iai.32.1.28-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laude H., La Bonnardiere C. Cytocidal effect of interferons on porcine renal cells. J Interferon Res. 1984;4(1):101–110. doi: 10.1089/jir.1984.4.101. [DOI] [PubMed] [Google Scholar]
  11. Lesnick C. E., Derbyshire J. B. Activation of natural killer cells in newborn piglets by interferon induction. Vet Immunol Immunopathol. 1988 Mar;18(2):109–117. doi: 10.1016/0165-2427(88)90053-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loewen K. G., Derbyshire J. B. Interferon induction with polyinosinic:polycytidylic acid in the newborn piglet. Can J Vet Res. 1986 Apr;50(2):232–237. [PMC free article] [PubMed] [Google Scholar]
  13. Loewen K. G., Derbyshire J. B. The effect of interferon induction in parturient sows and newborn piglets on resistance to transmissible gastroenteritis. Can J Vet Res. 1988 Jan;52(1):149–153. [PMC free article] [PubMed] [Google Scholar]
  14. MacLachlan N. J., Anderson K. P. Effect of recombinant DNA-derived bovine alpha-1 interferon on transmissible gastroenteritis virus infection in swine. Am J Vet Res. 1986 May;47(5):1149–1152. [PubMed] [Google Scholar]
  15. Parish C. R., Müllbacher A. Automated colorimetric assay for T cell cytotoxicity. J Immunol Methods. 1983 Mar 11;58(1-2):225–237. doi: 10.1016/0022-1759(83)90277-6. [DOI] [PubMed] [Google Scholar]
  16. Pensaert M., Haelterman E. O., Burnstein T. Transmissible gastroenteritis of swine: virus-intestinal cell interactions. I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection. Arch Gesamte Virusforsch. 1970;31(3):321–334. doi: 10.1007/BF01253767. [DOI] [PubMed] [Google Scholar]
  17. Piasecki E. Properties of natural porcine interferons. J Interferon Res. 1988 Feb;8(1):61–73. doi: 10.1089/jir.1988.8.61. [DOI] [PubMed] [Google Scholar]
  18. Quaroni A., May R. J. Establishment and characterizaton of intestinal epithelial cell cultures. Methods Cell Biol. 1980;21B:403–427. [PubMed] [Google Scholar]
  19. Rubenstein D., Tyrrell D. A., Derbyshire J. B., Collins A. P. Growth of porcine transmissible gastroenteritis (TGE) virus in organ cultures of pig tissue. Nature. 1970 Sep 26;227(5265):1348–1349. doi: 10.1038/2271348a0. [DOI] [PubMed] [Google Scholar]
  20. Stone-Wolff D. S., Yip Y. K., Kelker H. C., Le J., Henriksen-Destefano D., Rubin B. Y., Rinderknecht E., Aggarwal B. B., Vilcek J. Interrelationships of human interferon-gamma with lymphotoxin and monocyte cytotoxin. J Exp Med. 1984 Mar 1;159(3):828–843. doi: 10.1084/jem.159.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weingartl H. M., Derbyshire J. B. The induction and characterization of natural porcine interferons alpha and beta. Can J Vet Res. 1990 Jun;54(3):349–354. [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES