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. 1991 Apr;55(2):168–173.

Endometrial biopsy in Holstein-Friesian dairy cows. III. Bacteriological analysis and correlations with histological findings.

B N Bonnett 1, S W Martin 1, V P Gannon 1, R B Miller 1, W G Etherington 1
PMCID: PMC1263438  PMID: 1884297

Abstract

This study examines the results of bacterial culture from 159 endometrial biopsy samples from 97 commercial dairy cows and correlations between bacteriological and histological findings. Bacteria were isolated from approximately 80% of biopsies taken at day 26 and day 40 postpartum. Eleven percent of biopsies were positive for both aerobic and anaerobic culture. Streptococci, Escherichia coli and Actinomyces pyogenes were the most common isolates. Isolation of A. pyogenes from a biopsy at day 26 was positively correlated with isolation of anaerobic bacteria and segmented cell inflammation in the same biopsy, and with subsequent isolation of A. pyogenes at day 40. There was a strong association between isolation of A. pyogenes and anaerobes at day 26 with increased uterine lesions at day 40. Isolation of alpha hemolytic streptococci (AHS) was negatively correlated with isolation of A. pyogenes and with inflammation. Actinomyces pyogenes and AHS showed opposite associations with mononuclear cell inflammation and lymphocytic foci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonnett B. N., Etherington W. G., Martin S. W., Johnson W. H. The effect of prostaglandin administration to Holstein-Friesian cows at Day 26 postpartum on clinical findings, and histological and bacteriological results of endometrial biopsies at Day 40. Theriogenology. 1990 Apr;33(4):877–890. doi: 10.1016/0093-691x(90)90823-c. [DOI] [PubMed] [Google Scholar]
  2. Bonnett B. N., Miller R. B., Etherington W. G., Martin S. W., Johnson W. H. Endometrial biopsy in Holstein-Friesian dairy cows. I. Technique, histological criteria and results. Can J Vet Res. 1991 Apr;55(2):155–161. [PMC free article] [PubMed] [Google Scholar]
  3. Bonnett B. N., Miller R. B., Martin S. W., Etherington W. G., Buckrell B. C. Endometrial biopsy in Holstein-Friesian dairy cows. II. Correlations between histological criteria. Can J Vet Res. 1991 Apr;55(2):162–167. [PMC free article] [PubMed] [Google Scholar]
  4. Elliott L., McMahon K. J., Gier H. T., Marion G. B. Uterus of the cow after parturition: bacterial content. Am J Vet Res. 1968 Jan;29(1):77–81. [PubMed] [Google Scholar]
  5. Etherington W. G., Martin S. W., Bonnett B., Johnson W. H., Miller R. B., Savage N. C., Walton J. S., Montgomery M. E. Reproductive performance of dairy cows following treatment with cloprostenol 26 and/or 40 days postpartum: a field trial. Theriogenology. 1988;29(3):565–575. doi: 10.1016/s0093-691x(88)80005-0. [DOI] [PubMed] [Google Scholar]
  6. Gibbs R. S., O'DELL T. N., MacGregor R. R., Schwarz R. H., Morton H. Puerperal endometritis: a prospective microbiologic study. Am J Obstet Gynecol. 1975 Apr 1;121(7):919–925. doi: 10.1016/0002-9378(75)90909-6. [DOI] [PubMed] [Google Scholar]
  7. Griffin J. F., Hartigan P. J., Nunn W. R. Non-specific uterine infection and bovine fertility. I. Infection patterns and endometritis during the first seven weeks post-partum. Theriogenology. 1974 Mar;1(3):91–106. doi: 10.1016/0093-691x(74)90052-1. [DOI] [PubMed] [Google Scholar]
  8. Hartigan P. J., Griffin J. F., Nunn W. R. Some observations on Corynebacterium pyogenes infection of the bovine uterus. Theriogenology. 1974 May;1(5):153–167. doi: 10.1016/0093-691x(74)90048-x. [DOI] [PubMed] [Google Scholar]
  9. McGregor J. A., Lawellin D., Franco-Buff A., Todd J. K., Makowski E. L. Protease production by microorganisms associated with reproductive tract infection. Am J Obstet Gynecol. 1986 Jan;154(1):109–114. doi: 10.1016/0002-9378(86)90404-7. [DOI] [PubMed] [Google Scholar]
  10. Messier S., Higgins R., Couture Y., Morin M. Comparison of swabbing and biopsy for studying the flora of the bovine uterus. Can Vet J. 1984 Jul;25(7):283–288. [PMC free article] [PubMed] [Google Scholar]
  11. Olson J. D., Ball L., Mortimer R. G., Farin P. W., Adney W. S., Huffman E. M. Aspects of bacteriology and endocrinology of cows with pyometra and retained fetal membranes. Am J Vet Res. 1984 Nov;45(11):2251–2255. [PubMed] [Google Scholar]
  12. Paisley L. G., Mickelsen W. D., Anderson P. B. Mechanisms and therapy for retained fetal membranes and uterine infections of cows: A review. Theriogenology. 1986 Mar;25(3):353–381. doi: 10.1016/0093-691x(86)90045-2. [DOI] [PubMed] [Google Scholar]
  13. Roberts D. S. The pathogenic synergy of Fusiformis necrophorus and Corynebacterium pyogenes. I. Influence of the leucocidal exotoxin of F. necrophorus. Br J Exp Pathol. 1967 Dec;48(6):665–673. [PMC free article] [PubMed] [Google Scholar]
  14. Roberts D. S. The pathogenic synergy of Fusiformis necrophorus and Corynebacterium pyogenes. II. The response of F. necrophorus to a filterable product of C. pyogenes. Br J Exp Pathol. 1967 Dec;48(6):674–679. [PMC free article] [PubMed] [Google Scholar]
  15. Sagartz J. W., Hardenbrook H. J. A clinical, bacteriologic, and histologic survey of infertile cows. J Am Vet Med Assoc. 1971 Mar 1;158(5):619–622. [PubMed] [Google Scholar]
  16. Studer E., Morrow D. A. Postpartum evaluation of bovine reproductive potential: comparison of findings from genital tract examination per rectum, uterine culture, and endometrial biopsy. J Am Vet Med Assoc. 1978 Feb 15;172(4):489–494. [PubMed] [Google Scholar]

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