Skip to main content
PMC home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1991 Oct;55(4):362–366.

A field trial to evaluate a whole cell vaccine for the prevention of caseous lymphadenitis in sheep and goat flocks.

P I Menzies 1, C A Muckle 1, K A Brogden 1, L Robinson 1
PMCID: PMC1263484  PMID: 1790493

Abstract

A field trial to evaluate a whole cell vaccine for the prevention of caseous lymphadenitis (CLA) in sheep and goats was performed in one goat herd and one sheep flock over a period of three years. In goats, there was a nonstatistically significant trend for fewer cases of CLA in the vaccinated animals compared to the controls. In sheep, from six months to 36 months postinitial vaccination, the proportion of vaccinated sheep that developed CLA was significantly less (p less than 0.05) than in the control sheep. The antibody titers to Corynebacterium pseudotuberculosis as detected by microagglutination assay were significantly different (p less than 0.0001) at all times except at the initial vaccination. Swellings occurred at the vaccination site at an incidence level of 29.6% in goats and 34.1% in sheep. The vaccine appeared to be efficacious in reducing the proportion of sheep that developed CLA when challenged naturally in a field situation.

Full text

PDF
362

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Augustine J. L., Renshaw H. W. Survival of Corynebacterium pseudotuberculosis in axenic purulent exudate on common barnyard fomites. Am J Vet Res. 1986 Apr;47(4):713–715. [PubMed] [Google Scholar]
  2. Batey R. G. Frequency and consequence of caseous lymphadenitis in sheep and lambs slaughtered at a Western Australian abattoir. Am J Vet Res. 1986 Feb;47(2):482–485. [PubMed] [Google Scholar]
  3. Brogden K. A., Chedid L., Cutlip R. C., Lehmkuhl H. D., Sacks J. Effect of muramyl dipeptide on immunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. Am J Vet Res. 1990 Feb;51(2):200–202. [PubMed] [Google Scholar]
  4. Brogden K. A., Cutlip R. C., Lehmkuhl H. D. Comparison of protection induced in lambs by Corynebacterium pseudotuberculosis whole cell and cell wall vaccines. Am J Vet Res. 1984 Nov;45(11):2393–2395. [PubMed] [Google Scholar]
  5. Brogden K. A., Cutlip R. C., Lehmkuhl H. D. Immunogenicity of Corynebacterium pseudotuberculosis and the effect of adjuvants in mice. J Comp Pathol. 1985 Apr;95(2):167–173. doi: 10.1016/0021-9975(85)90003-9. [DOI] [PubMed] [Google Scholar]
  6. Brown C. C., Olander H. J., Biberstein E. L., Morse S. M. Use of a toxoid vaccine to protect goats against intradermal challenge exposure to Corynebacterium pseudotuberculosis. Am J Vet Res. 1986 May;47(5):1116–1119. [PubMed] [Google Scholar]
  7. Cameron C. M., Engelbrecht M. M. Mechanism of immunity to Corynebacterium pseudotuberculosis (Buchanan, 1911) in mice using inactivated vaccine. Onderstepoort J Vet Res. 1971 Jun;38(2):73–81. [PubMed] [Google Scholar]
  8. Cameron C. M., Fuls W. J. Studies on the enhancement of immunity to Corynebacterium pseudotuberculosis. Onderstepoort J Vet Res. 1973 Sep;40(3):105–113. [PubMed] [Google Scholar]
  9. Cameron C. M., Minnaar J. L., Engelbrecht M. M., Purdom M. R. Immune response of merino sheep to inactivated Corynebacterium pseudotuberculosis vaccine. Onderstepoort J Vet Res. 1972 Mar;39(1):11–24. [PubMed] [Google Scholar]
  10. Cameron C. M., Minnaar J. L., Purdom M. R. Immunizing properties of Corynebacterium pseudotuberculosis cell walls. Onderstepoort J Vet Res. 1969 Dec;36(2):211–216. [PubMed] [Google Scholar]
  11. Dohoo I. R., Curtis R. A., Finley G. G. A survey of sheep diseases in Canada. Can J Comp Med. 1985 Jul;49(3):239–247. [PMC free article] [PubMed] [Google Scholar]
  12. Ellis T. M., Sutherland S. S., Wilkinson F. C., Mercy A. R., Paton M. W. The role of Corynebacterium pseudotuberculosis lung lesions in the transmission of this bacterium to other sheep. Aust Vet J. 1987 Sep;64(9):261–263. doi: 10.1111/j.1751-0813.1987.tb15952.x. [DOI] [PubMed] [Google Scholar]
  13. Gates N. L., Everson D. O., Hulet C. V. Effects of thin ewe syndrome on reproductive efficiency. J Am Vet Med Assoc. 1977 Dec 15;171(12):1266–1267. [PubMed] [Google Scholar]
  14. Hein W. R., Cargill C. F. An abattoir survey of diseases of feral goats. Aust Vet J. 1981 Nov;57(11):498–503. doi: 10.1111/j.1751-0813.1981.tb05780.x. [DOI] [PubMed] [Google Scholar]
  15. LeaMaster B. R., Shen D. T., Gorham J. R., Leathers C. W., Wells H. D. Efficacy of Corynebacterium pseudotuberculosis bacterin for the immunologic protection of sheep against development of caseous lymphadenitis. Am J Vet Res. 1987 May;48(5):869–872. [PubMed] [Google Scholar]
  16. Lund A., Almlid T., Steine T., Larsen H. J. Colostral transfer in the goat of antibodies against Corynebacterium pseudotuberculosis and the antibody status of kids during the first 10 months of life. Acta Vet Scand. 1982;23(4):483–489. doi: 10.1186/BF03546767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Menzies P. I., Muckle C. A. The use of a microagglutination assay for the detection of antibodies to Corynebacterium pseudotuberculosis in naturally infected sheep and goat flocks. Can J Vet Res. 1989 Jul;53(3):313–318. [PMC free article] [PubMed] [Google Scholar]
  18. Paton M. W., Mercy A. R., Wilkinson F. C., Gardner J. J., Sutherland S. S., Ellis T. M. The effects of caseous lymphadenitis on wool production and bodyweight in young sheep. Aust Vet J. 1988 Apr;65(4):117–119. doi: 10.1111/j.1751-0813.1988.tb14429.x. [DOI] [PubMed] [Google Scholar]
  19. Peto R., Pike M. C., Armitage P., Breslow N. E., Cox D. R., Howard S. V., Mantel N., McPherson K., Peto J., Smith P. G. Design and analysis of randomized clinical trials requiring prolonged observation of each patient. II. analysis and examples. Br J Cancer. 1977 Jan;35(1):1–39. doi: 10.1038/bjc.1977.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Renshaw H. W., Graff V. P., Gates N. L. Visceral caseous lymphadenitis in thin ewe syndrome: isolation of Corynebacterium, Staphylococcus, and Moraxella spp from internal abscesses in emaciated ewes. Am J Vet Res. 1979 Aug;40(8):1110–1114. [PubMed] [Google Scholar]
  21. Sheikh-Omar A. R., Shah M. Caseous lymphadenitis in sheep imported from Australia for slaughter in Malaysia. Aust Vet J. 1984 Dec;61(12):410–410. doi: 10.1111/j.1751-0813.1984.tb07179.x. [DOI] [PubMed] [Google Scholar]
  22. Stoops S. G., Renshaw H. W., Thilsted J. P. Ovine caseous lymphadenitis: disease prevalence, lesion distribution, and thoracic manifestations in a population of mature culled sheep from western United States. Am J Vet Res. 1984 Mar;45(3):557–561. [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES