Abstract
Skeletal muscle, diaphragm, tongue, esophagus and heart of beef carcasses that were condemned for eosinophilic myositis and those that were unaffected were collected at an abattoir in Colorado and studied to determine the involvement of Sarcocystis spp. All affected carcasses contained similar granulomatous lesions with adjacent infiltrations of leukocytes. Intact or fragments of sarcocysts were found within 32 of 363 granulomas, and whole sarcocysts were present in nearby unaffected muscle cells. Light and electron microscopic examinations revealed that sarcocysts, affected or unaffected by cellular response in condemned carcasses, as well as those found in unaffected carcasses, were consistent with those of S. cruzi. Transmission experiments confirmed that S. cruzi were present in all carcasses, and that dogs, but not cats, were the definitive hosts. The results of pepsin-HCl digestion assays showed that unaffected carcasses that were approved for human consumption generally contained more infective parasites than carcasses that were condemned for eosinophilic myositis. This study provides evidence to support the suggestion that dogs, rather than cats, and unaffected rather than eosinophilic myositis-affected carcasses, have greater potential for contributing to the perpetuation of eosinophilic myositis in the cattle industry.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blank H., Davis C., Collins C. Electron microscopy for the diagnosis of cutaneous viral infections. Br J Dermatol. 1970;83(Suppl):69–80. doi: 10.1111/j.1365-2133.1970.tb12866.x. [DOI] [PubMed] [Google Scholar]
- Bundza A., Feltmate T. E. Eosinophilic myositis/lymphadenitis in slaughter cattle. Can Vet J. 1989 Jun;30(6):514–516. [PMC free article] [PubMed] [Google Scholar]
- Collins G. H., Charleston W. A., Wiens B. G. Studies on Sarcocystis species VI: a comparison of three methods for the detection of Sarcocystis species in muscle. N Z Vet J. 1980 Sep;28(9):173–173. doi: 10.1080/00480169.1980.34740. [DOI] [PubMed] [Google Scholar]
- Dubey J. P. Clinical sarcocystosis in calves fed Sarcocystis hirsuta sporocysts from cats. Vet Pathol. 1983 Jan;20(1):90–98. doi: 10.1177/030098588302000110. [DOI] [PubMed] [Google Scholar]
- Dubey J. P., Fayer R., Speer C. A. Experimental Sarcocystis hominis infection in cattle: lesions and ultrastructure of sarcocysts. J Parasitol. 1988 Oct;74(5):875–879. [PubMed] [Google Scholar]
- Dubey J. P., Speer C. A., Epling G. P. Sarcocystosis in newborn calves fed Sarcocystis cruzi sporocysts from coyotes. Am J Vet Res. 1982 Dec;43(12):2147–2164. [PubMed] [Google Scholar]
- Ely R. W., Fox J. C. Elevated IgG antibody to Sarcocystis cruzi associated with eosinophilic myositis in cattle. J Vet Diagn Invest. 1989 Jan;1(1):53–56. doi: 10.1177/104063878900100115. [DOI] [PubMed] [Google Scholar]
- Fayer R., Dubey J. P. Protective immunity against clinical sarcocystosis in cattle. Vet Parasitol. 1984 Sep;15(3-4):187–201. doi: 10.1016/0304-4017(84)90071-2. [DOI] [PubMed] [Google Scholar]
- Gajadhar A. A., Yates W. D., Allen J. R. Association of eosinophilic myositis with an unusual species of Sarcocystis in a beef cow. Can J Vet Res. 1987 Jul;51(3):373–378. [PMC free article] [PubMed] [Google Scholar]
- Granstrom D. E., Ridley R. K., Baoan Y., Gershwin L. J. Immunodominant proteins of Sarcocystis cruzi bradyzoites isolated from cattle affected or nonaffected with eosinophilic myositis. Am J Vet Res. 1990 Jul;51(7):1151–1155. [PubMed] [Google Scholar]
- Granstrom D. E., Ridley R. K., Yao B., Gershwin L. J., Briggs D. J. Immunofluorescent localization of Sarcocystis cruzi antigens, IgG and IgE, in lesions of eosinophilic myositis in cattle. J Vet Diagn Invest. 1990 Apr;2(2):147–149. doi: 10.1177/104063879000200216. [DOI] [PubMed] [Google Scholar]
- Mehlhorn H., Heydorn O., Gestrich R. Licht- und elektronenmikroskopische Untersuchungen an Cysten von Sarcocystis fusiformis in der Muskulatur von Kälbern nach experimenteller Infektion mit Oocysten und Sporocysten von Isopora hominis Railliet et Lucet, 1891. 1. Zur Entstehung der Cyste und der Cystenwand. Zentralbl Bakteriol Orig A. 1975;231(1-3):301–322. [PubMed] [Google Scholar]
- Reiten A. C., Jensen R., Griner L. A. Eosinophilic myositis (sarcosporidiosis; sarco) in beef cattle. Am J Vet Res. 1966 Jul;27(119):903–906. [PubMed] [Google Scholar]
- Rimaila-Pärnänen E., Nikander S. Generalized eosinophilic myositis with sarcosporidiosis in a Finnish cow. Nord Vet Med. 1980 Feb;32(2):96–99. [PubMed] [Google Scholar]