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. 1992 Jul;56(3):265–268.

Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.

K Kenny 1, F D Bastida 1, N L Norcross 1
PMCID: PMC1263550  PMID: 1423065

Abstract

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein hydrolysate and yeast extract in an atmosphere of 7% (v/v) CO2 in air. The assay consisted of a dot immunoblotting technique employing bacterial culture supernatants and a mouse monoclonal antibody specific for alpha-hemolysin. Ninety-four percent (247) of 262 strains were positive for alpha-hemolysin by this method, when cultured in the laboratory. This figure is compared with those obtained in previous studies which typically based their results on the hemolytic patterns of isolates on blood agar plates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adlam C., Ward P. D., McCartney A. C., Arbuthnott J. P., Thorley C. M. Effect immunization with highly purified alpha- and beta-toxins on staphylococcal mastitis in rabbits. Infect Immun. 1977 Aug;17(2):250–256. doi: 10.1128/iai.17.2.250-256.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERNHEIMER A. W., SCHWARTZ L. L. Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol. 1963 Mar;30:455–468. doi: 10.1099/00221287-30-3-455. [DOI] [PubMed] [Google Scholar]
  3. Bernheimer A. W. Staphylococcal alpha toxin. Ann N Y Acad Sci. 1965 Jul 23;128(1):112–123. doi: 10.1111/j.1749-6632.1965.tb11633.x. [DOI] [PubMed] [Google Scholar]
  4. Bramley A. J., Patel A. H., O'Reilly M., Foster R., Foster T. J. Roles of alpha-toxin and beta-toxin in virulence of Staphylococcus aureus for the mouse mammary gland. Infect Immun. 1989 Aug;57(8):2489–2494. doi: 10.1128/iai.57.8.2489-2494.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cassidy P., Harshman S. Purification of staphylococcal alpha-toxin by adsorption chromatography on glass. Infect Immun. 1976 Mar;13(3):982–986. doi: 10.1128/iai.13.3.982-986.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DERBYSHIRE J. B. Studies in immunity to experimental staphylococcal mastitis in the goat and cow. J Comp Pathol. 1960 Apr;70:222–231. doi: 10.1016/s0368-1742(60)80023-9. [DOI] [PubMed] [Google Scholar]
  7. EDWARDS S. J., RIPPON J. E. The characters and distribution of certain staphylo cocci pathogenic for the bovine udder. J Comp Pathol. 1957 Apr;67(2):111–125. doi: 10.1016/s0368-1742(57)80012-5. [DOI] [PubMed] [Google Scholar]
  8. ELEK S. D., LEVY E. The nature of discrepancies between haemolysins in culture filtrates and plate haemolysin patterns of staphylococci. J Pathol Bacteriol. 1954 Jul;68(1):31–40. doi: 10.1002/path.1700680105. [DOI] [PubMed] [Google Scholar]
  9. Herbrink P., Van Bussel F. J., Warnaar S. O. The antigen spot test (AST): a highly sensitive assay for the detection of antibodies. J Immunol Methods. 1982;48(3):293–298. doi: 10.1016/0022-1759(82)90330-1. [DOI] [PubMed] [Google Scholar]
  10. Hájek V., Marsálek E. A study of staphylococci of bovine origin Staphylococcus aureus var. bovis. Zentralbl Bakteriol Orig. 1969 Jan;209(2):154–160. [PubMed] [Google Scholar]
  11. Jasper D. E., Jain N. C. Hemolytic behavior of staphylococci isolated from cows' milk. Can J Comp Med Vet Sci. 1966 Mar;30(3):63–70. [PMC free article] [PubMed] [Google Scholar]
  12. Joshi N., Dale D. G. Some Microbiological Aspects of Staphylococcal Mastitis. Can J Comp Med Vet Sci. 1963 Mar;27(3):61–68. [PMC free article] [PubMed] [Google Scholar]
  13. LOKEN K. I., HOYT H. H. Studies on bovine staphylococcic mastitis. I. Characterization of staphylococci. Am J Vet Res. 1962 May;23:534–540. [PubMed] [Google Scholar]
  14. Loeffler D. A., Norcross N. L. Use of enzyme-linked immunosorbent assay to measure bovine milk and serum antibodies to alpha toxin, beta toxin, and capsular antigens of Staphylococcus aureus. Vet Immunol Immunopathol. 1987 Feb;14(2):145–156. doi: 10.1016/0165-2427(87)90049-3. [DOI] [PubMed] [Google Scholar]
  15. MARKS J., VAUGHAN A. C. T. Staphylococcal delta-haemolysin. J Pathol Bacteriol. 1950 Oct;62(4):597–615. doi: 10.1002/path.1700620411. [DOI] [PubMed] [Google Scholar]
  16. Pankey J. W., Philpot W. N. Hygiene in the prevention of udder infections. I. Comparative efficacy of four teat dips. J Dairy Sci. 1975 Feb;58(2):202–204. doi: 10.3168/jds.S0022-0302(75)84545-0. [DOI] [PubMed] [Google Scholar]
  17. REID W. B., WILSON J. B. A study of the staphylococci associated with the bovine udder. Am J Vet Res. 1959 Sep;20:825–831. [PubMed] [Google Scholar]
  18. SLANETZ L. W., BARTLEY C. H., ALLEN F. E. VACCINATION OF DAIRY CATTLE AGAINST STAPHYLOCOCCIC MASTITIS. Am J Vet Res. 1963 Sep;24:923–932. [PubMed] [Google Scholar]
  19. SLANETZ L. W., BARTLEY C. H. The diagnosis of staphylococcal mastitis, with special reference to the characteristics of mastitis staphylococci. J Infect Dis. 1953 Mar-Apr;92(2):139–151. doi: 10.1093/infdis/92.2.139. [DOI] [PubMed] [Google Scholar]
  20. ST GEORGE C., RUSSELL K. E., WILSON J. B. Characteristics of staphylococci from bovine milk. J Infect Dis. 1962 Jan-Feb;110:75–79. doi: 10.1093/infdis/110.1.75. [DOI] [PubMed] [Google Scholar]
  21. Thelestam M., Blomqvist L. Staphylococcal alpha toxin--recent advances. Toxicon. 1988;26(1):55–65. doi: 10.1016/0041-0101(88)90137-7. [DOI] [PubMed] [Google Scholar]
  22. Ward P. D., Adlam C., McCartney A. C., Arbuthnott J. P., Thorley C. M. A histopathological study of the effects of highly purified staphlococcal alpha and beta toxins on the lactating mammary gland and skin of the rabbit. J Comp Pathol. 1979 Apr;89(2):169–177. doi: 10.1016/0021-9975(79)90056-2. [DOI] [PubMed] [Google Scholar]
  23. Ziv G., Risenberg-Tirer R. Hemolysin and lysozyme production by staphylococci isolated from bovine udders. Ann Rech Vet. 1970;1(2):161–166. [PubMed] [Google Scholar]

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