Abstract
Thirty-eight clinical isolates of Pasteurella multocida, recovered from a continuous flow, farrow-to-finish swine herd, were characterized by capsular serotyping and restriction endonuclease analysis (REA) in order to study the epidemiology of P. multocida pneumonia. Twenty-three of the 38 isolates obtained in the study belonged to serotype A. They displayed three REA patterns after digestion with HpaII, of which one designated A-3 represented 70% of the samples. The remaining 15 isolates were serotype D. Four different REA patterns were observed in the type D isolates. The REA type D-1 was most prevalent and accounted for 47% of the serotype D isolates. All serotype A isolates were nontoxigenic, whereas five (33%) of the serotype D isolates were toxigenic. Vertical transmission of P. multocida could not be demonstrated, and was probably not a major route of infection. The results of this study suggest that strains of P. multocida virulent for pigs exist and cause swine pneumonic pasteurellosis in continuous flow herds by horizontal transmission.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Carter G. R., Rundell S. W. Identification of type A strains of P multocida using staphylococcal hyaluronidase. Vet Rec. 1975 Apr 12;96(15):343–343. doi: 10.1136/vr.96.15.343. [DOI] [PubMed] [Google Scholar]
- Carter G. R., Subronto P. Identification of type D strains of Pasteurella multocida with acriflavine. Am J Vet Res. 1973 Feb;34(2):293–294. [PubMed] [Google Scholar]
- Heddleston K. L., Gallagher J. E., Rebers P. A. Fowl cholera: gel diffusion precipitin test for serotyping Pasteruella multocida from avian species. Avian Dis. 1972 Jul-Sep;16(4):925–936. [PubMed] [Google Scholar]
- Maheswaran S. K., Thies E. S. Influence of encapsulation on phagocytosis of Pasteurella multocida by bovine neutrophils. Infect Immun. 1979 Oct;26(1):76–81. doi: 10.1128/iai.26.1.76-81.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pijoan C., Lastra A., Ramirez C., Leman A. D. Isolation of toxigenic strains of Pasteurella multocida from lungs of pneumonic swine. J Am Vet Med Assoc. 1984 Sep 1;185(5):522–523. [PubMed] [Google Scholar]
- Pijoan C., Morrison R. B., Hilley H. D. Serotyping of Pasteurella multocida isolated from swine lungs collected at slaughter. J Clin Microbiol. 1983 Jun;17(6):1074–1076. doi: 10.1128/jcm.17.6.1074-1076.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pijoan C., Trigo F. Bacterial adhesion to mucosal surfaces with special reference to Pasteurella multocida isolates from atrophic rhinitis. Can J Vet Res. 1990 Apr;54 (Suppl):S16–S21. [PubMed] [Google Scholar]
- Zhao G., Pijoan C., Murtaugh M. P., Molitor T. W. Use of restriction endonuclease analysis and ribotyping to study epidemiology of Pasteurella multocida in closed swine herds. Infect Immun. 1992 Apr;60(4):1401–1405. doi: 10.1128/iai.60.4.1401-1405.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]