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Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1993 Oct;57(4):281–287.

Virulence of Salmonella enteritidis phagetypes 4, 8 and 13 and other Salmonella spp. for day-old chicks, hens and mice.

C Poppe 1, W Demczuk 1, K McFadden 1, R P Johnson 1
PMCID: PMC1263641  PMID: 8269367

Abstract

Virulence of three Canadian poultry strains of Salmonella enteritidis, namely phagetypes (PT) 4, 8 and 13, and one Salmonella heidelberg strain was assessed in orally and intraperitoneally inoculated one-day old chickens and compared to the virulence of a human S. enteritidis PT 4 strain from the United Kingdom (UK). The two PT 4 strains were also compared in orally inoculated adult laying hens. In addition, orally inoculated Balb/c mice were used to evaluate virulence of the above strains and two strains of Salmonella typhimurium containing different plasmids. In orally inoculated one-day old chickens, the UK S. enteritidis PT 4 strain was more virulent than the Canadian PT 4 strain. The UK PT 4 strain was also more virulent and invasive in adult laying hens than the Canadian PT 4 strain. The S. enteritidis PT 8 strain and one S. typhimurium strain isolated from a chicken hatchery were the most virulent for orally inoculated Balb/c mice. This strain of S. typhimurium contained the 60 megadalton plasmid associated with virulence for Balb/c mice which was not present in the S. typhimurium strain isolated from a pig with septicemic disease.

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Selected References

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  1. Barrow P. A., Huggins M. B., Lovell M. A., Simpson J. M. Observations on the pathogenesis of experimental Salmonella typhimurium infection in chickens. Res Vet Sci. 1987 Mar;42(2):194–199. [PubMed] [Google Scholar]
  2. Cowden J. M., Chisholm D., O'Mahony M., Lynch D., Mawer S. L., Spain G. E., Ward L., Rowe B. Two outbreaks of Salmonella enteritidis phage type 4 infection associated with the consumption of fresh shell-egg products. Epidemiol Infect. 1989 Aug;103(1):47–52. doi: 10.1017/s095026880003034x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finlay B. B., Falkow S. Salmonella as an intracellular parasite. Mol Microbiol. 1989 Dec;3(12):1833–1841. doi: 10.1111/j.1365-2958.1989.tb00170.x. [DOI] [PubMed] [Google Scholar]
  4. Gast R. K., Beard C. W. Production of Salmonella enteritidis-contaminated eggs by experimentally infected hens. Avian Dis. 1990 Apr-Jun;34(2):438–446. [PubMed] [Google Scholar]
  5. Gordon R. F., Tucker J. F. The epizootiology of Salmonella menston infection of fowls and the effect of feeding poultry food artificially infected with salmonella. Br Poult Sci. 1965 Jul;6(3):251–264. doi: 10.1080/00071666508415581. [DOI] [PubMed] [Google Scholar]
  6. Gulig P. A., Curtiss R., 3rd Cloning and transposon insertion mutagenesis of virulence genes of the 100-kilobase plasmid of Salmonella typhimurium. Infect Immun. 1988 Dec;56(12):3262–3271. doi: 10.1128/iai.56.12.3262-3271.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gulig P. A., Curtiss R., 3rd Plasmid-associated virulence of Salmonella typhimurium. Infect Immun. 1987 Dec;55(12):2891–2901. doi: 10.1128/iai.55.12.2891-2901.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hinton M., Threlfall E. J., Rowe B. The invasiveness of different strains of Salmonella enteritidis phage type 4 for young chickens. FEMS Microbiol Lett. 1990 Jul;58(2):193–195. doi: 10.1111/j.1574-6968.1990.tb13977.x. [DOI] [PubMed] [Google Scholar]
  9. Hopper S. A., Mawer S. Salmonella enteritidis in a commercial layer flock. Vet Rec. 1988 Sep 24;123(13):351–351. doi: 10.1136/vr.123.13.351. [DOI] [PubMed] [Google Scholar]
  10. Horsfall F. L., Bauer J. H. Individual Isolation of Infected Animals in a Single Room. J Bacteriol. 1940 Oct;40(4):569–580. doi: 10.1128/jb.40.4.569-580.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Humphrey T. J., Baskerville A., Chart H., Rowe B. Infection of egg-laying hens with Salmonella enteritidis PT4 by oral inoculation. Vet Rec. 1989 Nov 18;125(21):531–532. doi: 10.1136/vr.125.21.531. [DOI] [PubMed] [Google Scholar]
  12. Humphrey T. J., Baskerville A., Mawer S., Rowe B., Hopper S. Salmonella enteritidis phage type 4 from the contents of intact eggs: a study involving naturally infected hens. Epidemiol Infect. 1989 Dec;103(3):415–423. doi: 10.1017/s0950268800030818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Humphrey T. J. Growth of salmonellas in intact shell eggs: influence of storage temperature. Vet Rec. 1990 Mar 24;126(12):292–292. [PubMed] [Google Scholar]
  14. Jones G. W., Rabert D. K., Svinarich D. M., Whitfield H. J. Association of adhesive, invasive, and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids. Infect Immun. 1982 Nov;38(2):476–486. doi: 10.1128/iai.38.2.476-486.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Khakhria R., Duck D., Lior H. Distribution of Salmonella enteritidis phage types in Canada. Epidemiol Infect. 1991 Feb;106(1):25–32. doi: 10.1017/s0950268800056417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Levine W. C., Buehler J. W., Bean N. H., Tauxe R. V. Epidemiology of nontyphoidal Salmonella bacteremia during the human immunodeficiency virus epidemic. J Infect Dis. 1991 Jul;164(1):81–87. doi: 10.1093/infdis/164.1.81. [DOI] [PubMed] [Google Scholar]
  17. Lister S. A. Salmonella enteritidis infection in broilers and broiler breeders. Vet Rec. 1988 Sep 24;123(13):350–350. doi: 10.1136/vr.123.13.350. [DOI] [PubMed] [Google Scholar]
  18. László V. G., Csórián E. S., Pászti J. Phage types and epidemiological significance of Salmonella enteritidis strains in Hungary between 1976 and 1983. Acta Microbiol Hung. 1985;32(4):321–340. [PubMed] [Google Scholar]
  19. McIlroy S. G., McCracken R. M., Neill S. D., O'Brien J. J. Control, prevention and eradication of Salmonella enteritidis infection in broiler and broiler breeder flocks. Vet Rec. 1989 Nov 25;125(22):545–548. doi: 10.1136/vr.125.22.545. [DOI] [PubMed] [Google Scholar]
  20. Miller V. L., Beer K. B., Loomis W. P., Olson J. A., Miller S. I. An unusual pagC::TnphoA mutation leads to an invasion- and virulence-defective phenotype in Salmonellae. Infect Immun. 1992 Sep;60(9):3763–3770. doi: 10.1128/iai.60.9.3763-3770.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nakamura M., Sato S., Ohya T., Suzuki S., Ikeda S. Possible relationship of a 36-megadalton Salmonella enteritidis plasmid to virulence in mice. Infect Immun. 1985 Mar;47(3):831–833. doi: 10.1128/iai.47.3.831-833.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. O'Brien J. D. Salmonella enteritidis infection in broiler chickens. Vet Rec. 1988 Feb 27;122(9):214–214. doi: 10.1136/vr.122.9.214-b. [DOI] [PubMed] [Google Scholar]
  23. Olsen J. E., Brown D. J., Baggesen D. L., Bisgaard M. Biochemical and molecular characterization of Salmonella enterica serovar berta, and comparison of methods for typing. Epidemiol Infect. 1992 Apr;108(2):243–260. doi: 10.1017/s0950268800049724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Poppe C., Curtiss R., 3rd, Gulig P. A., Gyles C. L. Hybridization studies with a DNA probe derived from the virulence region of the 60 Mdal plasmid of Salmonella typhimurium. Can J Vet Res. 1989 Oct;53(4):378–384. [PMC free article] [PubMed] [Google Scholar]
  25. Poppe C., Gyles C. L. Relation of plasmids to virulence and other properties of salmonellae from avian sources. Avian Dis. 1987 Oct-Dec;31(4):844–854. [PubMed] [Google Scholar]
  26. Poppe C., Irwin R. J., Forsberg C. M., Clarke R. C., Oggel J. The prevalence of Salmonella enteritidis and other Salmonella spp. among Canadian registered commercial layer flocks. Epidemiol Infect. 1991 Apr;106(2):259–270. doi: 10.1017/s0950268800048408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Poppe C., Johnson R. P., Forsberg C. M., Irwin R. J. Salmonella enteritidis and other Salmonella in laying hens and eggs from flocks with Salmonella in their environment. Can J Vet Res. 1992 Jul;56(3):226–232. [PMC free article] [PubMed] [Google Scholar]
  28. Poppe C., McFadden K. A., Brouwer A. M., Demczuk W. Characterization of Salmonella enteritidis strains. Can J Vet Res. 1993 Jul;57(3):176–184. [PMC free article] [PubMed] [Google Scholar]
  29. Rampling A., Anderson J. R., Upson R., Peters E., Ward L. R., Rowe B. Salmonella enteritidis phage type 4 infection of broiler chickens: a hazard to public health. Lancet. 1989 Aug 19;2(8660):436–438. doi: 10.1016/s0140-6736(89)90604-1. [DOI] [PubMed] [Google Scholar]
  30. Rodrigue D. C., Tauxe R. V., Rowe B. International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect. 1990 Aug;105(1):21–27. doi: 10.1017/s0950268800047609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sadler W. W., Brownell J. R., Fanelli M. J. Influence of age and inoculum level on shed pattern of Salmonella typhimurium in chickens. Avian Dis. 1969 Nov;13(4):793–803. [PubMed] [Google Scholar]
  32. Shipp C. R., Rowe B. A mechanised microtechnique for salmonella serotyping. J Clin Pathol. 1980 Jun;33(6):595–597. doi: 10.1136/jcp.33.6.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Snoeyenbos G. H., Smyser C. F., Van Roekel H. Salmonella infections of the ovary and peritoneum of chickens. Avian Dis. 1969 Aug;13(3):668–670. [PubMed] [Google Scholar]
  34. Suzuki S., Ohmae K., Nakamura M., Sato S., Koeda T., Ohishi K., Muramatsu M. Demonstration of the correlation of a 36-megadalton Salmonella serovar enteritidis plasmid to virulence in mice by reintroduction of the plasmid. Nihon Juigaku Zasshi. 1989 Feb;51(1):203–205. doi: 10.1292/jvms1939.51.203. [DOI] [PubMed] [Google Scholar]
  35. Telzak E. E., Budnick L. D., Greenberg M. S., Blum S., Shayegani M., Benson C. E., Schultz S. A nosocomial outbreak of Salmonella enteritidis infection due to the consumption of raw eggs. N Engl J Med. 1990 Aug 9;323(6):394–397. doi: 10.1056/NEJM199008093230607. [DOI] [PubMed] [Google Scholar]
  36. Timoney J. F., Shivaprasad H. L., Baker R. C., Rowe B. Egg transmission after infection of hens with Salmonella enteritidis phage type 4. Vet Rec. 1989 Dec 9;125(24):600–601. [PubMed] [Google Scholar]
  37. Ward L. R., de Sa J. D., Rowe B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect. 1987 Oct;99(2):291–294. doi: 10.1017/s0950268800067765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Wilcock B. P., Armstrong C. H., Olander H. J. The significance of the serotype in the clinical and pathological features of naturally occurring porcine salmonellosis. Can J Comp Med. 1976 Jan;40(1):80–88. [PMC free article] [PubMed] [Google Scholar]
  39. Williams Smith H., Tucker J. F. The virulence of salmonella strains for chickens: their excretion by infected chickens. J Hyg (Lond) 1980 Jun;84(3):479–488. doi: 10.1017/s0022172400027017. [DOI] [PMC free article] [PubMed] [Google Scholar]

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